Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1405
4
1
gregorian
2026
7
1
25
2
online
1
fulltext
fa
اثر نانوفیتوزوم هسپرتین بر التهاب حاد القاء شده با کاراژینان در موش کوچک آزمایشگاهی: یک مطالعه تجربی
The Effect of Hesperetin Nanophytosome on Carrageenan-Induced Acute Inflammation in Mice: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b> <span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">التهاب، پاسخی بیولوژیکی است که استرس اکسیداتیو در پیشرفت آن نقش دارد. فیتوزومها، نانوحاملهای لیپیدی هستند که میتوانند فعالیت آنتیاکسیدانی پلیفنولها نظیر هسپرتین را ارتقاء دهند. هدف این مطالعه، تعیین اثر ضدالتهابی نانوفیتوزوم هسپرتین در مدل التهاب حاد القاء شده با کاراژینان در موشهای آزمایشگاهی کوچک بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> در این مطالعه تجربی، 35 موش کوچک آزمایشگاهی نر به پنج گروه هفتتایی تقسیم شدند: گروه کنترل هیچ دارویی دریافت نکردند. گروه دوم که برای 14 روز نانوفیتوزوم هسپرتین را با دوز 20 میلیگرم/کیلوگرم به صورت خوراکی و حلال کاراژینان را به صورت تزریق در کف پا دریافت کرد. گروه سوم، کاراژینان </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">1 درصد </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">بهصورت تزریقی در زیر پوست کف پا دریافت کرد. گروه چهارم و پنجم که 14 روز پس از دریافت خوراکی هسپرتین و نانوفیتوزوم آن، کارا</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">ژینان را به صورت تزریق در کف پا دریافت کردند. جهت بررسی وضعیت التهابی از آزمون صفحه داغ استفاده شد. فعالیت آنزیمهای آنتیاکسیدانی و نیز میزان گلوتاتیون و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:120%">MDA</span></span><span style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> اندازهگیری شد. </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">داده</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:120%"></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">ها با استفاده از آنالیز واریانس یکطرفه و آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:120%">Tukey</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شد.</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> نانوفیتوزوم هسپرتین کاهش تأخیر در پاسخ به درد را در مقایسه با کاراژینان افزایش داد. هسپرتین و نانوفیتوزوم آن توانستند موجب کاهش سطح </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:120%">MDA</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> و افزایش سطح گلوتاتیون و افزایش</span></span></span> <span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">فعالیت آنزیمهای کاتالاز، سوپراکسید دیسموتاز و گلوتاتیون پراکسیداز، در مقایسه با گروه دریافت کننده کاراژینان شوند (05/٠</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:120%">P<</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> مطالعه حاضر نشان داد احتمالاً نانوفیتوزوم هسپرتین اثرات ضد التهابی خود را از طریق تعدیل مسیر استرس اکسیداتیو اعمال کرده است.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> التهاب، کاراژینان، هسپرتین، موش کوچک آزمایشگاهی، نانوفیتوزوم، استرس اکسیداتیو</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:140%">Background and Objectives:</span></span></b></a> <span style="font-size:9.0pt"><span style="line-height:140%">Oxidative stress plays a critical role in the progression of inflammatory responses. Phytosomal delivery systems, as lipid-based nanocarriers, offer a potent strategy to augment the antioxidant activity of polyphenolic compounds like hesperetin This study was designed and conducted to evaluate the anti-inflammatory effects of hesperetin nano-phytosome in a carrageenan-induced acute inflammation model in small laboratory mice.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Materials and Methods: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">In this experimental study, 35 male small laboratory mice were randomly divided into five groups (n=7): the control group received no treatment; the second group received hesperetin nano-phytosome orally at a dose of 20 mg/kg for 14 days and carrageenan vehicle via intraplantar injection; the third group received a subplantar injection of 1% carrageenan; and the fourth and fifth groups received oral hesperetin and hesperetin nano-phytosome for 14 days, followed by carrageenan injection. Inflammatory pain was evaluated using the hot plate test. At the end of the experiment, antioxidant enzyme activities, as well as glutathione and malondialdehyde levels, were measured. Data were analyzed using one-way analysis of variance followed by Tukey’s post hoc test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Results: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Hesperetin nano-phytosome significantly increased pain response latency compared with the carrageenan group. Both hesperetin and its nano-phytosome significantly reduced malondialdehyde levels and increased glutathione levels as well as the activities of catalase, superoxide dismutase, and glutathione peroxidase compared with the carrageenan-treated group (p<0.05).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Conclusion: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">The results of the present study indicate that hesperetin nano-phytosome is likely to exert its anti-inflammatory effects through modulation of oxidative stress.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Keywords: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Inflammation, Carrageenan, Hesperetin, Mice, Nanophytosome, Oxidative stress</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span style="font-size:9.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Funding: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">This study did not receive any funds.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Conflict of interest: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Ethical considerations: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">This study was approved by the Ethics Committee of the University of Mazandaran, Babolsar, Iran (IR.UMZ.REC.1401.003).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Authors’ contributions:</span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Conceptualization:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Akbar Hajizadeh Moghaddam</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Methodology:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Vahid Hasantabar</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Data collection:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Simin Sadeghi Mahali </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Formal analysis:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Sedigheh Khanjani Jelodar</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Supervision:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Akbar Hajizadeh Moghaddam</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Project administration:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Sedigheh Khanjani Jelodar</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Writing – original draft:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Simin Sadeghi Mahali </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Writing – review & editing:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Akbar Hajizadeh Moghaddam, Sedigheh Khanjani Jelodar</span></span><span style="font-size:9.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
التهاب, کاراژینان, هسپرتین, موش کوچک آزمایشگاهی, نانوفیتوزوم, استرس اکسیداتیو
Inflammation, Carrageenan, Hesperetin, Mice, Nanophytosome, Oxidative stress
103
116
http://journal.rums.ac.ir/browse.php?a_code=A-10-2093-5&slc_lang=fa&sid=1
Simin
Sadeghi
سیمین
صادقی
sadeghy.simin73@gmail.com
6900319475328460063086
0009-0007-4936-9481
No
Mazandaran University
دانشگاه مازندران
Akbar
Hajizadeh Moghaddam
اکبر
حاجی زاده مقدم
a.hajizadeh@umz.ac.ir
6900319475328460063087
6900319475328460063087
Yes
Mazandaran University
دانشگاه مازندران
Sedigheh
Khanjani Jelodar
صدیقه
خانجانی جلودار
s.khanjani@umz.ac.ir
6900319475328460063088
6900319475328460063088
No
Mazandaran University
دانشگاه مازندران
Vahid
Hasantabar
وحید
حسن تبار
vahidhasantabar@yahoo.com
6900319475328460063089
6900319475328460063089
No
Mazandaran University
دانشگاه مازندران