Volume 11, Number 2 (6-2012)                   JRUMS 2012, 11(2): 93-102 | Back to browse issues page


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Ghalebi S, Hassanshahi G, Tabatabai Naini S, Esmaeili Nadimi A, Vazirinejad R, Rezaeian M, et al . Evaluation of the Changes Pattern in Serum Levels of SDF-1α and IP-10 in Dogs Animal Model of Experimental Myocardial Infarction. JRUMS. 2012; 11 (2) :93-102
URL: http://journal.rums.ac.ir/article-1-1354-en.html

Abstract:   (7345 Views)

  Background and Objectives : Since chemokines expression is one of the most inflammatory responses to injury in the myocardium , this project was aimed to find out the pattern of expression of SDF-1 and IP-10 in dog model of experimental ischemia to extend the results of this model to clinical applications.

  Material and Methods: In this experimental study eighteen male dogs aged (6 - 12 months), were randomized in three groups. 1: infarction , Left Anterior Descending Coronary Artery (LAD) was ligated. 2: sham, received the same surgical procedure, excluding LAD ligation. 3: control, was held intact in the same condition. Blood samples were harvested 24 hours before operation, at the time of ligation, 2, 24 and 72 hours and 7 and 28 days after ligation in all groups and serum level of these chemokines was measured by means of ELISA test.

  Results: In infarction group the serum level of IP-10 was significantly increased (p ≤ 0.05) at the 2, 24 and 72 hours after MI and reached at peak after 2 hours. In this group, serum level of SDF-1 α at 24 and 72 hours and 7 and 28 days after MI was significantly increased (p ≤ 0.05). The peak of increase was seen in the 72 hours after MI.

  Conclusion: U pregulation of angiostatic factors, such as IP-10, in the first few hours following injury may inhibit premature angiogenesis. Suppression of IP-10 synthesis during the healing phase may allow formation of the wound neovessels, whereas high plasma levels of SDF-1 α are clinically associated with start of angiogenesis activities.

  Key word: SDF-1α , IP-10, Experimental MI, Dog, Chemokine

  Funding: This research was funded by both Rafsanjan University of Medical Sciences and Faculty of Veterinary Medicine of Shiraz University.

  Conflict of interest : None declared.

  Ethical approval: The Ethical Committee of Rafsanjan University of Medical Sciences approved the study.

  

  How to cite this article : Ghalebi S.R, Hassanshahi G.H, Tabatabai Naini S.A, Esmaeili Nadimi A, Vazirinejad, Rezaeian M, Shamsizadeh A, Akrami Mohajeri F. Evaluation of the Changes Pattern in Serum Levels of SDF-1α and IP-10 in Dogs Animal Model of Experimental Myocardial Infarction. J Rafsanjan Univ Med scie 2012 11(2): 93-. [Farsi]

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Type of Study: Research | Subject: Microbiology
Received: 2012/06/5

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