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URL: http://journal.rums.ac.ir/article-1-2037-en.html
Effect of Garlic Aqueous Extract on Markers of Oxidative Stress in Diabetic Rats Testes
R. Salimnejad[1], M. Jalali[2], M.R. Nikravesh[3], A.R. Fazel3
Received: 25/01/2014 Sent for Revision: 15/03/2014 Received Revised Manuscript: 31/05/2014 Accepted: 24/06/2014
Background and Objective: Diabetes mellitus is one of the most prevalent metabolic diseases and causes testicular damage by rising oxidative stress. Using antioxidants reduces oxidative stress and the amount of testicular damage. In this study, the effect of garlic extract on markers of oxidative stress in diabetic rats testes has been studied.
Materials and Methods: In this experimental study, 24 male Wistar rats were randomly divided into three groups: Control, Diabetic and Diabetic with garlic extract. Control and diabetic groups received distilled water (1ml/100g BW) and diabetic with garlic extract received garlic extract (1ml/100g BW,equivalent to 400 mg/100 g BW) for 6 weeks. Diabete was induced by a single intraperitoneal injection of streptozotocin at a dosage of 60 mg/kg BW. After 6 weeks, the right testis removed and activity of oxidative stress enzymes including malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were studied.
Results: Treatment of diabetic rats with garlic extract significantly reduced blood glucose levels (p<0.001). Also during diabete, the level of MDA enzymes activity was increased and the level of SOD and CAT enzymes activity decreased in the testes. Treatment with the garlic extract significantly improved these parameters (p<0.001).
Conclusion: The results of this study confirm the antioxidant role of garlic extract on improving the testicular damage caused by diabete.
Key words: Oxidative Stress, Diabetes Mellitus, Garlic Extract, Testis
Funding: This research was funded by Mashhad University of Medical sciences.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Mashhad University of Medical Sciences approved the study.
How to cite this article: Salimnejad R, Jalali M, Nikravesh MR, Fazel AR. Effect of Garlic Aqueous Extract on Markers of Oxidative Stress in Diabetic Rats Testes. J RafsanjanUniv Med Sci 2014; 13(4): 371-82. [Farsi]
[1]- MS Student. Anatomy and Cell Biology Department, Medical Faculty, Mashhad University of Medical sciences, Mashhad, Iran
[2]- Prof., Anatomy and Cell Biology Department, Medical Faculty, Mashhad University of Medical sciences, Mashhad, Iran
(Corresponding Author): Tel: (0511) 8002491, Fax: (0511) 8002484, E-mail: JalaliM@mums.ac.ir
[3]- Prof., Anatomy and Cell Biology Department, Medical Faculty, Mashhad University of Medical sciences, Mashhad, Iran
Received: 2014/01/11 | Accepted: 2014/10/28 | Published: 2014/10/28
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