Volume 14, Number 1 (4-2015)                   JRUMS 2015, 14(1): 77-82 | Back to browse issues page


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Goudarzi G, Azadpour M. Prevalence of Clindamycin Inducible Resistance in Staphylococcus Aureus and Coagulase-Negative Staphylococci Using D-Test: A Short Report . JRUMS. 2015; 14 (1) :77-82
URL: http://journal.rums.ac.ir/article-1-2211-en.html

M.Sc Lorestan University of Medical Sciences
Abstract:   (2623 Views)

Background and Objective: The D-test is performed by placing clindamycin and erythromycin impregnated disks at a standard distance on the agar plate then looking for the flattening of inhibition zone around the clindamycin disk. A positive D-test indicates clindamycin inducible resistance and also predicts treatment failure possibility of this antibiotic in clinic. Prevalence of staphylococcal inducible resistance to clindamycin through the D-test was the aim of this study.

Materials and Methods: In this descriptive-cross-sectional study, inducible resistance to clindamycin was examined on the 150 isolated Staphylococcus aureus (n=103) and coagulase-negative staphylococci (n=47) collected from nasal employees and clinical samples by using D-test. The statistical analysis was performed using chi-square test.

Results: Out of 103 isolated S. aureus, 22 (21.4%) were methicillin-resistant (MRSA). Among the tested samples, 3(2%), 1(0.6%) and 2(1.3%) had D, D+ and HD phenotypes, respectively. Fifty-four (36%) of isolations exhibited resistance to both clindamycin and erythromycin. Prevalence of resistance to clindamycin and erythromycin between isolated MRSA and methicillin-susceptible S. aureus (MSSA) was statistically significant (p<0.05).

Conclusion: Phenotypical resistance to erythromycin is not always attributable to clindamycin resistance. Therefore, staphylococcus strains resistant to erythromycin and susceptible to clindamycin should be subjected to D-test assay.

Key words: Inducible resistance, Clindamycin, Staphylococcus aureus, D-test

 

Funding: This research was funded by Lorestan University of Medical Sciences.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Lorestan University of Medical Sciences approved the study.

 

How to cite this article: Goudarzi Gh.R, Azadpour M. Prevalence of Clindamycin Inducible Resistance in Staphylococcus Aureus and Coagulase-Negative Staphylococci Using D-Test: A Short Report. J RafsanjanUniv Med Sci 2015 14(1): 77-82. [Farsi]

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Type of Study: Research | Subject: علوم آزمايشگاهي
Received: 2014/06/27 | Accepted: 2015/04/26 | Published: 2015/04/26

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