Volume 14, Issue 4 (6-2015)                   JRUMS 2015, 14(4): 257-268 | Back to browse issues page


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Moeini F, Mohammadi-Sichoni M, Shahanipoor K. Evaluation of the Antibacterial Effect of Methanol and Aqueous Extracts of Vaccinium Arctostaphylos Fruit against Salmonella spp in vitro . JRUMS. 2015; 14 (4) :257-268
URL: http://journal.rums.ac.ir/article-1-2228-en.html

MSc Islamic Azad University
Abstract:   (2742 Views)

Background and Objective: Salmonella infections, or salmonellosis such as typhoid, bacteremia, enterocolitis are the major health problem worldwide, especially in developing countries including Iran. This study aimed to evaluate the antibacterial effect of methanol and aqueous extracts of Vaccinium arctostaphylos fruit against Salmonella spp.

Materials and Methods: The present study is an experimental one Methanol and aqueous extracts of Vaccinium arctostaphylos fruit were prepared by maceration and Soxhlet method. Antimicrobial effects of the extracts were evaluated by agar diffusion method against Salmonella typhi (PTCC: 1609) and six clinical strains of Salmonella. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by micro-dilution method.

Results: All of the studied strains were sensitive to the aqueous and methanol extracts of Vaccinium arctostaphylos fruit. The mean zones of inhibition were obtained in ranging from 6.6 to 26.6 mm. Statistical analysis showed a significant difference between the mean diameters of inhibition zone and increased extracts concentrations (p<0.001). The MIC values were ranged from 50-200 mg/ml, whereas the MBC values from 100- 400 mg/ml.

Conclusion: Methanol and aqueous extracts of Vaccinium arctostaphylos fruit had inhibitory activity against Salmonella spp.

Key words: Salmonella, Vaccinium arctostaphylos, Methanol extract, Aqueous extract, Inhibitory effect

 

Funding: This research was funded by Falavarjan Branch, Islamic Azad University.

Conflict of interest: None declared.

Ethical approval: This article does not need permission from the Ethics Committee because the information in this article was derived from a non-animal research.

 

How to cite this article: Moeini F, Mahammadi-Sichani M, Shahanipoor K. Evaluation of the Antibacterial Effect of Methanol and Aqueous Extracts of Vaccinium Arctostaphylos Fruit against Salmonella spp in vitro. J RafsanjanUniv Med Sci 2015 14(4): 257-68. [Farsi]

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Type of Study: Research | Subject: Microbiology
Received: 2014/07/9 | Accepted: 2015/05/9 | Published: 2015/06/23

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