Volume 14, Issue 5 (7-2015)                   JRUMS 2015, 14(5): 427-434 | Back to browse issues page


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Alizadeh S, Amini K. Identification of Virulence Gene Panton-Valentine Leukocidin (PVL) and Resistance to Methicillin (mecA) in Staphylococcus Aureus Isolated from Clinical Specimens: A Short Report. JRUMS. 2015; 14 (5) :427-434
URL: http://journal.rums.ac.ir/article-1-2485-en.html

MSc Islamic Azad University, sirjan
Abstract:   (3172 Views)

Background and Objective: Staphylococcus aureus is a major pathogen of human in the world. Methicillin-resistant Staphylococcus aureus is associated with nosocomial infections. Recently, staphylococcal infections have been associated with the community. The purpose of this study was to isolate and identify Staphylococcus aureus virulence Panton-Valentine leukocidin (PVL) and  methicillin resistance genes in clinical samples using PCR techniques.

Materials and Methods:In this experimental study after collecting samples, it was performed biochemical and microbial tests and then antibiotic susceptibility testing by disk diffusion method according to CLSI guidelines with antibiotics of different groups. To identify the virulence and resistance genes multiplex PCR test was used.

Results: The findings showed that the prevalence of methicillin-resistant gene (mecA) in clinical samples are 50% and PVL 2.5%, respectively. Among the antibiotics, resistance to azithromycin, linezolid, ofloxacin and methicillin were 32.5%, 35%, 27.5% and 27.5%, respectively.

Conclusion :There is a serious threat for public health because of the importance of Staphylococcus aureus as a major pathogen in developing countries and due to the increasing use of antibacterial agents. Multiplex PCR is a simple, sensitive, inexpensive, relatively quick, and very unique test to identify multiple of genes, simultaneously.

Keywords: Staphylococcus aureus, mec A, Panton-Valentine Leukocidin, Multiplex PCR 

 

Funding: This research was funded by Sirjan Science and Research Branch, Islamic Azad University of Sirjan.

Conflict of interest: None declared.

Ethical approval: None declared.

 

How to cite this article: Alizadeh S, Amini K. Identification of Virulence Gene Panton-Valentine Leukocidin (PVL) and Resistance to Methicillin (mecA) in Staphylococcus Aureus Isolated from Clinical Specimens: A Short Report. J Rafsanjan Univ Med Sci 2015 14(5): 427-34. [Farsi]

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Type of Study: Research | Subject: Microbiology
Received: 2015/01/18 | Accepted: 2015/05/27 | Published: 2015/07/15

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