Background
and Objective: Staphylococcus aureus is a major pathogen of human in the world.
Methicillin-resistant Staphylococcus aureus is associated with
nosocomial infections. Recently, staphylococcal infections have been associated
with the community. The purpose of this study was to isolate and identify Staphylococcus
aureus virulence Panton-Valentine leukocidin (PVL) and methicillin resistance genes in clinical
samples using PCR techniques.
Materials
and Methods:In this experimental study after
collecting samples, it was performed biochemical and microbial tests and then antibiotic
susceptibility testing by disk diffusion method according to CLSI guidelines
with antibiotics of different groups. To identify the virulence and resistance
genes multiplex PCR test was used.
Results: The findings showed that the prevalence of methicillin-resistant
gene (mecA) in clinical samples are 50% and PVL 2.5%, respectively. Among the
antibiotics, resistance to azithromycin, linezolid,
ofloxacin and methicillin were 32.5%, 35%,
27.5% and 27.5%, respectively.
Conclusion :There is a serious threat for
public health because of the importance of Staphylococcus aureus as a
major pathogen in developing countries and due to the increasing use of
antibacterial agents. Multiplex PCR is a simple, sensitive, inexpensive,
relatively quick, and very unique test to identify multiple of genes,
simultaneously.
Keywords: Staphylococcus aureus, mec A, Panton-Valentine Leukocidin, Multiplex PCR
Funding:
This research
was funded by Sirjan Science and Research Branch, Islamic Azad University of Sirjan.
Conflict of interest: None declared.
Ethical approval: None declared.
How to cite this article: Alizadeh
S, Amini K. Identification of
Virulence Gene Panton-Valentine Leukocidin (PVL) and Resistance to Methicillin (mecA) in Staphylococcus
Aureus Isolated from Clinical Specimens: A Short Report. J Rafsanjan Univ
Med Sci 2015 14(5): 427-34.
[Farsi]
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