Background
and Objective: Various
diseases and injuries can lead to loss cartilage tissue. Cartilage
tissue engineering based on the use of stem cells which has provided a
promising opportunity to repair damaged tissue. Recently, adipose-derived mesenchymal
stem cells (ADSCs) have captured considerable scientific and clinic interest
because of their easy access, rapid expansion in vitro and ability to
differentiate into diverse cell lines. The aim of this study was to introduce
the pellet culture system as an in vitro model for cartilage engineering
approaches.
Materials and Methods:
This experimental study was performed in Stem Cell laboratory, The Academic
Center for Education, Culture and Research, Qom in 2013. In this study, ADSCs
were isolated and expanded from human adipose tissue. The cells were
centrifuged and the chondrogenic differentiation was performed in pellet
culture system. Viability potential and chondrogenic genes expression were
evaluated by MTT assay and Real-time PCR analysis, respectively. In this study,
data statistical
analysis was carried out by independent T-test.
Results: In this study, our
obtained results using MTT assay and Real-time PCR analysis demonstrated cell
survival ability and proliferation and also the expression of
chondrogenic-specific genes at pellet in comparison with control group.
Conclusion: It is suggested
that this method can be used for induction of chondrogenic.
Key words: Adipose-derived mesenchymal
stem cells, Pellet culture system, Differentiate into chondrocyte
Funding: This research was funded by The Academic
Center for Education, Culture and Research, qom-Iran.
Conflict of interest: None
declared.
Ethical
approval: The
Ethics Committee of The Academic Center for Education, Culture and Research,
qom-Iran
approved the study.
How to cite this article: Tabatabaei Qomi R, Sheykhhasan M, Kalhor N, Ghiasi
M.
Introduction of Pellet Culture System of Human Adipose-Derived Mesenchymal Stem
Cells as an in vitro Model for Cartilage Engineering Approaches. J RafsanjanUniv
Med Sci 2015 14(4): 269-82.
[Farsi]
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