Volume 14, Issue 8 (11-2015)                   JRUMS 2015, 14(8): 701-708 | Back to browse issues page


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Kord Z, Amini K, Parviz M. Determining the Frequency of Panton-Valentine Leukocidin (PVL Genes), and Collagen-Binding Protein (cna) in Staphylococcus Aureus Strains Isolated from Clinical Specimens and Antibiotic Resistance: A Short Report. JRUMS. 2015; 14 (8) :701-708
URL: http://journal.rums.ac.ir/article-1-2618-en.html

Assistant Professor Saveh Branch, Islamic Azad University
Abstract:   (1461 Views)

Background and Objective: Panton-Valentine leukocidin (PVL) toxin is a hemolytic exotoxin that increases the permeability of the cell membrane, thus causing lysis of leukocytes and tissue necrosis. Collagen-binding protein (Cna) adhesion Staphylococcus aureus is responsible for binding to collagen and the major virulence factor in arthritis and osteomyelitis. The purpose of this study was to investigate the presence of antibiotic resistance and virulence genes for PVL, Cna in Staphylococcus aureus strains isolated from clinical specimens by Multiplex PCR.

Materials and Methods: In this cross-sectional, descriptive study, 67 samples of Staphylococcus aureus were isolated. Based on CLSI antimicrobial susceptibility guidline, different antibiotics were tested. Multiplex PCR assay was performed.

Results: Finndings showed that the susceptibility to vancomycin and linezolid antibiotics was 97.1% and maximum resistance is to clindamycin was 23.9%. the frequency of Cna gene in clinical samples was 41.7% while PVL genes were not detected in the samples.

Conclusion: Because of the importance of methicillin resistance Staphylococcus aureus as the cause of infection among people, especially resistant hospital infections, due to increasing use of antibiotics, knowledge of the level of resistance to these agents is essential. Multiplex PCR is a simple, sensitive, inexpensive, relatively quick and very specific in addition, it has an ability to identify multiple genes simultaneously.

Key words: Staphylococcus aureus, Panton -Valentine leukocidin, Cna, Multiplex PCR

 

Funding: This research was funded by Saveh Science and Research Branch, Islamic Azad University, Saveh, Iran

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Saveh Science and Research Branch, Islamic Azad University approved the study.

 

How to cite this article: Kord Z, Amini K, Parviz M. Determining the Frequency of Panton-Valentine Leukocidin (PVL), and Collagen- Binding protein (can) in Staphylococcus Aureus Strains Isolated from Clinical Specimens and Antibiotic Resistance: A Short Report. J RafsanjanUniv Med Sci 2015 14(7): 703-10. [Farsi]

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Type of Study: Research | Subject: Microbiology
Received: 2015/04/13 | Accepted: 2015/07/1 | Published: 2015/11/15

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