Volume 15, Issue 4 (7-2016)                   JRUMS 2016, 15(4): 355-376 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sheykhhasan M, Ghiasi M. Evaluation of the Production Methods of Induced Pluripotent Stem Cells: A Short Review. JRUMS 2016; 15 (4) :355-376
URL: http://journal.rums.ac.ir/article-1-3190-en.html
Department of Stem Cell, The Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran.
Full-Text [PDF 1452 kb]   (6367 Downloads)     |   Abstract (HTML)  (5350 Views)
Full-Text:   (9029 Views)

Evaluation of the Production Methods of Induced Pluripotent Stem Cells:

A Short Review

M. Sheykhhasan[1], M. Ghiasi[2]

Received: 02/03/2016      Sent for Revision: 11/04/2016      Received Revised Manuscript: 21/05/2016     Accepted: 24/05/2016

Background and Objectives: Nowadays, cell therapy is one of the most important and promising strategies in the treatment of diseases. Unique capabilities of stem cells caused them to be used in both research and treatment as a valuable resource in basic science and medical researches.

The use of stem cells has been limited due to the related ethical problems. One of the major concerns of scientists to develop and use more of this science is finding alternative methods for producing these cells. Creating induced pluripotent stem cells (IPSCs) is one of the most important methods in achieving this goal. In this regard, various methods are developing that have the ability to change the gene expression and protein profiles, which results in a change in cells morphology and function in the direction of returning to a undifferentiated status and creating stem cells. These methods include nuclear transfer, use of cell extracts and synthetic molecules, mandatory expression of specific genes, and changes in the levels of cytoplasmic. In this review article, it has been tried to review the methods in the field of the induced pluripotent stem cells production.

Literature search was performed through various combinations of keywords regarding induced pluripotent stem cells, Dedifferentiation methods, and IPSCs generation methods. Papers included in our review cover the period between 2002 to 2015.At the end of the review process, 68 articles were used in our final manuscript. In the present study, we review various methods in the IPS cells generation.

Key words: Induced pluripotent stem cells, Dedifferentiation methods, IPSCs generation methods

Funding: This research was funded by The Academic Center for Education, Culture and Research, Qom, Iran.

Conflict of interest: None declared.

Ethical Approval: The Ethics Committee of Academic Center for Education, Culture and Research, Qom, Iran approved the study.

How to cite this article: Sheykhhasan M, Ghiasi M. Evaluation of the Production Methods of Induced Pluripotent Stem Cells :A Short Review. J Rafsanjan Univ Med Sci 2016; 15(4): 355-76. [Farsi]

 

[1]MSc in Biotechnology, Laboratory of Stem Cell, The Academic Center for Education, Culture and Research, Qom, Iran

[2]-MSc in Molecular and Cellular Biology , Laboratory of Stem Cell, The Academic Center for Education, Culture and Research, Qom, Iran

   (Corresponding Author) Tel: (025) 32700155, Fax: (025) 32700154, E-mail: mahdieh.ghiasi@yahoo.com

Type of Study: Review Article | Subject: زيست شناسي
Received: 2016/02/24 | Accepted: 2016/05/24 | Published: 2016/07/18

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Rafsanjan University of Medical Sciences

Designed & Developed by : Yektaweb