Volume 15, Issue 10 (1-2017)                   JRUMS 2017, 15(10): 903-916 | Back to browse issues page

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Baharshahi A, . Keikhosravi A, Behravan M. Identification of Leishmania Parasites and Sandflies Fauna in Zirkouh City, Khorasan-e-Jonoobi Province. JRUMS. 2017; 15 (10) :903-916
URL: http://journal.rums.ac.ir/article-1-3444-en.html
Msc Birjand Hepatitis Research Center & Department of Microbiology, Birjand University of Medical Science, Birjand, Iran
Abstract:   (1460 Views)

Background and Objective: Leishmaniasis is one of the most important vector-borne zoonosis that transmits to human by sand fly species. The current study was carried out to identify sand fly species in Zirkooh, Khorasan-e-Jonobi Province. It was also aimed to determine Leishmania species in infected sand flies by RFLP-PCR method.

Materials and methods:Sand fly specimens were collected by using sticky traps and mechanical aspirator from different parts of Zirkooh city during summer 2015. Captured females were first mounted and then were identified at species level. DNA of the parasite was isolated using extraction kit. The gene ITS-1 was amplified using the parasite specific primers and then RFLP-PCR method was used to identify them.

Results: In this study, a total of 460 specimens were collected, of which 380 sand flies collected from animal facilities and 80 sand flies from residential areas. The most prevalent species, out of 110 females in total, was Phlebotomus sergenti (59.42%) and the second most prevalent was Sergentomyia sintoni (7.25%). Among the examined sandflies, only one specimen belonged to Phlebotomu sergenti was infected with Leishmania tropica; identified by RFLP-PCR.

Conclusion: Considering the presence of Ph. sergenti, as the dominant species in the region, which is component of disease vectors of the urban type of cutaneous leishmaniasis and the presence of Leishmania tropica in the region, the city's health system must take a necessary action for controlling cutaneous leishmaniasis.

Key words: Sand flies, Leishmania, Cutaneous Leishmaniasis, Zirkooh

Funding: This research was funded by Birjand University of Medical Sciences, Iran

Conflict of interest: None declared.

Ethical approval: The Ethical Committee of Birjand University of Medical Sciences, approved the study.

How to cite this article: Baharshahi A, Keikhosravi AR, Behravan M. Identification of Leishmania Parasites and Sandflies Fauna in Zirkouh City, Khorasan-e-Jonoobi Province. J Rafsanjan Univ Med Sci 2017; 15(10): 903-16. [Farsi]

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Type of Study: Research | Subject: Infectious & Tropical Diseases
Received: 2016/09/3 | Accepted: 2016/12/24 | Published: 2017/01/29

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