Volume 18, Issue 2 (5-2019)                   JRUMS 2019, 18(2): 147-160 | Back to browse issues page

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Nazari-serenjeh F, Darbandi N, Yadegary A, Momeni H R. The Effect of Intra-ventral Tegmental Area Injection of Ghrelin on Morphine-Induced Amnesia in Male Rats: An Experimental Study . JRUMS. 2019; 18 (2) :147-160
URL: http://journal.rums.ac.ir/article-1-4445-en.html
Arak University
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The Effect of Intra-ventral Tegmental Area Injection of Ghrelin on Morphine-Induced Amnesia in Male Rats: An Experimental Study
 
 
F. Nazari-serenjeh[1], N. Darbandi[2], A. Yadegary[3], H. R. Momeni[4]
 
Received: 29/09/2018  Sent for Revision: 05/11/2018    Received Revised Manuscript: 19/12/2018              Accepted: 02/01/2019
 
Background and Objectives: Extensive evidence shows that morphine impairs cognitive functions. On the other hand, ghrelin is a gastrointestinal hormone that affects learning and memory process. The purpose of the current study was to evaluate the effect of intra-ventral tegmental area (VTA) injection of ghrelin on morphine-induced amnesia in male rats.
Materials and Methods: In this experimental study, 104 male Wistar rats (220-250 g) were randomly divided into 13 groups (n=8 for all groups) including: saline, morphine treated groups (0.5-7.5 mg/kg), ghrelin treatd groups (0-3 nmol/μl) plus morphine (7.5 mg/kg) or saline. Memory was measured in a step-through type inhibitory avoidance apparatus. All groups received intra-ventral tegmental area (intra-VTA) injection of saline or ghrelin immediately after training. After 5 mins saline or morphine was injected subcutaneously. Testing phase was done 24 hrs after training. Data were analyzed using one-way and two-way ANOVA analysis followed by Tukey’s multiple comparison test.
Results: The results showed that post-training administration of morphine significantly reduced step-through latency compared with the saline group (p<0.001). Intra-VTA injection of ghrelin also reduced morphine-induced amnesia (7.5 mg/kg) (p< 0.001). Ghrelin plus saline administration had no significant effect on memory retrieval (p>0.05).
Conclusion: The results indicate that intra-VTA injction of ghrelin prevents the disrupting effects of morphine on avoidance memory.
Key words: Ghrelin, Inhibitory avoidance learning, Male rat, Morphine, Ventral tegmentum area
Funding: This study was funded by University of Arak.                             
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Arak University of Medical Sciences approved the study. (1397.139).
 
How to cite this article: Nazari-serenjeh F, Darbandi N, Yadegary A, Momeni HR. The Effect of Intra-ventral Tegmental Area Injection of Ghrelin on Morphine-Induced Amnesia in Male Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2019; 18 (2): 147-60. [Farsi]
 
 
 
  1. - Assistant Prof., Dept. of Biology, Payam-e-Noor University (PNU), Tehran, Iran, ORCID: 0000-0001-7278-3655
  1. - Assistant Prof., Dept. of Biology, Faculty of Science, Arak University, Arak, Iran, ORCID: 0000-0001-8886-8745
(Corresponding Author): Tel: (086) 32627224, Fax: (086) 32627543, E-mail: N-Darbandi@araku.ac.ir
[3]- MSc Student of Animal Physiology, Dept. of Biology, Faculty of Science, Arak University, Arak, Iran, ORCID: 0000-0001-8593-386X
[4]- Associate Prof., Dept. of Biology, Faculty of Science, Arak University, Arak, Iran, ORCID: 0000-0002-1361-5771
Type of Study: Research | Subject: Physiology
Received: 2018/09/23 | Accepted: 2019/01/2

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