Volume 10, Issue 2 (7-2011)
JRUMS 2011, 10(2): 112-126 |
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The Effect of Honey Bee Venom and All-trans Retinoic Acid on Proliferation and Differentiation of HL-60 Cell Line. JRUMS 2011; 10 (2) :112-126
URL: http://journal.rums.ac.ir/article-1-5322-en.html
URL: http://journal.rums.ac.ir/article-1-5322-en.html
Abstract: (1652 Views)
Background and Objectives: Differentiation therapy is one of the methods for treatment of cancer. In this method, some drugs such as All-trans Retinoic Acid, are used which can inhibit proliferation of cancerous cells and induce cell differentiation. Previus experiments showed some anti-proliferation materials, anti-inflammation materials or anti-oxidant materials could influence the effects of such drugs and decrease resulting side effects. In this research the effects of the Honey Bee Venom, on All-trans Retinoic Acid functions was measured.
Materials and Methods: In this experimental project we used HL-60 cell line belonging to acute promyelocyte leukemia. The HL-60 cell line was obtained from Pasteur Institute of Iran, and were grown in RPMI 1640 medium (Roswell Park Memorial Institute) containing 10% FBS (Fetal Bovine Serum) and 1% streptomycin-penicilin. We used MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) and NBT (Nitro-Blue tetrazolium chloride) tests for these purposes. All experiments were done three times and data were analyzed using SPSS.
Results: Both Honey Bee Venom and All-trans Retinoic Acid could inhibit proliferation of HL-60 cells, also All-trans Retinoic Acid could induce differentiation in this cells. The amount of differentiation was increased significantly (p<0.01) in the presence of Honey Bee Venom.
Conclusion: On the base of our findings, it seemed that Honey Bee Venom could increase anti cancer effects of All-trans Retinoic Acid.
Key words: Honey Bee Venom, HL-60 cell line, Cell Proliferation and Differentiation, All-trans Retinoic Acid
Funding: This project was funded by Tarbiat Moallem University of Tehran.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Tarbiat Moallem University approved the study.
How to cite this article: Parivar K, Nabiuni M, Jalali H, Nadali F. The Effect of Honey Bee Venom and All-trans Retinoic Acid on Proliferation and Differentiation of HL-60 Cell Line. J Rafsanjan Univ Med Sci 2011; 10(2): 112-26. [Farsi]
Materials and Methods: In this experimental project we used HL-60 cell line belonging to acute promyelocyte leukemia. The HL-60 cell line was obtained from Pasteur Institute of Iran, and were grown in RPMI 1640 medium (Roswell Park Memorial Institute) containing 10% FBS (Fetal Bovine Serum) and 1% streptomycin-penicilin. We used MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) and NBT (Nitro-Blue tetrazolium chloride) tests for these purposes. All experiments were done three times and data were analyzed using SPSS.
Results: Both Honey Bee Venom and All-trans Retinoic Acid could inhibit proliferation of HL-60 cells, also All-trans Retinoic Acid could induce differentiation in this cells. The amount of differentiation was increased significantly (p<0.01) in the presence of Honey Bee Venom.
Conclusion: On the base of our findings, it seemed that Honey Bee Venom could increase anti cancer effects of All-trans Retinoic Acid.
Key words: Honey Bee Venom, HL-60 cell line, Cell Proliferation and Differentiation, All-trans Retinoic Acid
Funding: This project was funded by Tarbiat Moallem University of Tehran.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Tarbiat Moallem University approved the study.
How to cite this article: Parivar K, Nabiuni M, Jalali H, Nadali F. The Effect of Honey Bee Venom and All-trans Retinoic Acid on Proliferation and Differentiation of HL-60 Cell Line. J Rafsanjan Univ Med Sci 2011; 10(2): 112-26. [Farsi]
Keywords: Honey Bee Venom, HL-60 cell line, Cell Proliferation and Differentiation, All-trans Retinoic Acid
Type of Study: Research |
Subject:
زيست شناسي
Received: 2020/05/11 | Accepted: 2020/05/11 | Published: 2020/05/11
Received: 2020/05/11 | Accepted: 2020/05/11 | Published: 2020/05/11
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