Volume 2, Number 1 (6-2003)                   JRUMS 2003, 2(1): 1-9 | Back to browse issues page


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Abdollahi S. Preparation, Purification and Identification of Excreted/Secreted Antigens of Toxoplasma Gondii Tachyzoites. JRUMS. 2003; 2 (1) :1-9
URL: http://journal.rums.ac.ir/article-1-56-en.html

استاديار گروه ميكروبيولوژي
Abstract:   (15239 Views)

 Preparation, Purification and Identification of Excreted/Secreted Antigens of Toxoplasma Gondii Tachyzoites

 

  S.H. Abdollahi1 *

 

 1- Assistant Professor, Department of Microbiology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

 

  Background: The Excreted/Secreted antigens (E/SA) of toxoplasma gondii tachyzoites are thought to play an important role in the pathogenesis of parasite and the hosts, immunity against infection with this Parasite . These antigens are also useful for diagnosis and differentiation of acute from chronic phase of toxoplasmosis . Therefore the aim of the present study was to detect the best technique for preparation, purification and identification of these antigens.

  Materials and Methods: Tachyzoites (2×108) were incubated at 37°C for 1hour under mild agitation in test–tubes containing 2ml of RPMI-1640 culture medium without FCS. After centrifugation for 15min at 1000g the E/SA containing supernatant were dialyzed and concentrated. E/SA were separated by gel filtration and ion exchange chromatography.

  Fractions of both chromatography columns were analyzed by Native–PAGE electropho_ resis.

  Results: E/SA were eluted in three and two Peaks by gel filtration and ion-exchange chromatography respectively. The second peak obtained from ion-exchange chromatograp_ hy produced one band in native – PAGE.

  Conclusion: The result of this experiment showed that ion-exchange chromatography under this conditions is a good tool for purification of E/SA .

 

  Keywords: Toxoplasma Gondii, Excreted/Secreted Antigens, Ion-exchange Choromatography, Electrophoresis

 

  * Corresponding author tel: (391) 5234003-5

Journal of Rafsanjan University of Medical Sciences and Health Services, 2002, 2(1): 1-9

 

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Type of Study: Research | Subject: Microbiology
Received: 2005/12/8

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