TY - JOUR T1 - Comparison of in vitro Cytotoxic Effect of Two MTAs (Iran Made and Imported) on Peripheral Blood Mononuclear Cells TT - مقایسه آزمایشگاهی سمیت سلولی دو نوعMTA (ایرانی Ùˆ خارجی) بر روی سلول‌های تک هسته‌ای خون محیطی JF - RUMS_JOURNAL JO - RUMS_JOURNAL VL - 4 IS - 4 UR - http://journal.rums.ac.ir/article-1-124-en.html Y1 - 2005 SP - 320 EP - 325 KW - Root MTA KW - MTA KW - Cell cytotoxicity KW - MTT assay N2 -   Comparison of in vitro Cytotoxic Effect of Two MTAs (Iran Made and Imported) on Peripheral Blood Mononuclear Cells   Â  K. Nazari Mogaddam DDS [1] , T. Ghazanfari PhD [2] , M. Mohammadi Basir DDS [3] , M. Emadi [4]   Â  Background and Objective: Imported mineral Trioxide Aggregate (MTA) as a root –end filling material has many applications in root repairing and bone healing including direct pulp capping, repair of root and furcation perforation, and apexification. By introducing a similar material, made in Iran, namely Root MTA, primary tests such as cytotoxicity test should be performed. The purpose of this study was to compare the 24h and 48h cytotoxicity effect of Root MTA on peripheral blood mononuclear cells using MTT Assay.   Materials and Methods : In this study, after blood sampling, peripheral blood mononuclear cells were isolated on a gradient of ficole hypac. After several washing phases, cells were cultured in RPMI with 10% FCS in 96 well U shape microtiterplates in a 5% Co2 incubator. Serial dilution of MTA and Root MTA were added to the wells. Cell cytotoxicity was determined, 24 and 48 hours after treatment by MTT Assay.   Results : Cytotoxicity of Root MTA was less than that of MTA (ProRoot ). At 24 hours after culture there was significant difference between cell viability affected by Root MTA and MTA in the three different dilutions 20 λ , 50 λ , and 100 λ (p M3 ER -