fa تأثیر تمرین تناوبی با شدت بالا همراه با مصرف ویتامین E بر سطوح سرمی‌آیریزین و بیان ژن پروتئین غشایی FNDC5عضله سولئوس در موش‌های صحرایی نر نژاد ویستار تربيت بدني تربيت بدني پژوهشي Research <div><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چکیده</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زمینه و هدف</span></span></strong>: <span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">فعالیت بدنی با تأثیر بر هورمون آیریزین سبب تغییر ساختار بافت چربی سفید و تبدیل آن به چربی قهوه&shy;ای می‌شود که می‌تواند مشکلات مربوط به چاقی را کاهش دهد</span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">.</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> ﻫﺪف ﭘﮋوﻫﺶ حاضر تعیین تأثیر </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تمرین تناوبی با شدت بالا و</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مصرف ویتامین</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">E</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> &nbsp;برسطوح آیریزین و بیان ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FNDC5</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (</span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">fibronectin type III domain containing 5</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در موش‌های صحرایی بود.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مواد و روش&shy;ها:</span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> در این مطالعه تجربی32 ﺳﺮ </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موش صحرایی </span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">نر</span></span></span> <span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">ﻧﮋاد ویﺴﺘﺎر (</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">200-150 گرم) به طور تصادفی به 4 گروه </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﮐﻨﺘﺮل، ﻣﮑﻤﻞ، ﺗﻤرین، ﺗﻤﺮیﻦ+ﻣﮑﻤﻞ) تقسیم شدند. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">برنامه تمرینی شامل هشت هفته، هفته‌ای پنج جلسه، هر جلسه 15 دقیقه شامل 5 دقیقه تمرین برای گرم کردن، 4 دقیقه تناوب شدید، 2 دقیقه تناوب بازیافت و 3 دقیقه سرد کردن بود.</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مقدار 100 میلی‌گرم بر کیلوگرم ویتامین </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">E</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در گروه‌های مکمل به مدت 8 هفته، سه بار در هفته به صورت داخل صفاقی تجویز شد.</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 48</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ﺳﺎﻋﺖ ﭘﺲ از ﭘﺎیﺎن آﺧﺮیﻦ ﺟﻠﺴﻪ ﺗﻤﺮیﻦ، نمونه خون از حیوانات ﮔﺮﻓﺘﻪ ﺷﺪ و ﺳﻄﻮح </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آیریزین </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> روش الایزا و هم‌چنین بیان ژن</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FNDC5 </span></span>&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با روش </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">RT-PCR</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> اﻧﺪازهﮔﯿﺮی ﺷﺪ. جهت آنالیز داده‌ها از روش آﻣﺎری ﺗﺤﻠﯿﻞ واریﺎﻧﺲ یﮏﻃﺮﻓﻪ </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و دوطرفه استفاده شد.</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نتایج نشان داد 8 هفته</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تمرین تناوبی با شدت بالا همراه با مصرف ویتامین</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"> E</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سبب افزایش معنی‌دار ﺳﻄﻮح </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آیریزین و</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FNDC5 </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;در مقایسه با گروه کنترل می‌شود (001/.></span></span><span dir="LTR"><span style="font-family:times new roman,serif;">P</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) .</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجه‌گیری:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تمرین تناوبی با شدت بالا همراه با مصرف ویتامین</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"> E</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">احتمالاً محرک ترشح مایوکاین&shy;هایی مانند آیریزین است که می‌تواند بافت چربی سفید را تحت تأثیر قرار دهد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">.</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">واژه‌های کلیدی:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ویتامین </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">E</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آیریزین،</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FNDC5 </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موش صحرایی، تمرین تناوبی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></div> <br clear="all" > &nbsp; &nbsp; <pre> <strong>Background and Objectives:</strong> Physical activity with the effect on the irisin hormone alters the structure of the white adipose tissue and turns it into brown fat that can reduce obesity related complications. This study aimed at determining the effect of high intensity interval training<span dir="RTL">) </span>HIIT) and vitamin E consumption on levels of irisin and gene expression of FNDC5 (fibronectin type III domain containing 5) in Wistar rats.<strong><span dir="RTL"></span></strong></pre> <strong>Materials and Methods:</strong> In this experimental study, 32 male Wistar rats (150-200 grams) were randomly divided into four groups: control, supplement, training, training + supplement. Training program was planned for 8 weeks, 5 sessions per week, each session 15 minutes: 5 minutes warm up, 4 minutes intensive interval training, 2 minutes recycling, and 3 minutes cooling down. The amount of 100 mg/kg vitamin E was administered intraperitoneally in the supplement groups for 8 weeks and three times per week. Blood samples were taken 48 hours after the last training session and the levels of irisin were measured by using ELISA and FNDC5 gene expression by RT-PCR method. One-way and two-way analysis of variance were used for data analysis.<br> <strong>Results:</strong> The results showed that in comparison with the control group, eight weeks of HIIT with vitamin E consumption caused a significant increase in the levels of irisin and FNDC5 (p<0.001).<br> <strong>Conclusions:</strong> HIIT with vitamin E consumption through secretion of myokins like irisin may affect white adipose tissue.<br> <strong>Key words:</strong> Vitamin E, Irisin, FNDC5, Rat, Interval training<br> &nbsp;<br> <strong>Funding: </strong>This study did not have any funds.<br> <strong>Conflict of interest: </strong>None declared.<br> <strong>Ethical approval: </strong>The Ethics Committee of Islamic Azad University, &nbsp;East Tehran Branch approved the study (Ethics number#139427).<br> &nbsp;<br> <strong>How to cite this article: </strong>Hosseini M, Fatollah Zadeh P. The Effect of High Intensity Interval Training with Vitamin E Consumption on Serum Levels of Irisin and Gene Expression of Membrane Protein FNDC5 of Soleus Muscle in Male Wistar Rats. <em>J Rafsanjan Univ Med Sci</em> 2018; 17 (7): 611-24. [Farsi]<br> &nbsp; <pre> </pre> ویتامین E, آیریزین,FNDC5 , موش صحرایی, تمرین تناوبی Vitamin E, Irisin, FNDC5, Rat, Interval training 611 624 http://journal.rums.ac.ir/browse.php?a_code=A-10-3083-4&slc_lang=fa&sid=1 M. Hosseini معصومه حسینی mhbisadi@yahoo.com 6900319475328460030949 6900319475328460030949 Yes Islamic Azad univeTehrarsity east Tehran branch دانشگاه آزاد تهران شرق P. Fatollah Zadeh پریسا فتح الله زاده parisa.fatolah@gmail.com 6900319475328460030950 6900319475328460030950 No Islamic Azad univeTehrarsity east Tehran branch دانشگاه آزاد تهران شرق