fa نقش محافظتی عصاره آبی سورانه (Allium saralicum) بر قند خون و برخی فاکتورهای بیوشیمیایی مرتبط در موش‌های صحرایی نر دیابتی القاء شده با آلوکسان: یک مطالعه تجربی فيزيولوژي Physiology پژوهشي Research <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زمینه و هدف: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دیابت یکی از شایع&shy;ترین اختلالات متابولیکی می&shy;باشد. گیاهان دارویی برای کنترل دیابت استفاده می&shy;شوند. هدف از مطالعه حاضر تعیین اثر محافظتی سورانه بر قند خون و برخی فاکتورهای بیوشیمیایی مرتبط در دیابت نوع یک القاء شده با آلوکسان در موش&shy;های صحرایی نر بود. </span></span><b><i></i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مواد و روش</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;</span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها:</span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی، دیابت با تزریق یک دوز داخل صفاقی آلوکسان (120 میلی&shy;گرم/کیلوگرم) القاء شد. تعداد&nbsp; 30 سر موش صحرایی نر </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در محدوده وزنی 20</span></span><span lang="AR-SA" style="font-size:12.0pt">&plusmn;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">200 گرم </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به 6 گروه 5 تایی تقسیم شدند. گروه 1 (کنترل نرمال)، گروه 2 (کنترل دیابتی)، گروه 3 (گروه دیابتی دریافت کننده گلی بنکلامید با دوز 20 میلی&shy;گرم/کیلوگرم)، گروه&shy;های 4 تا 6 شامل گروه&shy;های دیابتی که به ترتیب عصاره آبی سورانه با دوزهای 20، 60 و 180 میلی&shy;گرم/کیلوگرم را به صورت خوراکی و به مدت 25 روز دریافت کردند. در پایان دوره درمانی، نمونه&shy;های خون جمع&shy;آوری و سطوح سرمی گلوکز، </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلکالین فسفاتاز، آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لاکتات دهیدروژناز</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اوره و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کراتینین ارزیابی شدند. داده&shy;ها توسط آنالیز واریانس </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یک</span></span>‌<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">طرفه </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و آزمون تعقیبی </span></span>Duncan<span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تجزیه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;تحلیل شدند.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یافته&shy;ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج نشان داد عصاره سورانه </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کاهش وزن بدن را در گروه دیابتی بهبود بخشید </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(05/0</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">></span></span>p<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). هم&shy;چنین، سطوح سرمی گلوکز، </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آلکالین فسفاتاز، آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لاکتات دهیدروژناز</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> اوره و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کراتینین </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در گروه دیابتی بالاتر از گروه کنترل بود (05/0</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">></span></span>p<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این تغییرات با درمان با عصاره سورانه در دوزهای مختلف مشابه گلی بنکلامید بهبود یافت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(05/0</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">></span></span>p<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه&shy;گیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عصاره سورانه ممکن است اثر محافظتی در برابر تغییرات قند خون و برخی فاکتورهای بیوشیمیایی ناشی از دیابت القاء شده با آلوکسان داشته باشد.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">واژه&shy;های کلیدی: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عصاره آبی سورانه، آلوکسان، دیابت، موش صحرایی</span></span><span dir="LTR" style="font-size:19.0pt"></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span dir="RTL" lang="AR-SA" style="font-size:5.0pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Background and Objectives:</span></b><span style="line-height:140%"> Diabetes is one of the most common metabolic disorders. Medicinal plants have been used for controlling diabetes. The aim of the present study was to investigate the protective effect of <i>Allium saralicum </i>on blood glucose and some related biochemical factors in alloxan induced type-1 diabetes in male rats. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Materials and Methods: </span></b><span style="line-height:140%">In this experimental study,<b> </b>diabetes was induced by single-dose intraperitoneal injection of alloxan (120 mg/kg/bw). Thirty male rats weighing 200&plusmn;20 g were divided into 6 groups of 5. Group 1 (normal control), Group 2 (diabetic control), Group 3 (diabetic group receiving glibenclamide (20 mg/kg/bw), Groups 4 to 6 including diabetic groups receiving aqueous extract of <i>Allium saralicum</i> (20, 60 and 180 mg/kg/bw), respectively by gavage for 25 days. At the end of the treatment period, blood samples were collected and serum levels of glucose, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, urea, and creatinine were evaluated. Data were analyzed using one-way analysis of variance and Duncan&rsquo;s post hoc tests. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Results:</span></b><span style="line-height:140%"> The results indicated that <i>Allium saralicum </i>extract improved body weight loss in the diabetic group (p<0.05). Also, serum levels of glucose, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, urea, and creatinine in the diabetic group were higher than those in the control group (p<0.05). These changes were ameliorated by treatment with <i>Allium saralicum </i>extract at different doses similar to glibenclamide (p<0.05).</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Conclusion:</span></b><span style="line-height:140%"> <i>Allium saralicum </i>extract may have a protective effect against blood glucose and some biochemical factors<b> </b>alterations caused by alloxan-induced diabetes.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Key words:</span></b><span style="line-height:140%"> <i>Allium saralicum </i>Aqueous Extract,<i> </i>Alloxan, Diabetes, Rat</span><b><span dir="RTL" lang="AR-SA" style="font-size:3.0pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Funding: </span></b><span style="line-height:140%">This study was funded by Razi University, Kermanshah, Iran.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Conflict of interest:</span></b><span style="line-height:140%"> None declared.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">Ethical approval: </span></b><span style="line-height:140%">The Ethics Committee of Razi University approved the study (Ethic Nubmer: 396</span><span style="line-height:140%"><span calibri="" light="" style="font-family:">-2-037).</span></span><b><span dir="RTL" lang="AR-SA" style="font-size:2.0pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">How to cite this article:</span></b><b> </b>Bahrami Erfan, Pooyanmehr Mehrdad, Maleki Ali, Alimohammadi Samad<span style="font-size:14.0pt"><span style="line-height:150%">.</span></span><span style="line-height:150%">The Protective Effects of <i>Allium Saralicum</i> Aqueous Extract on Blood Glucose and Some Related Biochemical<b> </b>Factors in Alloxan-Induced Diabetic Male Rats: An Experimental Study.<i> J Rafsanjan</i></span><span style="line-height:150%"> <i>Univ Med Sci</i> 2022; 21 (07): 728</span><span style="line-height:150%"><span calibri="" light="" style="font-family:">-40.</span></span><span style="line-height:150%"> [Farsi]</span><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br> &nbsp; عصاره آبی سورانه, آلوکسان, دیابت, موش صحرایی Allium saralicum Aqueous Extract, Alloxan, Diabetes, Rat 729 740 http://journal.rums.ac.ir/browse.php?a_code=A-10-4606-2&slc_lang=fa&sid=1 Erfan Bahrami عرفان بهرامی drerfanvet@gmail.com 6900319475328460051588 6900319475328460051588 No Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران. Mehrdad Pooyanmehr مهرداد پویان مهر M.pooyanmehr@razi.ac.ir 6900319475328460051589 6900319475328460051589 No Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران. Ali Maleki علی ملکی ali.maleki@kums.ac.ir 6900319475328460051590 6900319475328460051590 No Kermanshah University of Medical Sciences, Kermanshah, Iran. دانشگاه علوم پزشکی کرمانشاه، کرمانشاه ، ایران. Samad Alimohammadi صمد علی محمدی S.alimohammadi@razi.ac.ir 6900319475328460051591 6900319475328460051591 Yes Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran. دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.