Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1401
7
1
gregorian
2022
10
1
21
7
online
1
fulltext
fa
تأثیر مصرف سرکه سیب بر شاخصهای عملکرد کبدی در موشهای صحرایی نر مبتلا به دیابت نوع 1: یک مطالعه تجربی
The Effect of Apple Cider Vinegar Consumption on Liver Function Indices in Male Rats with Type 1 Diabetes: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">چکیده</span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">دیابت از شایع­ترین اختلالات متابولیسمی با آثار سوء بر کبد میباشد. مطالعه در زمینه یافتن عوامل مفید بر عملکرد کبد در بیماری دیابت حائز اهمیت است. هدف مطالعه حاضر، تعیین اثرات سرکه سیب بر شاخصهای کبدی موشهای صحرائی دیابتی بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> در این مطالعه تجربی، 28 سر موش صحرایی نر با میانگین وزنی 200 گرم به 4 گروه شامل کنترل، دیابتی، دیابتی دریافتکننده سرکه سیب 5/0 درصد یا 5 درصد تقسیم شدند. محلول­ها با حجم 4 میلی­لیتر/کیلوگرم به­مدت شش هفته به­صورت خوراکی تجویز گردید. دیابت با تزریق درونصفاقی 50 میلی­گرم/کیلوگرم استرپتوزوتوسین القاء شد. سرکه سیب با روش سنتی از </span></span></span><i>Malus domestica</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> تهیه و به­صورت تازه استفاده شد. در پایان، سطوح سرمی کلسترول، تریگلیسرید، </span></span></span><i>low-density lipoprotein</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (</span></span></span><i>LDL</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">)، </span></span></span><i>high-density lipoprotein</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (</span></span></span><i>HDL</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">)، پروتئینتام، آلبومین و آنزیمهای </span></span></span><i>aspartate transaminase</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (</span></span></span><i>AST</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">)، </span></span></span><i>alanine aminotransferase</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (</span></span></span><i>ALT</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">)، </span></span></span><i>alkaline phosphatase</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (</span></span></span><i>ALP</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) و بیلیروبین اندازهگیری شدند. بافت کبد بهروش هماتوکسیلین-ائوزین مطالعه گردید. دادهها با آنالیز واریانس یکطرفه و پسآزمون </span></span></span><i>Tukey</i><span style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> تجزیه­ و تحلیل شدند.</span></span></span><i></i></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> در موشهای مبتلا به دیابت در مقایسه با موشهای سالم به­طور معنیداری سطوح قند خون، کلسترول، تریگلیسرید، </span></span></span><i>LDL</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، </span></span></span><i>AST</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، </span></span></span><i>ALT</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، </span></span></span><i>ALP</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> و بیلیروبین سرم و فیبروز، سیروز، دژنراسیون و التهاب در بافت کبد افزایش (05/0</span></span></span><i>p<</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) و وزن بدن، </span></span></span><i>HDL</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، پروتئین تام و آلبومین سرم کاهش یافت (05/0</span></span></span><i>p<</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">). سرکه سیب 5/0 درصد یا 5 درصد این شاخص­ها را تعدیل و اختلاف معنیداری با گروه دیابتی ایجاد کرد (05/0</span></span></span><i>p<</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> سرکه سیب با کنترل قند خون و پروفایل لیپیدی باعث تعدیل عوارض کبدی ناشی از دیابت میگردد.</span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""> دیابت، سرکه سیب، پروفایل لیپیدی، آنزیمهای کبدی، موش صحرایی</span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Background and Objectives:</span></b><span style="line-height:140%"> Diabetes is one of the most common metabolic disorders with adverse effects on the liver.</span> <span style="line-height:140%"> It is important to study the factors that improve the liver function in diabetes. The present study aimed at evaluating the effects of apple-vinegar on the liver indices of diabetic rats.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Materials and Methods:</span></b><span style="line-height:140%"> In this experimental study, 28 male <i>Wistar</i> rats with an average weight of 200g were divided into 4 groups including control, diabetic, and diabetic groups receiving 0.5% or 5% apple-vinegar. Solutions with a volume of 4 ml/kg were administered orally for six weeks. Diabetes was induced by intraperitoneal injection of 50mg/kg streptozotocin. Apple-vinegar was prepared by traditional method from <i>Malus-domestica</i> and used fresh. At the end of treatment, serum levels of cholesterol, triglyceride, low-density lipoprotein (LDL), high-density lipoprotein (HDL), total protein, albumin and aspartate transaminase (AST),</span> <span style="line-height:140%">alanine aminotransferase (ALT), alkaline phosphatase</span> <span style="line-height:140%">(ALP), and bilirubin were measured. Liver tissue was studied by Hematoxylin-Eosin method. Data were analyzed by one-way analysis of variance and Tukey's post-test.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Results:</span></b><span style="line-height:140%"> In the diabetic rats compared to the healthy rats, the levels of blood glucose, cholesterol, triglyceride, LDL, AST, ALT, ALP, bilirubin serum, and fibrosis, cirrhosis, degeneration and inflammation of the liver tissue significantly increased (p<0.05), and the body weight, serum HDL, total protein, and albumin significantly decreased (p<0.05). Five or 0.5% apple-vinegar modulated these indices, and in most cases, stablished a significant difference with the diabetic group (p<0.05). </span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Conclusion:</span></b><span style="line-height:140%"> Apple-vinegar modulated liver complications due to diabetes by regulating blood glucose and lipid profile.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Key words:</span></b><span style="line-height:140%"> Diabetes, Apple vinegar, Lipid profile, Liver enzymes, Rat</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Funding:</span></b><span style="line-height:140%"> This study was funded by Urmia University. </span><span lang="FA" dir="RTL" style="line-height:140%"></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Conflict of interest:</span></b><span style="line-height:140%"> None declared.</span><span lang="FA" dir="RTL" style="line-height:140%"></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:140%">Ethical approval:</span></b><span style="line-height:140%"> The Ethics Committee of Urmia University approved this study (IR-UU-AEC 1378/DP/3).</span><span dir="RTL" lang="FA" style="font-size:5.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span style="font-size:1.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span style="font-size:1.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span style="font-size:1.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span dir="RTL" lang="AR-SA" style="font-size:2.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:150%">How to cite this article:</span></b> <span style="line-height:150%">Poursamileh Narges, Babaei-Balderlou Farrin. The Effect of Apple Cider Vinegar Consumption on Liver Function Indices in Male Rats with Type 1 Diabetes: An Experimental Study.<b> </b><i>J Rafsanjan</i></span><span style="line-height:150%"> <i>Univ Med Sci</i> 2022; 21 (7): 699</span><span style="line-height:150%"><span style="font-family:"Calibri Light","sans-serif"">-714</span></span><span style="line-height:150%">. [Farsi]</span><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
دیابت, سرکه سیب, پروفایل لیپیدی, آنزیمهای کبدی, موش صحرایی
Diabetes, Apple vinegar, Lipid profile, Liver enzymes, Rat
699
714
http://journal.rums.ac.ir/browse.php?a_code=A-10-5944-1&slc_lang=fa&sid=1
Narges
Poursamileh
نرگس
پورسمیله
narges.porsamileh@gmail.com
6900319475328460051582
6900319475328460051582
No
Urmia University
دانشگاه ارومیه
Farrin
Babaei-Balderlou
فرین
بابائی بالدرلو
f.babaei@urmia.ac.ir
6900319475328460051583
6900319475328460051583
Yes
Urmia university
دانشگاه ارومیه