fa تعیین محتوای فنول، فلاونوئید کل و ظرفیت آنتی‌اکسیدانی عصاره متانولی سه مرحله رشدی گیاه خارخاسک (Tribulus terrestris L.): یک گزارش کوتاه زيست شناسي زيست شناسي پژوهشي Research <span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><a name="_Hlk113374001"><b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">گیاه خارخاسک</span></span></span><i> </i><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">از گیاهان با خواص دارویی است. هدف مطالعه حاضر تعیین میزان فنول، فلاونوئید کل و فعالیت آنتی‌اکسیدانی مراحل مختلف رشدی اندام‌های هوایی دو جمعیت گیاه خارخاسک بود.</span></span></span><i><span dir="LTR" style="font-size:11.0pt"><span style="line-height:122%"></span></span></i></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">مواد و روش‌ها:</span></span></span></b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"> مطالعه آزمایشگاهی حاضر، در سال 1400 در دو رویشگاه‌ سردسیر و گرمسیر استان ایلام انجام شد. مقدار فنول کل، محتوای فلاونوئیدی کل و فعالیت آنتی‌اکسیدانی عصاره بخش‌های هوایی گیاه اندازه‌گیری و به‌صورت </span></span></span><i><span dir="LTR" style="font-size:11.0pt"><span style="line-height:122%">IC₅₀</span></span></i><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"> بیان گردید. داده&shy;ها با استفاده از آنالیز واریانس یک&shy;طرفه تجزیه و تحلیل شد.</span></span></span><i><span dir="LTR" style="font-size:11.0pt"><span style="line-height:122%"></span></span></i></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">یافته‌ها:</span></span></span></b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"> بیشترین و کمترین محتوای فنول و فلاونوئید کل در مرحله گلدهی رویشگاه گرمسیر و مرحله ظهور ساقه منطقه سردسیر به‌دست آمد. بیشترین و کمترین فعالیت آنتی‌اکسیدانی، به ترتیب مربوط به مرحله گلدهی رویشگاه مهران و مرحله ظهور ساقه رویشگاه آسمان‌آباد بود.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">نتیجه‌گیری:</span></span></span></b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"> مرحله گلدهی رویشگاه گرمسیری مهران دارای بالاترین محتوای فنول کل، فلاونوئید کل و میزان فعالیت آنتی‌اکسیدانی بود. </span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span lang="AR-SA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;">واژه‌های کلیدی:</span></span></span></b><span lang="FA" style="font-size:13.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"> خارخاسک، ظرفیت آنتی‌اکسیدانی، عصاره، ترکیبات فنولی، استان ایلام</span></span></span><span lang="AR-SA" style="font-size:2.0pt"><span style="line-height:122%"><span style="font-family:&quot;B Nazanin&quot;"></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Background and Objectives:</span></b><span style="line-height:150%"> <i>Tribulus</i> <i>terrestris</i> is one of the medicinal plants. The aim of this study was to determine the amount of total phenol and flavonoid, and antioxidant activity in different developmental stages of the aerial parts of two populations of <i>T. terrestris</i>.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Materials and Methods:</span></b><span style="line-height:150%"> The present laboratory study was conducted in 2021 in two habitats, warm and cold climate, in Ilam Province. The amount of total phenol, the total flavonoid content, and the antioxidant activity of the metanolic extract of the aerial parts of the plants were measured and expressed as IC₅₀ (The half-maximal inhibitory concentration). Data were analyzed using one-way analysis of variance.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Results:</span></b><span style="line-height:150%"> The highest and the lowest total phenol and flavonoid contents were obtained respectively in the flowering stage of warmer climate and the stem emergence stage of colder region. The highest and the lowest antioxidant activity were respectively related to the flowering and the stem emergence stages of Mehran and Asmanabad habitats. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Conclusion:</span></b><span style="line-height:150%"> The flowering stage of Mehran tropical habitat had the highest content of total phenol, total flavonoid, and antioxidant activity.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Key words:</span></b><span style="line-height:150%"> Tribulus terrestris<i>,</i> Antioxidant capacity, Extract, Phenolic compounds, Ilam province</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="line-height:150%"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Funding:</span></b><span style="line-height:150%"> This study did not have any funds.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">Conflict of interest:</span></b><span style="line-height:150%"> None declared.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><a><b><span style="line-height:150%">Ethical </span></b></a><span dir="RTL" lang="FA" style="font-size:8.0pt"><span style="line-height:150%"><a class="msocomanchor" href="#_msocom_1" id="_anchor_1" language="JavaScript" name="_msoanchor_1" on<illegal tag>mouseout="msoCommentHide('_com_1')" on<illegal tag>mouseover="msoCommentShow('_anchor_1','_com_1')">[a1]</a>&nbsp;</span></span><b><span style="line-height:150%">approval:</span></b><span style="line-height:150%"> Not applicable.</span><b><span lang="FA" dir="RTL" style="line-height:150%"></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="line-height:150%">How to cite this article:</span></b><b> </b><span style="font-size:9.0pt"><span style="line-height:150%">Baghaeifar Zahra, Darvishnia Hamid, Tamri Jafar, Saeedian Shahriar. Determination of Total Phenolic and Flavonoid Contents and Antioxidant Capacity of Methanolic Extract of <i>Tribulus Terrestris</i> at Three Developmental Stages: A Short Report.<b> </b><i>J Rafsanjan</i> <i>Univ Med Sci</i> 2023; 22 (4): 419-28. [Farsi] </span></span></span></span></span></span></span> <div> <hr align="left" class="msocomoff" size="1" width="33%" > <div> <div class="msocomtxt" id="_com_1" language="JavaScript" on<illegal tag>mouseout="msoCommentHide('_com_1')" on<illegal tag>mouseover="msoCommentShow('_anchor_1','_com_1')"><a name="_msocom_1"></a><span style="font-size:10pt"><span style="line-height:107%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Garamond,serif"><span dir="LTR" style="font-size:8.0pt"><span style="line-height:107%">&nbsp;<a class="msocomoff" href="#_msoanchor_1">[a1]</a></span></span><span lang="FA" style="font-family:&quot;Times New Roman&quot;,serif">کد اخلاق ذکر گردد.</span></span></span></span></span></span></div> </div> </div> خارخاسک, ظرفیت آنتی‌اکسیدانی, عصاره, ترکیبات فنولی, استان ایلام Tribulus terrestris, Antioxidant capacity, Extract, Phenolic compounds, Ilam province 419 428 http://journal.rums.ac.ir/browse.php?a_code=A-10-6107-1&slc_lang=fa&sid=1 Zahra Baghaeifar زهرا بقایی فر z_baghaeifar@pnu.ac.ir 6900319475328460054332 6900319475328460054332 No Payame Noor University دانشگاه پیام نور Hamid Darvishnia حمید درویش نیا darvishnia_h@pnu.ac.ir 6900319475328460054333 6900319475328460054333 Yes Payame Noor University دانشگاه پیام نور Jafar Tamri جعفر تمری jafartamry1362@gmail.com 6900319475328460054334 6900319475328460054334 No Payame Noor University دانشگاه پیام نور Shahriar Saeedian شهریار سعیدیان saeedyan@pnu.ac.ir 6900319475328460054335 6900319475328460054335 No Payame Noor University دانشگاه پیام نور