fa اثرات ضد باکتری عصاره‌های الکلی گیاهان میخک، رزماری، چای ترش، چای سبز و پونه کوهی بر تعدادی از باکتری‌های حفره دهان: یک مطالعه آزمایشگاهی ميكروبيولوژي Microbiology پژوهشي Research <pre dir="RTL" style="text-align:justify"> <span style="font-size:10pt"><span style="line-height:130%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">یکی از علت‌‌‌های اصلی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">پوسیدگی دندان و بیماری&shy;های پریودنتال </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">باکتری&shy;های موجود در حفره دهان می&shy;باشد. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">هدف این</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">تحقیق تعیین</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">فعالیت</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">ضد باکتری </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">چند عصاره گیاهی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">بر تعدادی از باکتری&shy;های حفره دهان </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">بود.</span></span></span><i></i></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:130%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">مواد و روش&shy;ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> این مطالعه آزمایشگاهی در دانشگاه </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">علوم پزشکی کردستان در سال 1400 انجام شد</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">.<b> </b>عصاره</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">الکلی</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">گیاهان میخک، رزماری، چای ترش، چای سبز و پونه کوهی با روش ماسراسیون</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">تهیه</span></span></span><i> </i><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">شد و</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> خاصیت ضد باکتری عصاره&shy;&shy;ها بر روی 5 سویه از باکتری&shy;های حفره دهان با دو روش چاهک‌گذاری و تعیین حداقل</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">غلظت مهاری </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">انجام شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> میانگین قطر هاله عدم رشد اندازه&shy;گیری و داده&shy;ها با استفاده از آنالیز واریانس یک&shy;طرفه و آزمون تعقیبی </span></span></span>Duncan <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">تجزیه و تحلیل شد.</span></span></span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:130%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">یافته‌ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> نتایج نشان داد </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">در میان عصاره&shy;ها بیشترین</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">اثر</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">ضد باکتری مربوط به عصاره میخک بر استرپتوکوک موتانس و سالیواریوس به ترتیب با میانگین و انحراف معیار 45/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%">&plusmn;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">03/20 و 20/0 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%">&plusmn;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> 06/19 میلی</span></span></span><i>‌</i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">متر بود. بر اساس مقایسه میانگین&shy;ها تأثیر عصاره&shy;ها و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">جنتامایسین</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> بر </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">قطر هاله عدم رشد دارای</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> تفاوت</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">معنی&shy;داری بود (05/0</span></span></span>P<i><</i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">) که این تفاوت در عصاره میخک بر استرپتوکوک موتانس مشاهده نشد (05/0<</span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">). حداقل</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">غلظت بازدارندگی</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">از</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">رشد، مربوط به</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">عصاره</span></span></span><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">میخک و</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> رزماری به ترتیب علیه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> استرپتوکوک موتانس و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">استافیلوکوک‌اورئوس </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">با غلظت 25/6 میلی‌گرم بر میلی‌لیتر بود.</span></span></span><i></i></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:130%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">نتیجه‌گیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> نتایج این تحقیق نشان داد که عصاره&shy;های این مطالعه به ویژه عصاره میخک می&shy;توانند به عنوان مواد ضد باکتر</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:">ی</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:130%"><span b="" nazanin="" style="font-family:"> مؤثر در درمان عفونت&shy;های باکتریایی حفره دهان مؤثر باشند.</span></span></span></span></span></span></span></span> <b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">واژه‌های</span></span></span><i> </i><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">کلیدی:</span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> ضد باکتری، حفره دهان</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">،</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> گیاهان دارویی، استافیلوکوک&shy;ها</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">استرپتوکوک&shy;ها </span></span></span> </pre> <div><div></div></div> <span style="font-size:11pt"><span style="line-height:150%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Yagut,Bold""></span></span></span></b></span></span></span></span></span></span> <pre> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><a name="_Hlk114425361"><b><span style="line-height:150%">Background and Objectives:</span></b></a> <span lang="EN" style="line-height:150%">One of the main causes of tooth decay and periodontal diseases in the oral cavity is bacteria. The aim of this research was to evaluate the antibacterial activity of several plant extracts on some of bacteria in the oral cavity.</span><span lang="AR-SA" dir="RTL" style="line-height:150%"></span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Materials and Methods: </span></b><span style="line-height:150%">This laboratory study </span><span lang="EN" style="line-height:150%">was conducted</span><span style="line-height:150%"> in Kurdistan University of Medical Sciences in 2021. Alcoholic extracts of <i>Eugenia caryophyllata,</i> <i>Rosmarinus officinalis</i>, <i>Hibiscus sabdariffa</i>, <i>Mentha</i> <i>pulegium, </i>and<i> Camellia sinensis</i>&nbsp;plants were prepared by a maceration method. Then the antibacterial properties of the extracts were determined on 5 strains of oral cavity bacteria by agar well diffusion and minimum inhibitory concentration methods. </span><span lang="EN" style="line-height:150%">The mean diameter of the growth inhibition zone</span> <span lang="EN" style="line-height:150%">was measured and the data was analyzed using one-way analysis of variance followed by Duncan&rsquo;s post hoc test.</span><span lang="AR-SA" dir="RTL" style="line-height:150%"></span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Results:</span></b><span style="line-height:150%"> The results showed that the most antibacterial effect was related to <i>Eugenia caryophyllata</i> extract and on <i>Streptococcus</i> <i>mutans and Salivarus</i> with mean and standard deviation of 20.03&plusmn;0.45 and 19.06&plusmn;0.20 mm, respectively.</span><span lang="EN" style="line-height:150%"> Based on the comparison of the means, the effect of extracts and gentamicin on </span><span style="line-height:150%">diameter of the zone of inhibition</span><span style="line-height:150%"> was significantly different (p<0.05), but this difference was not observed for </span><i><span style="line-height:150%">Eugenia caryophyllata</span></i><span style="line-height:150%"> extract</span><span lang="EN" style="line-height:150%"> on <i>Streptococcus mutans</i> (p>0.05). </span><span style="line-height:150%">The minimum inhibitory concentration of<i> Eugenia caryophyllata</i> and <i>rosemarinus</i> extracts against <i>Streptococcus mutans</i> and <i>Staphylococcus aureus</i> was 6.25 mg/ml. </span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Conclusion:</span></b><span style="line-height:150%"> The results of this research showed that the plant extracts, especially <i>Eugenia caryophyllata, </i></span><span lang="EN" style="line-height:150%">can be </span><span style="line-height:150%">effective as antibacterial substances in treating bacterial infections of the oral cavity.</span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Key words:</span></b><span style="line-height:150%"> Antibacterial, Oral cavity, Medicinal plants, Staphylococci, Streptococci</span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Funding:</span></b><span style="line-height:150%"> This study did not have any funds.</span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Conflict of interest: </span></b><span style="line-height:150%">None declared.</span><span lang="FA" dir="RTL" style="line-height:150%"></span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">Ethical approval: </span></b><span style="line-height:150%">The Ethics Committee of Kurdistan University of Medical Sciences approved the study (IR.MUK.REC.1397/5033).</span></span></span></span></span></span> <span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:140%">How to cite this article:</span></b><b> </b><span style="font-size:9.0pt"><span style="line-height:140%">Bahmani</span></span> <span style="font-size:9.0pt"><span style="line-height:140%">Nasrin.</span></span><span style="font-size:9.0pt"><span style="line-height:140%"> Antibacterial Effect of Alcoholic Extracts of the <i>Eugenia caryophyllata, Rosmarinus officinalis, Hibiscus sabdariffa, Camellia sinensis, </i>and <i>Mentha pulegium</i> Plants on Some of Bacteria in the Oral Cavity: A Laboratory Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:140%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:140%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:140%"> <i>Univ Med Sci</i> 2023; 22 (2): 147-60. [Farsi]</span></span> <b><span style="font-size:8.0pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span> </pre> ضد باکتری, حفره دهان, گیاهان دارویی, استافیلوکوک‌ها, استرپتوکوک‌ها Antibacterial, Oral cavity, Medicinal plants, Staphylococci, Streptococci 147 160 http://journal.rums.ac.ir/browse.php?a_code=A-10-6145-1&slc_lang=fa&sid=1 Nasrin Bahmani نسرین بهمنی nasrinbahmani3@gmail.com 6900319475328460053784 6900319475328460053784 Yes kurdistan university of medical sciences علوم پزشکی کردستان