fa تأثیر 8 هفته تمرین هوازی به همراه مصرف نانوکورکومین بر بیان ژن‌های Atrogin-1 وMurf-1 در عضله نعلی موش‌های صحرایی نر نژاد ویستار: یک گزارش کوتاه تربيت بدني تربيت بدني پژوهشي Research <br> <span style="font-size:10pt"><span style="line-height:125%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">پژوهش حاضر با هدف تعیین تأثیر هشت هفته مصرف نانوکورکومین و تمرین هوازی بر </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">بیان ژن&shy;های </span></span></span><i>Atrogin-1</i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> و </span></span></span><i>Murf-1</i><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> در عضله نعلی موش صحرایی نر نژاد ویستار انجام شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:125%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">مواد و روش&shy; ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> در این مطالعه تجربی، از </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">32 سر موش صحرایی استفاده شد. تمرینات ورزشی 5 جلسه در هفته انجام شد و مکمل&shy;دهی نیز (80 میلی&shy;گرم بر کیلوگرم) به موش‌ها</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> داده شد. از تکنیک </span></span></span>Real-time PCR<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> برای سنجش بیان ژن و جهت تجزیه و تحلیل داده&shy;ها از آنالیز واریانس دوطرفه استفاده شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:125%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:125%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">یافته ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> نتایج نشان داد که با انجام تمرین هوازی میانگین بیان ژن&shy;های </span></span></span>Murf-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> و </span></span></span>Atrogin-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> به طور معنی&shy;داری کاهش یافت (05/0</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%">></span></span>P<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">). هم&shy;چنین، مصرف مکمل نانوکورکومین هم منجر به کاهش میانگین بیان ژن&shy;های فوق شد (05/0</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%">></span></span>P<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:125%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">نتیجه&shy;گیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> مصرف مکمل نانوکورکومین و تمرینات هوازی هر کدام به تنهایی منجر به کاهش بیان ژن&shy;های </span></span></span>Atrogin-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> و </span></span></span>Murf-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> شدند ولی اثر متقابل آن‌ها بر بیان آتروژن&shy;ها معنی&shy;دار نبود.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:125%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">واژه&shy; های کلیدی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:"> تمرین هوازی، نانوکورکومین، </span></span></span>Murf-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">، </span></span></span>Atrogin-1<span lang="FA" style="font-size:12.0pt"><span style="line-height:125%"><span b="" nazanin="" style="font-family:">، موش صحرایی</span></span></span><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:125%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:135%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:135%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:160%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:160%"> The present study aimed to investigate the effects of eight weeks of taking nano-curcumin and performing aerobic exercise on the expression of Atrogin-1 and Murf-1 genes in the soleus muscle of male Wistar rats.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:160%"> This experimental study was conducted on 32 rats. Aerobic exercises were performed 5 times per week, and nano-curcumin was administered to rats at 80 mg per kg. The real-time PCR (Polymerase chain reaction) technique was employed to measure gene expression, and two-way analysis of variance (ANOVA) was used to analyze the data.&nbsp; </span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Results: </span></span></b><span style="font-size:9.0pt"><span style="line-height:160%">The results showed that aerobic exercise significantly decreased the expression levels of the MuRF-1 and Atrogin-1 genes (p<0.05). In addition, nano-curcumin supplementation without aerobic exercise also reduced the expression levels of the mentioned genes (p<0.05).</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Conclusion: </span></span></b><span style="font-size:9.0pt"><span style="line-height:160%">The results of this study indicated that nano-curcumin supplementation alone decreased the expression levels of the two mentioned genes and so did aerobic exercise alone. However, their interaction effects were not significant on the expression level of the Atrogin-1 gene.&nbsp; </span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Key words:</span></span></b><span lang="EN" style="font-size:9.0pt"><span style="line-height:160%"> Aerobic exercise, Nano-curcumin</span></span><span style="font-size:9.0pt"><span style="line-height:160%">, Murf-1, Atrogin-1, Rat</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:6.0pt"><span style="line-height:160%"></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:160%"> This study did not have any funds.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:160%"> None declared.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">Ethical approval:</span></span></b><span style="font-size:9.0pt"><span style="line-height:160%"> The Ethics Committee of Islamic Azad University of Parand approved the study (IR.IAU.PIAU.REC.1400.009).</span></span><span style="font-size:9.0pt"><span style="line-height:160%"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"><span style="line-height:160%"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:160%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:160%">How to cite this article: </span></span></b><span style="font-size:9.0pt"><span style="line-height:160%">Madani Fatemeh, Kazemzadeh Yaser. The Effect of 8 Weeks of Aerobic Exercise Along with Nano-Curcumin on the Expression of Atrogin-1 and Murf-1 Genes in Soleus Muscle of Male Wistar Rat: A Short Report</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:160%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:160%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:160%"> <i>Univ Med Sci</i> 2023; 22 (7): 769-76. [Farsi]</span></span></span></span></span></span></span><br> &nbsp; تمرین هوازی, نانوکورکومین, Murf-1, Atrogin-1, موش صحرایی Aerobic exercise, Nano-curcumin, Murf-1, Atrogin-1, Rat 769 776 http://journal.rums.ac.ir/browse.php?a_code=A-10-6235-1&slc_lang=fa&sid=1 Fatemeh Madani فاطمه مدنی ranamadani1000@yahoo.com 6900319475328460057107 0009-0008-9527-6515 No دانشکده تربیت بدنی دانشگاه آزاد اسلامی, واحد اسلامشهر, اسلامشهر, ایران YASER Kazem Zadeh یاسر کاظم زاده yaser.kazemzadeh@yahoo.com 6900319475328460057108 6900319475328460057108 Yes دانشکده تربیت بدنی دانشگاه آزاد اسلامی, واحد اسلامشهر, اسلامشهر, ایران