fa اثر عصاره آبی-الکلی مریم گلی (Salvia officinalis L.) بر کسب و بیان ترجیح مکان شرطی شده ناشی از مورفین در موش سوری: یک مطالعه تجربی فيزيولوژي Physiology پژوهشي Research <span style="font-size:10pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">اثرات پاداشی مورفین نقش مهمی در سوء مصرف آن دارد. هدف از مطالعه حاضر تعیین اثرات عصاره آبی-الکلی گیاه مریم گلی بر اثرات پاداشی مورفین در موش سوری ماده بود.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">مواد و روش</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">‌</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">این پژوهش </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">تجربی بر روی 136 سر موش سوری ماده انجام شد که به&shy;طورتصادفی به 17 گروه 8تایی تقسیم شدند. از روش ترجیح مکان شرطی شده (</span></span></span>Conditioned place preference; CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">) برای بررسی اثرات پاداشی داروها استفاده شد. در روزهای شرطی&shy;سازی، گروه&shy;های مختلف موش&shy;ها، مورفین 15-1</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">میلی&shy;گرم بر کیلوگرم و یا عصاره گیاه 150-50 میلی&shy;گرم بر کیلوگرم را دریافت کردند. گروه&shy;های کنترل نیز نرمال سالین دریافت نمودند. در آزمایش بیان، چهار گروه از موش&shy;ها پس از شرطی شدن با مورفین، یک ساعت قبل از آزمون </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">، عصاره گیاه یا نرمال سالین دریافت نمودند. در آزمایش کسب، به چهار گروه از حیوانات، یک ساعت قبل از دریافت مورفین در روزهای شرطی&shy;سازی، عصاره مریم گلی یا نرمال سالین تجویز شد. در روز بعد، آزمون </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> انجام شد. آنالیز داده&shy;ها توسط آنالیز واریانس یک&shy;طرفه و آزمون تعقیبی </span></span></span>Dunnett<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> انجام شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">یافته</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">‌</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> تجویز مورفین 15-1 میلی&shy;گرم بر کیلوگرم اثر معنی&shy;داری در القاء </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> داشت (001/0</span></span></span>P˂<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">). هم&shy;چنین، تجویز عصاره گیاه اثر معنی&shy;داری بر بیان </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> مورفین نداشت (05/0<</span></span></span>P<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">). بر عکس، تجویز عصاره مریم گلی باعث مهار کسب </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> ناشی از مورفین شد (001/0</span></span></span>P<<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:">نتیجه&shy;گیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> عصاره آبی-الکلی مریم گلی باعث مهار کسب </span></span></span>CPP<span lang="FA" style="font-size:12.0pt"><span style="line-height:117%"><span b="" nazanin="" style="font-family:"> ناشی ازمورفین می&shy;شود ولی بر بیان آن اثری ندارد. احتمالاً عصاره این گیاه را می&shy;توان برای پیش&shy;گیری از اثرات پاداشی مورفین به&shy;کار برد. </span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">واژه&shy;های کلیدی: </span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مریم گلی، مورفین، ترجیح مکان شرطی شده، موش سوری</span></span></span> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b></a> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">The rewarding effects of morphine play an important role in its abuse. This study aimed to determine the effects of </span></span><span style="font-size:9.0pt"><span style="line-height:150%">the hydro</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">-alcoholic extract of the sage plant on the rewarding effects of morphine in female mice.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">This experimental research was conducted on 136 female mice. The mice were randomly divided into 17 groups of eight. A conditioned place preference (CPP) procedure was used to investigate the rewarding effects of drugs. On conditioning days, different groups of mice received morphine (1-15 mg/kg) or plant extract (50-150 mg/kg). The control groups also received saline. In the expression test, four groups of mice, after being conditioned with morphine, received plant extract or saline one hour before the CPP test. In the acquisition experiment, </span></span><span style="font-size:9.0pt"><span style="line-height:150%">four groups of animals, </span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">one hour before administration of morphine on conditioning days, received sage extract or saline. On the next day, the CPP test was performed. Data were analyzed using one-way analysis of variance (ANOVA) and Dunnett&rsquo;s post-hoc test.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> A</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">dministration of morphine induced a significant CPP in mice (p<0.001). Additionally, administration of plant extract did not affect the expression of morphine CPP (p>0.05).</span></span> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">In contrast, the administration of sage extract inhibited the acquisition of morphine-induced CPP (p<0.001).</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">Hydro-alcoholic extract of sage inhibits the acquisition but not the expression of CPP caused by morphine. Probably, the extract of this plant can be used to prevent the rewarding effects of morphine.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">Key words:</span></span></b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"> Sage plant, Morphine, Classical conditioning, Mice</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding: </span></span></b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">This study did not have any funds.</span></span><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">None declared.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical approval:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">The Ethics Committee of University of Maragheh</span></span><span style="font-size:9.0pt"><span style="line-height:150%"> approved this study (IRUM-1400.003). </span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:8.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:150%">How to cite this article:</span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Barzegari Amir Abbas, Mortazavian Morteza. Effects of Hydro-Alcoholic Extract of Salvia Officinalis L. on the Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Mice: An Experimental Study. </span></span><i><span style="font-size:9.0pt"><span style="line-height:150%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Univ Med Sci</i> 2023; 22 (7): 725-42. [Farsi]</span></span> <b><span style="font-size:8.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br> &nbsp; Sage plant, Morphine, Classical conditioning, Mice 725 742 http://journal.rums.ac.ir/browse.php?a_code=A-10-6319-1&slc_lang=fa&sid=1 Amir Abbas Barzegari امیر عباس برزگری barzegaridoctora@gmail.com 6900319475328460055582 6900319475328460055582 Yes University of Maragheh دانشگاه مراغه Morteza Mortazavian مرتضی مرتضویان 00mortazavian00@gmail.com 6900319475328460055583 0009-0006-2219-504X No University of Maragheh دانشگاه مراغه