Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1402
9
1
gregorian
2023
12
1
22
9
online
1
fulltext
fa
بررسی بیان ژن ZBTB16 با تجزیه و تحلیل بیوانفورماتیک و روشهای ایمونوسیتوشیمی و ایمونوهیستوشیمی در سلولهای بنیادی شبه جنینی برگرفته شده از سلولهای بنیادی اسپرماتوگونیال بیضه موش: یک مطالعه آزمایشگاهی
Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study
زيست شناسي
زيست شناسي
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:105%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">سلولهای بنیادی اسپرماتوگونیال (</span></span></span>Spermatogonia stem cells; SSCs<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">) میتوانند سلولهای بنیادی شبه جنینی (</span></span></span>Eembryonic stem-like; ES-Like<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">) را تولید کنند که هر دو دارای پتانسیل سلولهای بنیادی پرتوان میباشند. ژن </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> (</span></span></span>Zinc Finger and BTB Domain Containing 16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">) عملکردهای مختلفی در مسیر سیگنالینگ، تنظیم تمایز و رشد سلولی دارد. هدف از پژوهش حاضر شناسایی مسیرهای سیگنالینگ مرتبط با ژن </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> با روشهای بیوانفورماتیک و بررسی میزان بیان این ژن در </span></span></span>SSCs<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> و </span></span></span>ES-Like<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> میباشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:105%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> در این مطالعه آزمایشگاهی، ابتدا 100 ژن دارای ارتباط و اتصال با </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> از پایگاه پروتئینی استخراج و توسط نرمافزارهای مرتبط آنالیز شدند. در ادامه، سلولهای </span></span></span>ES-Like<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> از </span></span></span>SSCs<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> بیضه موش استخراج و کشت داده شدند. سپس با انجام رنگآمیزی ایمونوسیتوشیمی و ایمونوهیستوشیمی بیان </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> در سلولهای </span></span></span>ES-Like<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> و </span></span></span>SSCs<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> بررسی شد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:105%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> نتایج نشان داد که بیان </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> در جمعیت تمایز نیافته </span></span></span>SSCs<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> و </span></span></span>ES-Like<span style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> به وضوح دیده میشود. همچنین، بیان شد که در صورت حذف </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> از شبکه ژنی میتوان در برخی از مسیرهای سیگنالینگ نامطلوب اختلال ایجاد کرد، زیرا </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> بهعنوان یک فاکتور رونویسی خاص در غنی کردن مسیرهای خاصی دخالت دارد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:105%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> یافتههای تجربی میتوانند از آنالیز رشد سلولهای اسپرماتوگونیال در </span></span></span>in vivo<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> و </span></span></span>in vitro<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> حمایت کنند و روشهای بیوانفورماتیک با شناسایی عملکردهای بیولوژیکی که </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> در تنظیم آنها دخیل میباشد، نشان دادند که برای ضعیف کردن برخی مسیرهای نامطلوب میتوان از </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> به عنوان ژن هدف استفاده نمود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:105%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""> سلولهای بنیادی اسپرماتوگونیال، سلولهای بنیادی شبه جنینی، مسیر سیگنالینگ، ارتباط پروتئین-پروتئین، </span></span></span>ZBTB16<span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:150%"> Spermatogonial stem cells (SSCs) can generate embryonic stem-like (ES-Like) cells, both of which possess the potential for pluripotent stem cells. The gene ZBTB16 (Zinc Finger and BTB Domain Containing 16) plays various roles in signaling pathways, cellular differentiation regulation, and growth. The aim of this research was to identify signaling pathways associated with the ZBTB16 gene using bioinformatics methods and investigate the expression levels of this gene in SSCs and ES-Like cells.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> In this experimental study, initially, 100 genes with connections to ZBTB16 were extracted from the protein database and analyzed using relevant software. Subsequently, ES-Like cells were extracted from mouse testicular SSCs and cultured. Then, the expression of ZBTB16 in ES-Like cells and SSCs was examined through immunocytochemistry and immunohistochemistry staining.</span></span><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Results: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">The results indicated that the expression of ZBTB16 was demonstrated in the undifferentiated population of SSCs and ES-Like cells. It was found that deleting ZBTB16 from the gene network could disrupt undesirable signaling pathways since ZBTB16 is involved in enriching specific pathways as a specific transcription factor.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The experimental findings can support the analysis of spermatogonial cell growth in both in vivo and in vitro. Bioinformatics methods, by identifying biological functions that ZBTB16 regulates, have shown that ZBTB16 can be used as a target gene to weaken certain undesirable pathways.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Key words:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Spermatogonial stem cells, Embryonic stem-like cells, Signaling pathways, Protein-protein interaction, ZBTB16</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> This study was funded by Amol University of Modern Technologies.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical approval:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The Ethics Committee of Amol University of Special Modern Technologies approved the study (IR.AUSMT.REC.1400.03).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><i><span lang="EN" style="font-size:2.0pt"><span style="line-height:150%"></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">How to cite this article:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Masoudi Mahla, Azizi Hossein, Gholami Dariush, Khaki Amir. Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:150%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Univ Med Sci</i> 2023; 22 (9): 947-62. [Farsi]</span></span> <b><span style="font-size:8.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
سلولهای بنیادی اسپرماتوگونیال, سلولهای بنیادی شبه جنینی, مسیر سیگنالینگ, ارتباط پروتئین-پروتئین, ZBTB16
Spermatogonial stem cells, Embryonic stem-like cells, Signaling pathways, Protein-protein interaction, ZBTB16
947
962
http://journal.rums.ac.ir/browse.php?a_code=A-10-6358-1&slc_lang=fa&sid=1
Mahla
Masoudi
مهلا
مسعودی
mahlamasoudii@gmail.com
6900319475328460055870
0009-0007-0191-9042
No
Amol University of Special Modern Technologies, Amol, Iran
دانشگاه فناوریهای نوین آمل
Hossein
Azizi
حسین
عزیزی
h.azizi@ausmt.ac.ir
6900319475328460055871
6900319475328460055871
Yes
Amol University of Special Modern Technologies, Amol, Iran
دانشگاه فناوریهای نوین آمل
Dariush
Gholami
داریوش
غلامی
d.gholami@ausmt.ac.ir
6900319475328460055872
6900319475328460055872
No
Amol University of Special Modern Technologies, Amol, Iran
دانشگاه فناوریهای نوین آمل
Amir
Khaki
امیر
خاکی
a.khaki@ausmt.ac.ir
6900319475328460055873
6900319475328460055873
No
Amol University of Special Modern Technologies, Amol, Iran
دانشگاه فناوریهای نوین آمل