Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1402
11
1
gregorian
2024
2
1
22
12
online
1
fulltext
fa
میزان استرس اکسیداتیو و التهاب در کلیه سمت راست پس از انسداد حالب چپ در موش صحرایی نر تحت درمان با تروگزروتین: یک مطالعه تجربی
Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin:
An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b></a><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">انسداد حالب</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یکی از عوامل آسیب کلیهها به شمار میآید. تروگزروتین یکی از آنتیاکسیدانهای قوی طبیعی است. هدف از انجام این مطالعه تعیین میزان استرس اکسیداتیو و التهاب در کلیه سمت راست پس از انسداد حالب چپ در موش صحرایی نر تحت درمان با تروگزروتین بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> در مطالعه تجربی حاضر، 18 سر موش صحرایی نر نژاد ویستار به صورت تصادفی به 3 گروه شش­تایی کنترل، انسداد حالب و انسداد حالب+تروگزروتین تقسیم شدند.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">پس از مسدود نمودن حالب چپ به مدت 3 روز، موشهای صحرایی، تروگزروتین (100 میلیگرم بر کیلوگرم) را روزانه، یکساعت پس از انسداد به مدت سه روز دریافت کردند. سپس</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">وزن کلیه راست و سطح نیتروژن اوره خون (</span></span></span>Blood urea nitrogen; BUN<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) اندازهگیری شد. میزان مالوندیآلدهید، سطوح فعالیت آنزیمهای گلوتاتیون پراکسیداز و سوپراکسید دیسموتاز و هم­چنین سطح بیان فاکتور نکروز توموری آلفا (</span></span></span>Tumor necrosis factor alpha; TNF-α<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) در بافت کلیه راست اندازهگیری شد. برای تجزیهوتحلیل دادهها از آنالیز واریانس یکطرفه و آزمون تعقیبی </span></span></span>Tukey<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> استفاده شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> انسداد حالب چپ باعث افزایش معنیدار میانگین وزن کلیه راست، غلظت مالوندیآلدهید و سطح بیان </span></span></span>TNF-α<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (002/0=</span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) و همچنین کاهش سطوح فعالیت آنزیمهای گلوتاتیون پراکسیداز (005/0=</span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) و سوپراکسید دیسموتاز (009/0=</span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) حیوانات گروه انسداد نسبت به کنترل شد. تروگزروتین توانست این شاخصها را در گروه انسداد حالب+تروگزروتین نسبت به گروه انسداد حالب تعدیل نماید (05/0></span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">).</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">میانگین </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">سطح </span></span></span>BUN<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، تفاوت معنیداری بین گروهها نشان نداد (05/0</span></span></span>P><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> به نظر می­رسد انسداد حالب چپ باعث القاء التهاب و استرس اکسیداتیو در کلیه سمت راست میشود و مصرف تروگزروتین باعث کاهش سطح استرس اکسیداتیو و التهاب میشود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> انسداد حالب، کلیه، استرس اکسیداتیو، التهاب، تروگزروتین، موش صحرایی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:115%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="line-height:115%">Background and Objectives:</span></b></a><span style="line-height:115%"> Ureteral obstruction is one of the causes of kidney damage. Troxerutin is one of the strong natural antioxidants. The purpose of this study was to investigate the levels of oxidative stress and inflammation in the right kidney tissue after the left ureteral obstruction in male rats.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:115%">Materials and Methods:</span></b><span style="line-height:115%"> In this experimental study, 18 male Wistar rats were randomly divided into 3 groups (n=6 in either group) including control, ureteral obstruction, and ureteral obstruction+troxerutin. After the left ureter occlusion for 3-day, rats received troxerutin (100 mg/kg) daily 1h after occlusion for three days. Then, the right kidney weight and the blood urea nitrogen (BUN) level were measured. The malondialdehyde concentration, the glutathione peroxidase, and superoxide dismutase enzyme activity levels, as well as the tumor necrosis factor-alpha (TNF-α) expression level, were measured in the right kidney tissue. One-way ANOVA and Tukey's post hoc test were used to analyze the data.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:115%">Results: </span></b><span style="line-height:115%">Left ureteral obstruction caused a significant increase in the right kidney weight, malondialdehyde concentration, and TNF-α expression level (P=0.002) and a decrease in the activity levels of glutathione peroxidase (P=0.005) and superoxide dismutase (P=0.009) enzymes of ureteral obstruction group versus the control. Troxerutin was able to ameliorate these indicators in the ureteral obstruction+troxerutin group compared to the ureteral obstruction group (P<0.05). Blood urea nitrogen level mean did not show significant differenceacross the groups (P>0.05).</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:115%">Conclusion: </span></b><span style="line-height:115%">Left ureter obstruction induces inflammation and oxidative stress in the right kidney, and troxerutin consumption reduces inflammation and oxidative stress levels.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:115%">Key words: </span></b><span style="line-height:115%">Ureteral obstruction, Kidney, Oxidative stress, Inflammation, Troxerutin, Rat</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span style="font-size:6.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:150%">Funding:</span></b><span style="line-height:150%"> This study was funded by Rafsanjan University of Medical Sciences.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:150%">Conflict of interest:</span></b><span style="line-height:150%"> None.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:150%">Ethical approval:</span></b><span style="line-height:150%"> The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1397.079).</span><span lang="FA" dir="RTL" style="line-height:150%"></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:4.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:140%">How to cite this article:</span></b><span style="font-size:9.0pt"><span style="line-height:140%"> Kaeidi A, Hassanshahi J. Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin: An Experimental Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:140%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:140%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:140%"> <i>Univ Med Sci</i> 2024; 22(12): 1335-46. [Farsi]</span></span> <span style="font-size:9.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
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<span style="line-height:115%"><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span>
انسداد حالب, کلیه, استرس اکسیداتیو, التهاب, تروگزروتین, موش صحرایی
Ureteral obstruction, Kidney, Oxidative stress, Inflammation, Troxerutin, Rat
1335
1346
http://journal.rums.ac.ir/browse.php?a_code=A-10-1742-5&slc_lang=fa&sid=1
Ayat
Kaeidi
آیت
کائیدی
a.kayedi@gmail.com
6900319475328460056613
6900319475328460056613
No
Rafsanjan University of Medical Sciences
دانشگاه علوم پزشکی رفسنجان، رفسنجان
Jalal
Hassanshahi
جلال
حسن شاهی
hasanshahij@gmail.com
6900319475328460056614
6900319475328460056614
Yes
Rafsanjan University of Medical Sciences
دانشگاه علوم پزشکی رفسنجان، رفسنجان