Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1403
8
1
gregorian
2024
11
1
23
7
online
1
fulltext
fa
اثر محافظتی عصاره آبی-الکلی میوه گیاه سپستان در کاهش سمیت ناشی از پراکسید هیدروژن در سلولهای اندوتلیال آئورت گاو (BAE-1):
یک مطالعه آزمایشگاهی
Protective Effect of Hydroalcoholic Extract of Cordia Myxa Fruit in Decreasing H2O2-Induced Toxicity in Bovine Aortic Endothelial Cells (BAE-1): A Laboratory Study
فارماكولوژي
Pharmacology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> با توجه به اثرات منفی استرس اکسیداتیو در پاتوفیزیولوژی بیماریهای قلبی و عروقی، استفاده از آنتیاکسیدانها مانند گیاهان دارویی میتواند به کاهش عوارض کمک کند. این مطالعه با هدف تعیین اثر محافظتی گیاه سپستان در برابر استرس اکسیداتیو ناشی از پراکسید هیدروژن (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">H<sub>2</sub>O<sub>2</sub></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) در سلولهای اندوتلیوم آئورت جدا شده از گاو (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">Bovine Aortic Endothelial; BAE-1</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) انجام شد.</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> در این مطالعه آزمایشگاهی</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">ابتدا عصاره آبی-الکلی از میوه گیاه سپستان تهیه شد. سلول</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%"></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">BAE-1</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> به مدت 24 ساعت در مجاورت غلظتهای مختلف عصاره (200-25 میکروگرم در میلیلیتر) قرار گرفتند، سپس </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">H2O2</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> (200 میکرومولار) اضافه شد و پس از نیم­ساعت تستهای مختلف شامل زندهمانی سلولها، میزان آپوپتوز سلولی به روش فلوسایتومتری، بررسی پراکسیداسیون لیپیدی با سنجش مالوندیآلدهید و بررسی تولید رادیکالهای آزاد با پروب دی­کلرودی­فلئورسین­دی استات (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">Dichlorodifluorescein diacetate; DCFDA</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) انجام گردید. دادهها با استفاده از آنالیز واریانس یکطرفه و آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">Tukey</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شدند.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> غلظتهای مختلف عصاره میوه سپستان به­صورت وابسته به دوز میزان تولید گونه­های فعال اکسیژن و مالوندیآلدهید ناشی از سمیت </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">H<sub>2</sub>O<sub>2</sub></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> را به صورت معنیدار نسبت به گروه </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">H<sub>2</sub>O<sub>2</sub></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> کاهش داد (05/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">). هم­چنین، پیش­تیمار سلول­ها با عصاره میوه سپستان به صورت وابسته به دوز باعث کاهش معنیدار میزان آپوپتوز نسبت به گروه پراکسید هیدروژن شد (010/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">بر اساس یافتههای پژوهش حاضر، به نظر می­رسد استفاده از عصاره آبی-الکلی میوه سپستان می­تواند سبب کاهش آسیب اکسیداتیو و سمیت سلولی ناشی از پراکسید هیدروژن در سلولهای </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">BAE-1</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> شود.</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%"></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">واژه­های کلیدی:</span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""> سپستان، پراکسید هیدروژن، استرس اکسیداتیو، سلول اندوتلیال</span></span></span>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Considering the negative impact of oxidative stress on the pathophysiology of cardiovascular diseases, the use of antioxidants such as medicinal plants can help reduce complications. This study was conducted with the aim of determining the protective effect of <i>Cordia<b> </b>myxa</i> (<i>C. myxa</i>) fruit<b> </b>against oxidative stress caused by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in bovine aortic endothelium cells (BAE-1).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> In this laboratory study, hydro-alcoholic extract was prepared from the fruit of <i>C. myxa</i><b> </b>plant. The cells were exposed to different concentrations of extract (25-200 µg/ml) for 24h, then H<sub>2</sub>O<sub>2</sub> (200 µM) was added and after 30 min, various tests were carried out including cell viability, cell apoptosis (Flow cytometry technique), lipid peroxidation (Malondialdehyde [MDA]) assay, and evaluation of reactive oxygen species (ROS, by </span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">dichlorodifluorescein diacetate probe). </span></span><span style="font-size:9.0pt"><span style="line-height:150%">Data analysis was done using one-way analysis of variance followed by Tukey’s post hoc test. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Different concentrations of the fruit extract significantly decreased ROS production and MDA level as compared to the H<sub>2</sub>O<sub>2</sub> group in a dose-dependent manner (p<0.05). Also, pretreatment with <i>C. myxa</i> fruit extract significantly reduced the rate of apoptosis compared to the H2O2 group dose dependently (p<0.010).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Based on the findings of the present study, it appears that the use of the hydro-alcoholic extract of <i>C. myxa</i> fruit can reduce the oxidative damage and cytotoxicity induced by H2O2 in BAE-1 cells.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Keywords:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Cordia myxa, Hydrogen peroxide, Oxidative stress, Endothelial cell</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span style="font-size:6.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> This study was funded by Mashhad University of Medical Sciences.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical considerations:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The Ethics Committee of Mashhad University of Medical Sciences approved the study (IR.MUMS.MEDICAL.REC.1398.239).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Authors’ contributions:</span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Conceptualization:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Azar Hoseini </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Methodology:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Azar Hoseini, Sahar Fanoudi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Data collection:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Sahar Fanoudi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Formal analysis:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Sahar Fanoudi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Supervision:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Azar Hoseini</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Project administration:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Azar Hoseini</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing – original draft:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Sahar Fanoudi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing – review & editing:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Azar Hoseini, Sahar Fanoudi</span></span><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
سپستان, پراکسید هیدروژن, استرس اکسیداتیو, سلول اندوتلیال
Cordia myxa, Hydrogen peroxide, Oxidative stress, Endothelial cell
605
621
http://journal.rums.ac.ir/browse.php?a_code=A-10-6779-1&slc_lang=fa&sid=1
Sahar
Fanoudi
سحر
فنودی
fanoudis921@gmail.com
6900319475328460058712
6900319475328460058712
No
neyshabur university of medical sciences
دانشگاه علوم پزشکی نیشابور
Azar
Hoseini
آذر
حسینی
hoseiniaz@mums.ac.ir
6900319475328460058713
6900319475328460058713
Yes
Mashhad university of medical sciences
دانشگاه علوم پزشکی مشهد