Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1403
8
1
gregorian
2024
11
1
23
7
online
1
fulltext
fa
اثر محافظتی عصاره هیدروالکلی کاکوتی کوهی (Ziziphora clinopodioides) بر سمیت
کبدی-کلیوی نانوذرات نقره در موشهای صحرایی نر: یک مطالعه تجربی
Protective Effect of Hydroalcoholic Extract of Ziziphora clinopodioides on Hepato-Renal Toxicity of Silver Nanoparticles in Male Rats: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b></a><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> مطالعات سمشناسی روی نانوذرات نقره (</span></span></span>Ag-NPs<span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) به دلیل کاربردهای زیستپزشکی گسترده اهمیت زیادی دارد. به دلیل خاصیت آنتیاکسیدانی بالای کاکوتی کوهی، هدف از مطالعه حاضر تعیین تأثیر محافظتی این گیاه در برابر سمیت ناشی از </span></span></span>Ag-NPs<span style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> بود. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">مواد و روش</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">­</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">ها:</span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">در این مطالعه تجربی، تعداد 30 سر موش صحرایی نر در محدوده وزنی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">20</span></span></span>±<span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">200 گرم به 5 گروه 6 تایی تقسیم شدند که شامل گروه کنترل، گروه دریافتکننده </span></span></span>Ag-NPs<span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> با دوز </span></span></span>ppm<span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">200، گروههای دریافت­کننده حیوانات عصاره هیدروالکلی کاکوتی کوهی با دوزهای 20، 60 و 180 میلیگرم بر کیلوگرم همراه با (</span></span></span> ppm<span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">200)</span></span></span>Ag-NPs <span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> بودند. تجویز ترکیبات به صورت خوراکی و یکبار در روز بهمدت 30 روز بود. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">در پایان </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">مطالعه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">، نمونههای خون جمعآوری و فعالیت</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> سرمی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">آلکالین فسفاتاز، آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز، لاکتات دهیدروژناز و غلظت سرمی اوره و کراتینین اندازهگیری شدند. داده­ها با استفاده از آنالیز واریانس </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یک­طرفه </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">و آزمون تعقیبی </span></span></span>Tukey<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> تجزیه­ و تحلیل شدند.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> نتایج نشان داد که تجویز </span></span></span>Ag-NPs <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">فعالیت</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> سرمی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">آلکالین فسفاتاز، آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز، لاکتات دهیدروژناز و غلظت سرمی اوره و کراتینین را </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">به طور معنی­داری </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">در مقایسه با گروه کنترل افزایش داد (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">05/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">></span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">). این تغییرات طی درمان با عصاره کاکوتی کوهی در دوزهای مختلف نسبت به گروه دریافت­کننده </span></span></span>Ag-NPs<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> بهبود یافت (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">05/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">></span></span></span>P<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">). </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">نتیجهگیری</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">بر اساس نتایج مطالعه حاضر </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">کاکوتی کوهی ممکن است در پیش­گیری از </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">سمیت </span></span></span>Ag-NPs<span style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> مؤثر باشد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">واژههای کلیدی: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">نانوذرات نقره، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">کاکوتی کوهی،</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">سمیت کبدی-کلیوی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">،</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> موش صحرایی</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="line-height:112%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">ارجاع:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin""> خزائی م، پویانمهر م، ملکی ع، علیمحمدی ص، حقنظری ل،</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">اثر محافظتی عصاره هیدروالکلی کاکوتی کوهی (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:112%">Ziziphora clinopodioides</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">) بر سمیت کبدی-کلیوی نانوذرات نقره در موش</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"Cambria",serif"></span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">های صحرایی نر: یک مطالعه تجربی</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:112%"><span style="font-family:"B Nazanin"">. <i>مجله دانشگاه علوم پزشکی رفسنجان</i>، سال 1403، دوره 23 شماره 7، صفحات: 588-578.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:150%"> Toxicological studies on silver nanoparticles (Ag-NPs) have great importance due to their extensive</span></span> <span style="font-size:9.0pt"><span style="line-height:150%">biomedical applications. Because of high antioxidant property of <i>Ziziphora clinopodioides</i>, the aim of this study was to investigate the protective effect of this plant against Ag-NPs-induced toxicity. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">In this experimental study, 30 male rats weighing 200±20 g were divided into 5 groups of 6 which included the control group, the group receiving Ag-NPs with a dose of 200 ppm, the groups receiving the hydroalcoholic extract of Ziziphora clinopodioides with doses of 20, 60, and 180 mg/kg along with Ag-NPs (200 ppm). The compounds were administered orally once a day for 30 days. At the end of the treatment period, blood samples were collected and serum activity of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and serum concentration of urea and creatinine were evaluated. Data were analyzed by one-way analysis of variance and Tukey’s post hoc test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The results indicated that administration of Ag-NPs significantly increased the serum activity of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and serum concentration of urea and creatinine compared to the control group (p<0.05). These changes were improved during treatment with the extract of <i>Ziziphora clinopodioides</i> in different doses compared to the group receiving Ag-NPs (p<0.05).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Based on the results of the present study, the <i>Ziziphora clinopodioides</i> may be effective in preventing the toxicity of Ag-NPs.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Keywords:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Silver nanoparticle, <i>Ziziphora clinopodioides</i>, Hepato-Renal toxicity, Rat</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:2.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> This study was funded by Razi University, Kermanshah, Iran.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical considerations:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The Ethics Committee of Razi University approved the study (Code of ethics: 396-2-039).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Authors’ contributions: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Conceptualization: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi, Mehrdad Pooyanmehr</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Methodology: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi, Mehrdad Pooyanmehr</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Data collection: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Mehrdad Pooyanmehr, Milad Khazaie,<b> </b>Ali Maleki, Lida Haghnazari</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Formal analysis: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi, Mehrdad Pooyanmehr, Milad Khazaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Supervision: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi, Mehrdad Pooyanmehr</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Project administration: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi, Mehrdad Pooyanmehr</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing - original draft: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi</span></span></span></span></span></span></span><br>
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<div><span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:150%">Citation: </span></b><span style="font-size:9.0pt"><span style="line-height:150%">Khazaie M, Pooyanmehr M, Maleki A, Alimohammadi S, Haghnazari L.</span></span><b> </b><span style="font-size:9.0pt"><span style="line-height:150%">Protective Effect of Hydroalcoholic Extract of Ziziphora clinopodioides on Hepato-Renal Toxicity of Silver Nanoparticles in Male Rats: An Experimental Study</span></span><span style="font-size:9.0pt"><span style="line-height:150%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:150%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Univ Med Sci</i> 2024; 23 (7): 578-88. [Farsi]</span></span></span></span></span></div>
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</v:textbox> <w:wrap anchorx="margin"> </w:wrap></v:roundrect><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing - review & editing: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Samad Alimohammadi</span></span><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span style="font-size:9.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
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<div></div>
نانوذرات نقره, کاکوتی کوهی, سمیت کبدی-کلیوی, موش صحرایی
Silver nanoparticle, Ziziphora clinopodioides, Hepato-Renal toxicity, Rat
578
588
http://journal.rums.ac.ir/browse.php?a_code=A-10-4606-4&slc_lang=fa&sid=1
Milad
Khazaie
میلاد
خزائی
Khazaeimilad72@gmail.com
6900319475328460058714
0009-0009-3074-7096
No
Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.
Mehrdad
Pooyanmehr
مهرداد
پویان مهر
M.pooyanmehr@razi.ac.ir
6900319475328460058715
6900319475328460058715
No
Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.
Ali
Maleki
علی
ملکی
a.maleki@kums.ac.ir
6900319475328460058716
6900319475328460058716
No
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
مرکز تحقیقات بیولوژی پزشکی، پژوهشکده فناوری سلامت، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه ، ایران
Samad
Alimohammadi
صمد
علی محمدی
S.alimohammadi@razi.ac.ir
6900319475328460058717
6900319475328460058717
Yes
Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.
Lida
Haghnazari
لیدا
حق نظری
Lida_Haghnazari@yahoo.com
6900319475328460058718
6900319475328460058718
No
Department of Clinical Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران