Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1405
3
1
gregorian
2026
6
1
25
1
online
1
fulltext
fa
بررسی فراوانی ژنهای nheA و cytK در باسیلوس سرئوسهای جدا شده از ماهی قزلآلای تازه عرضه شده در مراکز پخش ماهی تهران: یک مطالعه آزمایشگاهی
Study of nheA and cytK Genes Frequency in Bacillus Cereus Isolated from Fresh Rainbow Trout Supplied in Fish Distribution Centers in Tehran: A Laboratory Study
ميكروبيولوژي
Microbiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:110%"><span style="font-family:"B Nazanin"">چکیده</span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> غذاهای دریایی ارزش غذایی بسیار بالایی دارند و میتوانند بر سلامتی جامعه مؤثر باشند. <i>باسیلوس سرئوس</i> یکی از مهمترین عوامل بیماریزای مسمومیتهای ناشی از غذاهای دریایی است. هدف از پژوهش حاضر تعیین فراوانی ژنهای </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">nheA</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">cytK</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> در <i>باسیلوس سرئوسهای</i> جدا شده از ماهی قزلآلا بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مواد و روش</span></span></span></b><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ها</span></span></span></b><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">:</span></span></span></b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> در این مطالعه آزمایشگاهی، 30 نمونه ماهی قزلآلای تازه عرضه شده در مراکز پخش ماهی تهران</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> در سال 1403، </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">بهصورت تصادفی جمعآوری و به آزمایشگاه انتقال داده شد.</span></span></span> <span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نمونهها</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> در </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">محیط کشت </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">BHI</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Brain heart infusion</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) آگار کشت</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> داده شدند.</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> سپس کشت تککلنیها در محیط کشت اختصاصی </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">MYP</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Mannitol egg Yolk Polymyxin</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) آگار انجام شد و رنگآمیزی گرم، تست کاتالاز و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">PCR</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> برای ژن </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">16S rRNA</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> به­منظور جداسازی و </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">شناسایی</span></span></span> <span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">باکتری <i>باسیلوس سرئوس</i></span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> انجام گردید. توالییابی ژن </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">16S rRNA</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">، </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">BLAST</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Basic Local Alignment Search Tool</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) در سایت </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">NCBI</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">National Center for Biotechnology Information</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) آنالیز فیلوژنتیکی</span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> این ژن </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">توسط نرمافزار </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">MEGA 6.0</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> انجام شد</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> و </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ژنهای بیماریزای (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">nheA</span></span><b> </b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">و</span></span></span><b> </b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">cytK</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) در باکتری های جداسازی شده، توسط </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">PCR</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> شناسایی گردید.</span></span></span> <span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">دادهها با آزمون آماری </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">t</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> مستقل تجزیه و تحلیل شدند.</span></span></span><b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b> <span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">از 30 نمونه بررسی شده، 2 نمونه (6/66 درصد)</span></span></span> <i><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">باسیلوس</span></span></span></i><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> <i>سرئوس</i> جداسازی و شناسایی شد و </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">100 درصد آنها حامل </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ژنهای بیماریزای </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">nheA</span></span><b> </b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">و</span></span></span><b> </b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">cytK</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> بودند</span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">.</span></span></span><b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مطالعه حاضر نشان داد جلوگیری از آلودگی غذاهای دریایی به باکتری </span></span></span><i><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">باسیلوس</span></span></span></i><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> <i>سرئوس</i></span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> ضروری میباشد، زیرا تمامی </span></span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">سویههای جداسازی شده </span></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">دارای ژنهای حدت بودند. مطالعات بیشتر در این زمینه پیشنهاد میگردد.</span></span></span><b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> <i>باسیلوس</i> <i>سرئوس</i>، ژنهای </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">nheA</span></span><b> </b><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">و</span></span></span><b> </b><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">cytK</span></span><span lang="AR-SA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">، ماهی قزلآلا</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
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" style="position:absolute; left:0; text-align:left; margin-left:21px; margin-top:2px; width:467.05pt; height:49.55pt; z-index:-251648512; v-text-anchor:top"> <w:wrap anchorx="margin"> </w:wrap></v:roundrect><b><span lang="FA" style="font-size:3.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">ارجاع:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">سیاسی ا، دانشور ا، تکبیری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">ل. بررسی فراوانی ژنهای </span></span></span>nheA<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> و </span></span></span>cytK<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> در باسیلوس سرئوسهای جدا شده از ماهی قزل</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">آلای تازه عرضه شده در مراکز پخش ماهی تهران: یک مطالعه آزمایشگاهی</span></span></span><span lang="FA" style="line-height:120%"><span style="font-family:"B Nazanin"">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> سال 1405، دوره 25، شماره 1، صفحات: 62-51.</span></span></span><span lang="FA" style="font-size:1.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:140%">Background and Objectives:</span></span></b></a> <span style="font-size:9.0pt"><span style="line-height:140%">Seafood has a very high nutritional value and can have effect on social health. <i>Bacillus cereus </i>is one of the most important pathogens of seafood poisoning. This study was aimed to study <i>nheA</i></span></span> <span style="font-size:9.0pt"><span style="line-height:140%">and <i>cytK</i></span></span> <span style="font-size:9.0pt"><span style="line-height:140%">genes frequency in <i>Bacillus cereus</i> isolated from fresh rainbow trout.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> In this in vitro study, thirty samples of fresh rainbow trout, supplied in fish distribution centers in Tehran, were randomly collected in 2024, and transferred to the laboratory. The samples were cultured in BHI (Brain heart infusion) agar medium. Then colonies were cultured in MYP <i>(</i>Mannitol egg Yolk Polymyxin) agar-specific medium, and Gram staining, catalase testing, and PCR targeting the 16S rRNA gene were performed to isolate and identify <i>Bacillus cereus</i>. Sequencing of the 16S rRNA gene, BLAST (Basic Local Alignment Search Tool) analysis using the National Center for Biotechnology Information (NCBI) database, and phylogenetic analysis using MEGA 6.0 were performed, and virulence genes (<i>nheA</i> and <i>cytK</i>) were identified by PCR in the isolated <i>Bacillus cereus</i> bacteria. Data was analyzed by t-test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> From 30 studied samples, 2 samples (6.66%) of <i>Bacillus cereus</i> were isolated and identified, and 100% of them carried nheA and cytK pathogen genes.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> The present study indicated that preventing contamination of seafood with <i>Bacillus cereus</i> is essential, as all isolated strains carried virulence genes. Further research on this topic is recommended.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Keywords:</span></span></b><span style="font-size:9.0pt"><span style="line-height:140%"> <i>Bacillus cereus</i>, <i>nheA</i></span></span> <span style="font-size:9.0pt"><span style="line-height:140%">and</span></span><b> </b><i><span style="font-size:9.0pt"><span style="line-height:140%">cytK</span></span></i> <span style="font-size:9.0pt"><span style="line-height:140%">genes, Rainbow trout </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Funding: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">This study did not have any funds.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Ethical approval: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">The Ethics Committee of NT.C., Islamic Azad University, Tehran Branch, approved the study (IR.IAU.TNB.REC.1403.135).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Conflict of interest: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">None declared. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">Authors’ contributions:</span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Conceptualization: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elham Siasi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Methodology: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elahe Daneshvar </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Data collection: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elahe Daneshvar </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Formal analysis: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elham Siasi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Supervision: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Laya Takbiri</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Project administration: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elham Siasi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Writing – original draft: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elham Siasi</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:140%">- Writing – review & editing: </span></span></b><span style="font-size:9.0pt"><span style="line-height:140%">Elham Siasi, Laya Takbiri</span></span><span style="font-size:9.0pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
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<div><span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:8.0pt"><span style="line-height:150%">Citation: </span></span></b> <span style="font-size:8.0pt"><span style="line-height:150%">Siasi E, Daneshvar E, Takbiri L. </span></span><span lang="TR" style="font-size:8.0pt"><span style="line-height:150%">Study of nheA and cytK Genes Frequency in Bacillus Cereus Isolated from Fresh Rainbow Trout Supplied in Fish Distribution Centers in Tehran: A Laboratory Study</span></span><span style="font-size:8.0pt"><span style="line-height:150%">. <i>J Rafsanjan</i> <i>Univ Med Sci</i> 2026 Apr; 25 (1): 51-62.</span></span> <span style="font-size:8.0pt"><span style="line-height:150%">doi: 1066224/jrums.25.1.51 [Farsi]</span></span></span></span></span></div>
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باسیلوس سرئوس, ژنهای nheA و cytK, ماهی قزلآلا
Bacillus cereus, nheA and cytK genes, Rainbow trout
51
62
http://journal.rums.ac.ir/browse.php?a_code=A-10-7200-1&slc_lang=fa&sid=1
Elham
Siasi
الهام
سیاسی
emi_biotech2006@yahoo.ca
6900319475328460062790
6900319475328460062790
Yes
Department of Microbiology, School of science, NT.C., Islamic Azad University, Tehran, Iran.
گروه میکروبیولوژی، دانشکده علوم زیستی، واحد تهران شمال، دانشگاه آزاد اسلامی ، تهران، ایران.
Elahe
Daneshvar
الهه
دانشور
Daneshvarelahe2@gmail.com
6900319475328460062791
0009-0002-0791-617X
No
Department of Microbiology, School of science, NT.C., Islamic Azad University, Tehran, Iran.
گروه میکروبیولوژی، دانشکده علوم زیستی، واحد تهران شمال، دانشگاه آزاد اسلامی ، تهران، ایران.
Laya
Takbiri
لعیا
تکبیری
Laya6076@yahoo.com
6900319475328460062792
6900319475328460062792
No
Department of Microbiology, School of science, NT.C., Islamic Azad University, Tehran, Iran.
گروه میکروبیولوژی، دانشکده علوم زیستی، واحد تهران شمال، دانشگاه آزاد اسلامی ، تهران، ایران.