Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
91
100
OTHER
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
Effect of GABAB Agonist and Antagonist Microinjection into Cuneiformis Nucleus (CNF) on Morphine Induced Antinociception by Formalin Test in Rat
101
108
FA
M.
Rezvanipour
V.
Sheibani
M.
Bakhshesh
Effect of GABAB Agonist and Antagonist Microinjection into Cuneiformis Nucleus (CNF) on Morphine Induced Antinociception by Formalin Test in Rat M. RezvanipourPhD , V. Sheibani PhD , M. Bakhshesh MSc Received: 21/05/06 Sent for Revision: 25/06/06 Received Revised Manuscript: 11/12/06 Accepted: 27/01/07 Background and Objective: There are several evidences that show cuneiformis nucleus is involved in nociception. In the present study the effect of intra cuneiformis microinjection of GABAB agonist (baclofen) and antagonist (CGP35348) on morphine induced antinociception in rat were investigated. Materials and Methods: In this expremental study, through canulation of cuneifoprmis nucleus in rat the effect of intra cuneiformis (CNF) microinjection of GABAB receptor agonist (baclofen) and antagonist (CGP35348) on morphine – iduced antinociception were investigated by formalin test. Results: Microinjection of morphine (10μg /0.5 μl/saline) or different doses of baclofen (0.25,0.5,1μg per rat) had antinociception in the both first and second phases of formalin test. The response induced by morphine or baclofen in both phases were reduced by CGP35348. The responses induced by combination of baclofen (1μg per rat) and intraperitoneal (ip) injection of naloxan were reduced in both phases of formalin test. Microinjection of CGP35348 alone has produced antinociception in first phase of the formalin test. Morphine with different doses of baclofen did not increase the antinociception effect whereas microinjection of CGP35348 administration significantly increased the antinociception in acute phase. Conclusion: It may be concluded that CNF GABAB receptor induced antinociception via opioid receptor in the formalin test. Key words: Cuneiformis, CGP35348, Baclofen, GABAB, Morphine
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
109
114
OTHER
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
115
122
OTHER
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
In vitro Comparison of the Apical Microleakage of Lateral Compaction with the Warm Vertical of 4% Tapered Gutta-Percha Obturation in Root Canal Prepared by FlexMaster Rotary System
123
128
FA
K.
Nazari Moghadam
K.
Honardar
H.
Labaf
In vitro Comparison of the Apical Microleakage of Lateral Compaction with the Warm Vertical of 4% Tapered Gutta-Percha Obturation in Root Canal Prepared by FlexMaster Rotary System K. Nazari Moghadam DDs , K. Honardar DDs , H. Labaf DDs2 Received: 19/06/06 Sent for Revision: 28/06/06 Received Revised Manuscript: 29/04/07 Accepted: 09/05/07 Background and Objective: The success rate of root canal treatment depends on the proper execution of the final phase. Recently, various materials and techniques have been presented on the market for root canal obturation. The purpose of this study was to compare in vitro lateral condensation technique of the microleakage seal of with warm vertically-obturated single 4% tapered gutta-percha cone in root canals prepared by rotary system FlexMaster in crown-down manner. Materials and Methods: In this laboratory study the mesial root canals of 36 freshly extracted human mandibular molar teeth randomly were divided in to two groups of 15 teeth and two groups were designate as a negative and positive control respectively. After cleaning and shaping with FlexMaster rotary system, the teeth were obturated as follows: in group one, a lateral compaction technique with 2% tapered gutta-percha and in group two, obturated warm vertically (ENDOTWIN) with a single 4% tapered gutta-percha cone and AH plus endodontic sealer were applied. Nevertheless, the coronal portion and the root surface of each tooth was covered with two layers of nail varnish and a layer of sticky wax. The specimens were then immersed in blue Indian ink dye for two days, prior to sectioning by disc. Dye penetration was evaluated, and the data were analyzed with T-student test. Results: There was no significant difference between the two groups (p=0.83). The mean of dye penetration in lateral compaction group was 0/65 mm and for warm vertically compaction group was 0/61mm. Conclusion: This study showed that canals prepared with Flex Master rotary systems can be obturated with 2% taper gutta-percha plus AHPLUS root canal sealer. Thus lateral compaction could possibly be a suitable technique for root canal obturation and other requirements. Key words: Root canal preparation, Flexmaster rotary system, 4% Tapered gutta-percha, Cold Lateral Compaction , Endotwin, Plugger, Apical microleakage
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
129
134
OTHER
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
Comparison of Embryo Quality Cultured in Ham's F-10+%10 Maternal Serum and GIIII
135
142
FA
S.G.A.
Jorsaraie
M.
Zeinalzadeh
T.
Nazari
Y.R.
YousofniaPasha
A.A.
Beiki
A.
Bazram
Comparison of Embryo Quality Cultured in Ham's F-10+%10 Maternal Serum and GIIII S.G.A. Jorsaraie PhD , M. Zeinalzadeh MD , T. Nazari MD2, Y.R. YousofniaPasha MD A.A. Beiki MPh , A. Bazram GP Received: 10/01/07 Sent for Revision: 17/04/07 Received Revised Manuscript: 07/08/07 Accepted: 26/08/07 Background and Objectives: Appropriate culture media and environment for embryonic cell growth considered to be major factors for generation of an embryo in vitro. In this study two embryonic growth condition of GIIII and Ham’s F-10 + Maternal Serum (MS) %10 were compared. Materials and Methods: This investigation was prospectively performed on 100 infertile couples that were treated by IVF-ICSI. The participants were divided into two equal groups. Ovules obtained from first group were treated with Ham’s F10 + Maternal Serum %10 culture media and the second group with GIIII. The variables affecting ovule and embryonic growth were measured, and collected data were analyzed by SPSS. Reasults: The mean of age for tested ladies was 28.01 and the mean for duration of infertility was 6.87 years. The number of pronucleous with moderate quality were 2.14 in-group I and 3.22 in-group II (P=0.023). The number of embryo with three cells were 1.26 and 0.54 and for grade B embryonic cell were 1.18 and 1.78 and grade C embryonic cell were 1.08 and 0.56 in group I and II respectively (p=0.29). The mean of grade B blastomer in GIIII media was more than Ham’s F10 + Maternal Serum 10%. Compairing the means the mean of grade B blastomers in GIIII media was shown to be more than Ham’s F10 + Maternal Serum 10%. Conclusion: All of the findings showed that GIIII cultural condition is more effective than Ham’s F10 and the maternal serum as a supplement has no considerable impact. Key words: IVF, Culture Media, GIIII, Maternal serum
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
Comparison Between the Therapeutic Effects of Internal Sphincterotomy and Diltiazem Gel in Chronic Anal Fissure
143
150
FA
M.A.
Rajabi
Comparison Between the Therapeutic Effects of Internal Sphincterotomy and Diltiazem Gel in Chronic Anal Fissure M.A. Rajabi MD Received: 15/08/06 Sent for Revision: 18/10/06 Received Revised Manuscript: 15/05/07 Accepted: 29/05/07 Background and Objective: Surgery is now the "treatment of choice" for chronic anal fissure. However, considering the pathogenesis of this disease and the tendency for noninvasive and economical procedures, more attention is growing towards the non surgical treatments. Oral or topical nitroglycerin, isosorbide, bethanechol, L-Arginine, nifedipine, diltiazem, adrenergic –antagonists and botulinum toxin have been used to treat chronic anal fissure. In this study, we compared the surgical sphincterotomy and topical diltiazem gel for the treatment of anal fissure. Materials and Methods: This clinical trial study was performed on two groups of 35 patients. The first group was treated by surgical sphincterotomy and the second group received the topical diltiazem gel. Both groups were examined 2,4 and 6 weeks after the onset of the treatment and, the findings were recorded and statistically analyzed by SPSS, t-test and X2 to determine the relation between parameters and p<0.05 was considered to be significant. Results :Two weeks after the onset of treatment, the rate of complete pain relief in the sphincterotomy group and in the diltiazem group was 75% and 45% respectively (p<0.005). Although this value was the same in both groups 4 weeks after treatment (p=0.357), the wound healing process observed in the first group was significantly higher than the second group (85% VS 40%, p<0.0001). Both groups had a similar wound healing rate in the 6th week (p=0.0351). Conclusions: According to our findings, both the surgical sphinctorotomy and the topical diltiazem gel had a similar therapeutic effect on the chronic anal fissure. However, the topical diltiazem gel has shown to have several advantages such as lower complication rates, greater convenience, noninvassivess, (no hospitalization) and lower cost. Therefore this type of treatment considered to be more appropriate for chronic anal fissure, and the surgery should be used as an alternative option for special cases. Key words: Chronic anal fissure, Sphincterotomy, Diltiazem gel
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
6
2
2007
9
1
A Rare Anatomical Innervation of the Musculocutaneous Nerve
151
154
FA
M.M.
Taghavi
M.
Shariati Kohbanani
S.M.
Seyedmirzaee
A Rare Anatomical Innervation of the Musculocutaneous Nerve M.M. Taghavi MSc , M. Shariati Kohbanani MSc , S.M. Seyedmirzaee MD Received: 24/09/06 Sent for Revision: 20/02/07 Received Revised Manuscript: 07/04/07 Accepted: 27/05/07 Background and Objectives: Anatomically the musculocutaneous nerve (C5,6 ) is a branch of lateral cord of the brachial plexus and its motor nerve fibers innervates the muscles of anterior compartment of the arm. This nerve penetrates into the coracobrachialis of arm muscle and lies between biceps and brachialis muscles. At the lateral bicipital groove becomes superficial and the finally converts to lateral cutaneous nerve of the foream. Here we report a rare case of musculocutaneous nerve variation. Case Report: We found a rare anatomical form of musculocutaneous nerve during upper left limb dissection of a male cropse who was in dissecting room of Rafsanjan Medical School. His body was tall with muscular limbs, weighed 65-75Kg, 175 cm height, and fifty years old. The following variations were observed after dissecting of the axillary and arm regions. 1) The Musculocutaneous nerve arised from the lateral root of the median nerve. 2) The coracobrachialis muscle was innervated by a branch of the lateral cord of the brachial plexus. 3) The Musculocutaneous nerve did not penetrate into the coracobrachialis muscle but rather passed between the brachialis and biceps muscles. At the level of lateral bicipital groove, it then became a superficial nerve as the lateral cutaneous nerve of forearm. Hense, it was very close to the brachial artery and median nerve in the upper one-third of arm. Conclusion: This study describes a rare innervation of the musculocutanous nerve and requires further study to understand the nature of this unique structure. This atypical innervation is extremely important for surgical procedures performed on the arm muscles and adjecent vessels. Key words: Musculocutaneous nerve, Variation, Brachial plexus