Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Publication Ethics
251
252
FA
M.
Rezaeian
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Synthesis and In vitro Antibacterial Activity of N-[5-(5-nitroaryl)-1,3,4-Thiadiazol-2-yl] Piperazinyl Quinolones
253
262
FA
M.H.
Moshafi
S.M.
Safavi
A.
Foroumadi
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
The Effect of L-Carnitine and Heparin Supplementation on Plasma Glucose and Lactate Concentration During Exercise
263
272
FA
M.
Eizadi
A.
Eghdami
D.
Khorshidi
H.
Doali
F.
Kiani
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Prevalence of Hypertension and Its Association with the Anthropometric Indices in Adolescent Girls in Rafsanjan, 2007
273
286
FA
Z.
Salem
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
The Correlation of the Duration of Flaccidity Period and the Intensity and Location of the Disorder in Patients with Cerebro-Vascular Accident
287
294
FA
N.
Tahan
Kh.
Khademi Kalantari
Y.
Kholghi
Z.
Amiri
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Evaluation of Occult Hepatits B Virus Infection in Thalassemic Patients Infected by HCV in Kerman Province of Iran
295
302
FA
M.
Kazemi Arababadi
G.
Hassanshahi
M.
Rezaeian
E.
Rezazadeh Zarandi
R.
Vazirinejad
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Characterization of a Thermotolerant α-amylase Producing Natural Variant of Bacillus Species
303
316
OTHER
Background and Objectives: Thermotolerant α-amylases have had extensive commercial applications in starch processing, brewing and sugar production, and have almost completely replaced chemical hydrolysis of starch in the starch processing industry. The aim of this study was to determine the characterization of thermotolerant α-amylase producing natural variant of bacillus species
Materials and Methods: In this laboratory study, the isolated bacillus species known as Bacillus licheniformis Shahed-07 was identified by biochemical methods. The bacillus was cultured in liquid media to produce α-amylase. The enzyme production media were assessed controled and optimized for enzyme productivity using various carbon and nitrogen sources. The stability of the enzyme against temperature, pH, metal ions and chelating agents was then determined.
Results: Maximum enzyme production was achieved after 26 h cultivation at pH 7.0 and 50°C. Supplementation of medium with 0.5% Tryptophan enhanced the enzyme productivity to 202%, whereas Peptone and Lysin at 0.5% level showed a strong repression. In comparison with the crude α-amylase, the partially purified enzyme had 3.77 fold purity with 32.64 fold increased activity. This enzyme showed amylolytic activity with a single band of 6000 daltons on electrophoresis gel with 0.2% starch. The optimum activity was at pH 7.5 and 70°C. The crude enzyme was stable for 24 h at pH range of 6-7 at 70°C. Hg2+ was completely inhibitory to the enzyme activity, and Mn2+, Fe2+, Co2+ and Ca2+ increased the enzyme activity.
Conclusion: The B. licheniformis Shahed-07 strain produced high levels of thermotolerant α-amylase with characteristics suitable for application in starch processing and other food industries. The production process can be commercialized after further optimization for enhancing enzyme production.
Key word: Bacillus licheniformis, α-Amylase, Optimization, Production Medium
Conflict of interest: This research was funded by Shahed University.
Funding: None declared.
Ethical approval: The Ethical Committee of Shahed University approved the study.
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Comparison of Viable Human Sperm Count Using Three Different Methods Hypo-Osmotic Swelling Test, Viability/Cytotoxicity Assay, and Eosin-Nigrosin Technique: A Laboratory Study
317
326
FA
H.
Hakimi
Journal of Rafsanjan University of Medical Sciences
Journal of Rafsanjan University of Medical Sciences
1735-3165
8
4
2009
12
1
Solid-Pseudo-Papillary Tumor of Pancreas: A Case Report
327
334
OTHER
Background and Objectives: Solid Pseudo-Papillary Tumor (SPPT) is a benign and rare neoplasm which transforms to malignant from in fewer than 1 to 2 percent of cases. In more than 90% of the cases, it appears in young women ages 20 to 40 years old. Although SPPT is a benign tumor, it has special potential for mild malignancy.
Case Report: The patient was an 18-year old woman with abdominal mass, headache, fever, chill, decreased appetite, pallor and dysuria who referred to Shahid Sadooghi hospital of Yazd, Iran in July 2007. In examination, a firm inflated, mobile, and painless mass with distinct borders was palpated in the left upper abdominal quadrant. This mass was 16 cm in diameter. In CT scan a large hypodense mass with thick wall and crossing the center line on the right side of spine was prominent. Microscopic evaluations after the surgery showed tumoral cells of small polyhedral core elliptical, pseudopapillary processes trabeculs with diffused fibrosis, atrophy acinar and mild infiltration of chronic inflammatory cells in the tumors margins.
Conclusion: The final diagnosis in this patient was SPPT, a primary neoplasm of the pancreas. Its etiology is unknown and has low potential to turn into malignant form. It should be considered, especially in young women. Complete removal of the mass is the preferred treatment of the patients. Patients with local recurrence or metastasis can have long term survival after complete removal of the mass.
Key words: Solid – Pseudo Papilary Tumor, Gruber-Frantz Tumor, Pancreatic Tumors