Volume 12, Issue 5 (7-2013)                   JRUMS 2013, 12(5): 343-352 | Back to browse issues page

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Study of the Efects of Ruta Graveolens Aqueos Extract Effects on the Spermatogenesis of Mature Wistar Rat. JRUMS 2013; 12 (5) :343-352
URL: http://journal.rums.ac.ir/article-1-5295-en.html
Abstract:   (1608 Views)
 
Background and Objectives: Nowadays men hava accepted their role in pregnancy prevention. Some of these methods are non-hormonal. In non-hormonal methods plants are used. Ruta graveolens is one of these plants. This plant grows in Iran and was used as a contraceptive plant in the ancient medicine in both sexes. In this study the effect of aqueous extract of this plant on the time of rat spermatogenesis on testis tissue has been investigated.
Materials and Methods: In this experimental study 30 rats allocated randomly to three groups (n=10) as control which did not receive anything, vehicle which received only normal saline, and experimental which received ruta extract. Lethal dose 50 (LD50) extract was considered as 620 mg/kg. Based on this, sub LD 50 dose of aqueous extract 300mg/kg was injected intraperitoneally once every other day for 40 days. A day after last injection the animals were deeply anesthetized and dissected. The right testis were extruded and fixed for histological studies. For statistical analysis ANOVA and Tukey as a post hoc test were used.
Results: Intraperitoneal injection of 300mg/kg Ruta graveolens showed a significant decrease in the number of spermatogonia, primary spermatocyte, spermatid, lydig cell in experimental group as compared to control and vehicle. Also there were a significant increase in thickness of tunica albugina and a decrease in somniferous tubule diameter (p=0.01).
Conclusion: The aqueous extract of R.G diminishes the reproductive system activity and might be a useful substance for birth control process.
Key words: Aqueos extract, Ruta graveolens, Testis tissue, Rat, Spermatogenesis
 
Funding: This research was funded by physiology research center of Ahvaz Jundishapur University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Ahvaz Jundishapur University of Medical Sciences approved the study.
 
How to cite this article: Bazrafkan M, Panahi M, Ahangarpour A, Saki G. Study of the Efects of Ruta Graveolens Aqueos Extract Effects on the Spermatogenesis of Mature Wistar Rat. J Rafsanjan Univ Med Scie 2013; 12(5): 343-52. [Farsi]
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Type of Study: Research | Subject: Anatomy
Received: 2020/05/9 | Accepted: 2020/05/9 | Published: 2020/05/9

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