Background and Objective: Abnormal
regulation of apoptosis causes progressive pathological processes in the
placenta of pregnant women and affects on fetal growth. The aim of this study was to investige the effects of
endurance swimming training and silymarin
treatment during pregnancy on cadmium
exposure -induced liver apoptotic index in pregnant rats.
Materials and Methods: In this expremental study 72 female rats divided into 9
groups. Training program consisted of endurance
swimming, from the first day of pregnancy until delivery. Cadmium chloride
(CdCl2) was given as drinking water (400 mg/kg/l). Silymarin was
injected subcutaneously (100 mg/kg) 3 times a week. Apoptotic index was
determined by nonradioactive in situ end labeling method using TUNEL
immunocytochemical technique. The ANOVA and post hoc LSD (Least Significant
Difference) tests were used to analyze the findings of study.
Results: Cadmium significantly increased liver apoptotic index
(p=0.001). Swimming training induced no significant change in liver apoptotic
index (p=0.424). Swimming training and silymarin treatment significantly
decreased liver apoptotic index compared to cadmium (p=0.001).
Conclusion: Endurance swimming training in combination with silymarin treatment during
pregnancy can significantly counteract with cadmium-induced apoptotic index apoptosis in the liver of the pregnant
rats.
Keywords: Apoptotic index, Pregnant rat, Silymarin, Cadmium, Swimming
training
Funding: This research was funded by Mazandaran University,
Faculty of Physical Education and Sport Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of
Mazandaran University, Faculty of Physical Education and Sport Sciences,
approved the study.
How to cite this article: Mirdar Harijani Sh, Musavi N, Hamidian GhR, oliaei HA. Effect of
Endurance Swimming Training and Silymarin Treatment on Changes in Liver
Apoptotic Index in Pregnant Rats Exposed to Cadmium. J
RafsanjanUniv Med Sci 2014
13(8): 705-14. [Farsi]
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