Background
and Objective: Salubrinal-
a protein synthesis inhibitor- plays a crucial role in sleep and neuronal
inhibition through the GABA activation. According to these evidences, the aim
of the present study was to determine the effect of salubrinal on epileptic
seizures induced by pentylenetetrazol (PTZ).
Materials and Methods:
In this expremental study under stereotaxical surgery, three monopolar
electrodes were fixed on the skull bone of animals and a cannula was implanted
in the right cerebral ventricle according to Paxinos coordinates. After 7 days
of recovery, each animal received intracerebroventricular (ICV) vehicle,
diazepam (0.2 mg/rat) or salubrinal (25 or 50 µg/rat) 10 minutes before Pentylenetetrazol
injection (subcutaneous, 60mg/kg). Electrophysiological and behavioral
parameters of induced seizures were recorded during 60 minutes.
Results : Salubrinal (25
and 50 µg/rat)
significantly retarded the onset of generalized tonic-clonic seizures when
compared to vehicle treatment (p<0.05). Epileptic discharge duration and
ictal discharge duration were significantly shortened in salubrinal treated
rats (p<0.05). Additionally, the rats treated with salubrinal (25 and 50 µg/rat) had lower
seizure stage scores, as compared to vehicle treated rats (p<0.05).
Conclusion : The findings of
this study revealed that shortly after ICV. administration of salubrinal,
epileptic seizures can be significantly decreased. Also, the findings suggest
that protein synthesis is an essential process during development of epileptic seizures.
Key words : Seizure,
Salubrinal, Protein synthesis inhibitor, Rat, Pentylenetetrazol
Funding: This research was funded by
Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Conflict of interest: None declared.
Ethical approval: The Research Committee of Shahid
Sadoughi University of Medical Sciences approved the
study.
How to cite this article:
Izadi M, Rezvani ME, Esmailidehaj M. Effects of Intracerebroventricular Administration of
Salubrinal-A Protein Synthesis Inhibitor-on Epileptic Seizures Induced by
Pentylenetetrazol in Male Rats. J Rafsanjan Univ
Med Sci 2014
13(3): 281-92.
[Farsi]
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