Background and Objectives: Helicobacter pylori is a gram-negative and spiral bacterium that causes stomach and duodenal disease in humans. Because of the presence of disadvantages in antibiotic therapies, increasing efforts have been made to produce effective vaccine for this infection. The aim of this study was to generate a construct carrying the lnT gene and to survey its expression in human cells with RT-PCR method.Materials and Methods: In this laboratory study, lnT gene from the genome of Helicobacter pylori bacterial was isolated via polymerase chain reaction (PCR). Cloning of the PCR products was done by T/A cloning method in the appropriate T vector. Then, the lnT gene was subcloned into a pEGFP-C2 eukaryotic expression vector. To study the lnT gene expression, the final pEGFP-C2-lnT construct was transformed into human dermal fibroblasts (HDF) cells by electroporation and its expression was analyzed by RT-PCR.
[1]- MSc in Genetics, Dept. of Biology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
[2]- Associate Prof. of Molecular Genetics, Biotechnology Research Center, Dept. of Biology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
(Corresponding Author): Tel: (0383) 3361046, Fax:(0383)3361046, Email: abbasdoosti@yahoo.com
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