Volume 17, Issue 2 (5-2018)
JRUMS 2018, 17(2): 169-176 |
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Shojaei G ., Akhavan Sepahy A, Rafiei Tabatabae R, Tahvildari K. The Effects of Ethanolic Extract of Artemisia oliveriana on Expression of icaA, icaD, and ebps Genes in MRSA: A Short Report. JRUMS 2018; 17 (2) :169-176
URL: http://journal.rums.ac.ir/article-1-3976-en.html
URL: http://journal.rums.ac.ir/article-1-3976-en.html
Faculty of Basic Siences,North Tehran Branch,Islamic Azad University
Abstract: (3340 Views)
Background and Objectives: Expression of genes related to biofilm formation in methicillin-resistant staphylococcus aureus (MRSA) can play an effective role in biofilm formation and pathogenicity. Due to the significance of antimicrobial activity of Artemisia species against biofilm producer genes, this study aimed to evaluate the effect of ethanolic extract of Artemisia oliveriana on the expression of icaA, icaD, and ebps genes in MRSA.
Materials and Methods: This was a laboratory study. First, Artemisia oliveriana extract was prepared and then, the minimum inhibitory concentration (MIC) of growth for the extract of the plant against s. aureus strains containing mecA was obtained by microdilution method . Then, at the lower concentrations, levels of expression in the icaA, icaD, and ebps genes in MRSA were determined by Real-Time PCR, and the results were analyzed through repeated measures ANOVA.
Results: The MIC of ethanolic extract of A. oliveriana was 512 μg/ml. The reduction of the expression of the genes in the standard sample after 4, 8, and 16 hours was significant in the Artemisia oliveriana group (p<0.05).
Conclusion: The ethanolic extracts of Artemisia oliveriana had a significant effect on the expression of the genes involved in biofilm formation and this expression reduction was not seen in the housekeeping DNA gyrase-B as a reference gene.
Key words: Artemisia oliveriana, Biofilm, Real-Time PCR, Staphylococcus aureus, Methicillin
Funding: This study did not have any funds.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University, North Tehran Branch approved the study.
How to cite this article: Shojaei Gh, Akhavan Sepahy A, Rafiei Tabatabaei R, Tahvildari K. The Effects of Ethanolic Extract of Artemisia oliveriana on Expression of icaA, icaD, and ebps Genes in MRSA: A Short Report. Univ Med Sci 2018; 17 (2): 169-76. [Farsi]
Materials and Methods: This was a laboratory study. First, Artemisia oliveriana extract was prepared and then, the minimum inhibitory concentration (MIC) of growth for the extract of the plant against s. aureus strains containing mecA was obtained by microdilution method . Then, at the lower concentrations, levels of expression in the icaA, icaD, and ebps genes in MRSA were determined by Real-Time PCR, and the results were analyzed through repeated measures ANOVA.
Results: The MIC of ethanolic extract of A. oliveriana was 512 μg/ml. The reduction of the expression of the genes in the standard sample after 4, 8, and 16 hours was significant in the Artemisia oliveriana group (p<0.05).
Conclusion: The ethanolic extracts of Artemisia oliveriana had a significant effect on the expression of the genes involved in biofilm formation and this expression reduction was not seen in the housekeeping DNA gyrase-B as a reference gene.
Key words: Artemisia oliveriana, Biofilm, Real-Time PCR, Staphylococcus aureus, Methicillin
Funding: This study did not have any funds.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University, North Tehran Branch approved the study.
How to cite this article: Shojaei Gh, Akhavan Sepahy A, Rafiei Tabatabaei R, Tahvildari K. The Effects of Ethanolic Extract of Artemisia oliveriana on Expression of icaA, icaD, and ebps Genes in MRSA: A Short Report. Univ Med Sci 2018; 17 (2): 169-76. [Farsi]
Type of Study: Research |
Subject:
Microbiology
Received: 2017/09/26 | Accepted: 2018/02/18 | Published: 2018/05/14
Received: 2017/09/26 | Accepted: 2018/02/18 | Published: 2018/05/14
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