The Synergist Effects of Folic Acid and Vitamin B12 on Spatial Memory in Adult Male Rat Model of Alzheimer's Disease
A. Eskandary[1], A.A. Moazedi[2], N. Zamani[3]
Received: 26/09/2017 Sent for Revision:28/10/2017 Received Revised Manuscript: 16/12/2017 Accepted: 20/12/2017
Background and Objectives: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized clinically by progressive memory loss and subsequent dementia. The aim of this study was to evaluate the synergist effect of folic acid and vitamin B
12 on spatial memory in adult male rat Alzheimer's disease.
Material and methods: In this experimental study, 49 male Wistar rats were randomly divided into 7 groups: (7 rats in each group): control(intact), NBM lesion group, which received electrically- induced lesion (0.5 m A, 3s) in NBM, sham group ( the electrode was impaled in to the NBM with no lesion), NBM lesion + folic acid 5mg/kg, NBM lesion+ vitamin B
12 5mg/kg, NBM lesion+ folic acid- vitamin B
12, NBM lesion + saline. Acquisition and retention testing was done by using an eight-radial arm maze, in which the patterns of arm entries were recorded in each group for calculating working memory errors, reference memory error ,and latency. Data were analyzed using one-way ANOVA and Tukey's post hoc test.
Results: The results showed that there was a significant difference between the control and lesion groups in the parameters of the reference memory error, working memory error ,and elapsed time (P˂0.05). The administration of folic acid and vitamin B
12alone did not have any significant effect on spatial memory parameters compared with the lesion group. Co-administration folic acid- vitamin B
12 resulted in a reduction in the reference and working memory errors and the time spent in the eight-arm radial laser maze.
Conclusion: According to the results, the NMB lesion resulted in a reduction in spatial memory and co-administration of folic acid and vitamin B
12 resulted in improved spatial memory.
Key words: Alzheimer disease, Nucleus Basalis Magnocellularis, Folic acid, Vitamin B
12, Radial maze
Funding: This research was funded by Shahid Chamran University.
Conflict of interest: None declared.
Ethical approval: The Ethical Committee of Shahid Chamran University approved the study.
How to cite this article: Eskandary A, Moazedi A.A, Mamani N. The Synergist Effects of Folic Acid and Vitamin B
12 on Spatial Memory in Adult Male Rat Model of Alzheimer's Disease.
J Rafsanjan Univ Med Sci 2018; 16(10): 925-38. [Farsi]
- - PhD Student, Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
(Corresponding Author) Tel: (061)33331045, Fax: (061)33331045, E-mail: azade.eskandary@gmail.com
- - Professor, Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
- - PhD Student, Department of Biology, Faculty of Sciences, Payam-e-noor University, Tehran , Iran