The Effect of Sodium Valproate and Low Frequency Electrical Stimulation on Motor Activity During Rapid Kindling of Hippocampal CA1 Region in Adult Male Rat: An Experimental Study
R[j1] . Zalkhani[1], A. A. Moazedi[2], Z. Ghotbeddin[3], M. Pourmahdi Borujeni[4]
Received: 15/10/2018 Sent for Revision: 17/10/2018 Received Revised Manuscript: 15/12/2018 Accepted: 19/12/2018
Background and Objectives: Epilepsy is a chronic neurological disorder that requires long treatment with less side effects. Therefore, this study aimed to determine the effect of sodium valproate and electrical stimulation of the brain with low frequency (LFS) on the motor activity of male rat during hippocampus rapid kindling.
Material and Methods: In this experimental study, 56 male adult rats were randomly divided to 8 groups: control, sham operation, saline-kindled and drug-kindled groups which received saline or drug 15 minutes before kindling stimulations and saline-kindled-LFS or drug-kindled-LFS group which received saline or drug 15 minutes before kindling stimulations and LFS after termination of kindling stimulations. Then animals motor activity was assessed on the 6
th day by open field test and data were analyzed by one-way ANOVA and Tukey's post-test.
Results: The open field results showed a significant increase in the number of motions (p =0.007) in the kindled group compared to the sham group. But these parameters
showed no
difference in saline-kindled-LFS group, drug-kindled and drug-kindled-LFS groups
compared with the
sham group (p˃0.05).
Conclusion: It seems administration of sodium valproate and application of low-frequency electrical to hippocampus region can attenuate the kindling-induced hyperactivity in adult male rat.
Key words: Sodium valproate, Low frequency electrical stimulation, Open field, Kindling, Rat
Funding: This research was funded by Shahid Chamran University of Ahvaz.
Conflict of interest: None declared.
Ethical approval: The Ethical Committee of Shahid Chamran University of Ahvaz approved the study (EE/97.3.24.49755/scu.ac.ir).
How to cite this article: Zalkhani R, Moazedi AA, Ghotbeddin Z, Pourmahdi Borujeni M. The Effect of Sodium Valproate and Low Frequency Electrical Stimulation on Motor Activity During Rapid Kindling of Hippocampal CA1 Region in Adult Male Rat: An Experimental Study
. J Rafsanjan Univ Med Sci 2019; 18 (1): 43-54. [Farsi]
- - PhD Student of Physiology, Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
ORCID: 0000-0003-2708-5254
(Corresponding Author) Tel: (061) 33330015, Fax: (061) 33360807, E-mail:
ghotbeddiny2010@gmail.com
[2] - Prof. of Physiology, Dept. of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
ORCID: 0000-0003-1346-2426
[3] - Assistant Prof. of Physiology, Dept. of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran, ORCID: 0000-0003-2110-6333
[4] - Associate Prof. of Epidemiology, Dept. of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran, ORCID: 0000-0002-9478-260X
[j1]مشخصات نویسنده مسئول مطابق با فرمت مجله اضافه گردد.