Volume 21, Issue 1 (4-2022)
JRUMS 2022, 21(1): 49-70 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khodadoost M, Shakeryan S, Arjmand S, Nikbakht M. The Effect of High and Low-Intensity Interval Training on TRF1 and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study. JRUMS 2022; 21 (1) :49-70
URL: http://journal.rums.ac.ir/article-1-6277-en.html
URL: http://journal.rums.ac.ir/article-1-6277-en.html
Sahid Chamran University of Ahvaz
Full-Text [PDF 500 kb]
(494 Downloads)
| Abstract (HTML) (925 Views)
Full-Text: (811 Views)
The Effect of High and Low-Intensity Interval Training on TRF1 [j1] and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study
Mostafa Khodadoost[1], Saead Shakeryan[2], Sareh Arjmand[3], Masood Nikbakht[4]
Received: 09/11/21 Sent for Revision: 19/12/20 Received Revised Manuscript: 02/01/22 Accepted: 05/01/22
Background and Objectives: The process of chronic diseases and aging is associated with reduced telomere length. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and low-intensity interval training (LIIT) on telomere repeat binding factor 1 and 2 (TRF1 and TRF2) in Soleus (SOL) muscle as a slow-twitch (ST) and Extensor Digitorum Longus (EDL) muscle as a fast-twitch (FT) of mice skeletal muscle.
Materials and Methods: In this experimental study, the subjects were C57BL/6 mice (n=24) that were randomly divided into four groups of 6 mice: 1-Base control, 2-control, 3-high-intensity interval training (HIIT), and 4-low-intensity interval training (LIIT). The exercise included 5 days a week for 8 weeks. The gene expression of muscle tissues was measured using RT-PCR. The data were evaluated using two-way ANOVA with Tukey’s post hoc test.
Results: By comparing ST and FT muscles (p=0.791) and HIIT and LIIT groups (p=0.164) and also the interaction between muscle type and training intensity (p=0.929), there was no significant difference in the expression of TRF1 gene. There was no significant interaction between TRF2 gene expression in ST and FT muscles at different intensities (p=0.868). But the results of Tukey’s post hoc test showed that in terms of TRF2 gene expression, HIIT (p=0.031) and LIIT (p=0.035) groups showed more increase compared to the control group.
Conclusion: HIIT and LIIT similarly improved the telomere system in skeletal muscle of both muscle types, and this system was more affected by telomere elongation effect of TRF2.
Key words: Interval training, ST and FT skeletal muscles, TRF1, TRF2
Funding: This study was funded by Shahid Chamran University of Ahvaz.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Jundishapur University of Medical Sciences approved the study (EE/98.24.3.26525/scu.ac.ir).
How to cite this article: Mostafa Khodadoost, Saead Shakeryan, Sareh Arjmand, Masood Nikbakht. The Effect of High and Low-Intensity Interval Training on TRF1 and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study. J Rafsanjan Univ Med Sci 2022; 21 (1): 49-70. [Farsi]
Mostafa Khodadoost[1], Saead Shakeryan[2], Sareh Arjmand[3], Masood Nikbakht[4]
Received: 09/11/21 Sent for Revision: 19/12/20 Received Revised Manuscript: 02/01/22 Accepted: 05/01/22
Background and Objectives: The process of chronic diseases and aging is associated with reduced telomere length. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and low-intensity interval training (LIIT) on telomere repeat binding factor 1 and 2 (TRF1 and TRF2) in Soleus (SOL) muscle as a slow-twitch (ST) and Extensor Digitorum Longus (EDL) muscle as a fast-twitch (FT) of mice skeletal muscle.
Materials and Methods: In this experimental study, the subjects were C57BL/6 mice (n=24) that were randomly divided into four groups of 6 mice: 1-Base control, 2-control, 3-high-intensity interval training (HIIT), and 4-low-intensity interval training (LIIT). The exercise included 5 days a week for 8 weeks. The gene expression of muscle tissues was measured using RT-PCR. The data were evaluated using two-way ANOVA with Tukey’s post hoc test.
Results: By comparing ST and FT muscles (p=0.791) and HIIT and LIIT groups (p=0.164) and also the interaction between muscle type and training intensity (p=0.929), there was no significant difference in the expression of TRF1 gene. There was no significant interaction between TRF2 gene expression in ST and FT muscles at different intensities (p=0.868). But the results of Tukey’s post hoc test showed that in terms of TRF2 gene expression, HIIT (p=0.031) and LIIT (p=0.035) groups showed more increase compared to the control group.
Conclusion: HIIT and LIIT similarly improved the telomere system in skeletal muscle of both muscle types, and this system was more affected by telomere elongation effect of TRF2.
Key words: Interval training, ST and FT skeletal muscles, TRF1, TRF2
Funding: This study was funded by Shahid Chamran University of Ahvaz.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Jundishapur University of Medical Sciences approved the study (EE/98.24.3.26525/scu.ac.ir).
How to cite this article: Mostafa Khodadoost, Saead Shakeryan, Sareh Arjmand, Masood Nikbakht. The Effect of High and Low-Intensity Interval Training on TRF1 and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study. J Rafsanjan Univ Med Sci 2022; 21 (1): 49-70. [Farsi]
[1]- PhD Student in Sport Physiology, Dept. of Sport Physiology, Faculty of Sports Sciences, Sahid Chamran University of Ahvaz, Ahvaz, Iran
[2]- Associated Prof., Dept. of Sport Physiology, Faculty of Sports Sciences, Sahid Chamran University of Ahvaz, Ahvaz, Iran
ORCID: 0000-0002-0880-9942
(Corresponding Author) Tel: (061) 53360111, Fax: (061) 53360111, E-mail: sashakeryan@gmail.com
ORCID: 0000-0002-0880-9942
(Corresponding Author) Tel: (061) 53360111, Fax: (061) 53360111, E-mail: sashakeryan@gmail.com
[3]- Assistant Prof., Protein Research Center, Shahid Beheshti University of Tehran, Tehran, Iran
[4]- Associated Prof., Dept. of Sport Physiology, Faculty of Sports Sciences, Sahid Chamran University of Ahvaz, Ahvaz, Iran
Type of Study: Research |
Subject:
تربيت بدني
Received: 2021/11/5 | Accepted: 2022/01/5 | Published: 2022/04/21
Received: 2021/11/5 | Accepted: 2022/01/5 | Published: 2022/04/21
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
