Volume 21, Issue 2 (5-2022)
JRUMS 2022, 21(2): 207-220 |
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Nikasa M, Noruzinia M, Farshdousti Hagh M. Evaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study. JRUMS 2022; 21 (2) :207-220
URL: http://journal.rums.ac.ir/article-1-6327-en.html
URL: http://journal.rums.ac.ir/article-1-6327-en.html
Keywords: Osteoblast, Mesenchymal stem cells, Differentiation, Global DNA methylation, Zoledronic acid
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Evaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study
Mina Nikasa[1], Mehrdad Noruzinia[2], Majid Farshdousti Hagh[3]
Received: 11/12/21 Sent for Revision: 04/01/22 Received Revised Manuscript: 24/04/22 Accepted: 25/04/22
Background and Objectives: Control processes in osteoblastic differentiation of mesenchymal stem cells are not yet fully understood. Epigenetic mechanisms, especially the methylation of CpG Islands in the promoter of genes, are considered as one of the most important control mechanisms in stem cell differentiation. In the process of differentiation, it is debated whether only the methylation of specific genes changes or the methylation of global DNA. Therefore, in the present study, the state of global DNA methylation was evaluated during osteoblastic differentiation of mesenchymal stem cells by differentiation medium and also in treatment by zoledronic acid.
Materials and Methods: In this laboratory study, after isolation and proliferation of mesenchymal stem cells, induction of osteoblastic differentiation was done using differentiating medium and zoledronic acid. DNA extraction was performed at differentiation weeks 1, 2, and 3 as well as from undifferentiated mesenchymal stem cells. Global DNA methylation status was assessed by antibodies against 5-methyl cytosine. Repeated measurement design test was used to analyze the data.
Results: Global DNA methylation status of the genome did not change during osteoblastic differentiation of mesenchymal stem cells (p=0.093). Also, treatment with zoledronic acid had no effect on global DNA methylation (p=0.057).
Conclusion: Osteoblastic differentiation of mesenchymal stem cells by differentiation medium and also in treatment with zoledronic acid is not associated with altered global methylation of the genome. Therefore, the differentiation process of mesenchymal stem cells to osteoblasts is a unique pathway, and possibly other genetic and epigenetic mechanisms play a role in controlling it.
Key words: Osteoblast, Mesenchymal stem cells, Differentiation, Global DNA methylation, Zoledronic acid
Funding: This study was funded by Tabriz University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Tabriz University of Medical Sciences approved the study (IR.TBZMED.VCR.REC.1398.242).
How to cite this article: Nikasa Mina, Noruzinia Mehrdad, Farshdousti Hagh Majid. Evaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study. J Rafsanjan Univ Med Sci 2022; 21 (02): 207-20. [Farsi]
Mina Nikasa[1], Mehrdad Noruzinia[2], Majid Farshdousti Hagh[3]
Received: 11/12/21 Sent for Revision: 04/01/22 Received Revised Manuscript: 24/04/22 Accepted: 25/04/22
Background and Objectives: Control processes in osteoblastic differentiation of mesenchymal stem cells are not yet fully understood. Epigenetic mechanisms, especially the methylation of CpG Islands in the promoter of genes, are considered as one of the most important control mechanisms in stem cell differentiation. In the process of differentiation, it is debated whether only the methylation of specific genes changes or the methylation of global DNA. Therefore, in the present study, the state of global DNA methylation was evaluated during osteoblastic differentiation of mesenchymal stem cells by differentiation medium and also in treatment by zoledronic acid.
Materials and Methods: In this laboratory study, after isolation and proliferation of mesenchymal stem cells, induction of osteoblastic differentiation was done using differentiating medium and zoledronic acid. DNA extraction was performed at differentiation weeks 1, 2, and 3 as well as from undifferentiated mesenchymal stem cells. Global DNA methylation status was assessed by antibodies against 5-methyl cytosine. Repeated measurement design test was used to analyze the data.
Results: Global DNA methylation status of the genome did not change during osteoblastic differentiation of mesenchymal stem cells (p=0.093). Also, treatment with zoledronic acid had no effect on global DNA methylation (p=0.057).
Conclusion: Osteoblastic differentiation of mesenchymal stem cells by differentiation medium and also in treatment with zoledronic acid is not associated with altered global methylation of the genome. Therefore, the differentiation process of mesenchymal stem cells to osteoblasts is a unique pathway, and possibly other genetic and epigenetic mechanisms play a role in controlling it.
Key words: Osteoblast, Mesenchymal stem cells, Differentiation, Global DNA methylation, Zoledronic acid
Funding: This study was funded by Tabriz University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Tabriz University of Medical Sciences approved the study (IR.TBZMED.VCR.REC.1398.242).
How to cite this article: Nikasa Mina, Noruzinia Mehrdad, Farshdousti Hagh Majid. Evaluation of Changes in Global DNA Methylation during Osteoblastic Differentiation of Mesenchymal Stem Cells: A Laboratory Study. J Rafsanjan Univ Med Sci 2022; 21 (02): 207-20. [Farsi]
[1]- MSc, Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
[2]- Associate Prof., Dept. of Hematology, Tarbiat Modares University, Tehran, Iran
[3]- Associate Prof., Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, ORCID: 0000-0002-2340-6594
(Corresponding Author) Tel: (041) 33364665, Fax: (041) 33364664, E-mail: m.farshdousti@gmail.com
(Corresponding Author) Tel: (041) 33364665, Fax: (041) 33364664, E-mail: m.farshdousti@gmail.com
Type of Study: Research |
Subject:
زيست شناسي
Received: 2021/12/6 | Accepted: 2022/04/25 | Published: 2022/05/20
Received: 2021/12/6 | Accepted: 2022/04/25 | Published: 2022/05/20
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