Volume 22, Issue 12 (2-2024)
JRUMS 2024, 22(12): 1335-1346 |
Back to browse issues page
Ethics code: IR.RUMS.REC.1397.079
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Kaeidi A, Hassanshahi J. Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin:
An Experimental Study. JRUMS 2024; 22 (12) :1335-1346
URL: http://journal.rums.ac.ir/article-1-7121-en.html
URL: http://journal.rums.ac.ir/article-1-7121-en.html
Rafsanjan University of Medical Sciences
Abstract: (198 Views)
Background and Objectives: Ureteral obstruction is one of the causes of kidney damage. Troxerutin is one of the strong natural antioxidants. The purpose of this study was to investigate the levels of oxidative stress and inflammation in the right kidney tissue after the left ureteral obstruction in male rats.
Materials and Methods: In this experimental study, 18 male Wistar rats were randomly divided into 3 groups (n=6 in either group) including control, ureteral obstruction, and ureteral obstruction+troxerutin. After the left ureter occlusion for 3-day, rats received troxerutin (100 mg/kg) daily 1h after occlusion for three days. Then, the right kidney weight and the blood urea nitrogen (BUN) level were measured. The malondialdehyde concentration, the glutathione peroxidase, and superoxide dismutase enzyme activity levels, as well as the tumor necrosis factor-alpha (TNF-α) expression level, were measured in the right kidney tissue. One-way ANOVA and Tukey's post hoc test were used to analyze the data.
Results: Left ureteral obstruction caused a significant increase in the right kidney weight, malondialdehyde concentration, and TNF-α expression level (P=0.002) and a decrease in the activity levels of glutathione peroxidase (P=0.005) and superoxide dismutase (P=0.009) enzymes of ureteral obstruction group versus the control. Troxerutin was able to ameliorate these indicators in the ureteral obstruction+troxerutin group compared to the ureteral obstruction group (P<0.05). Blood urea nitrogen level mean did not show significant differenceacross the groups (P>0.05).
Conclusion: Left ureter obstruction induces inflammation and oxidative stress in the right kidney, and troxerutin consumption reduces inflammation and oxidative stress levels.
Key words: Ureteral obstruction, Kidney, Oxidative stress, Inflammation, Troxerutin, Rat
Funding: This study was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1397.079).
How to cite this article: Kaeidi A, Hassanshahi J. Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin: An Experimental Study. J Rafsanjan Univ Med Sci 2024; 22(12): 1335-46. [Farsi]
Materials and Methods: In this experimental study, 18 male Wistar rats were randomly divided into 3 groups (n=6 in either group) including control, ureteral obstruction, and ureteral obstruction+troxerutin. After the left ureter occlusion for 3-day, rats received troxerutin (100 mg/kg) daily 1h after occlusion for three days. Then, the right kidney weight and the blood urea nitrogen (BUN) level were measured. The malondialdehyde concentration, the glutathione peroxidase, and superoxide dismutase enzyme activity levels, as well as the tumor necrosis factor-alpha (TNF-α) expression level, were measured in the right kidney tissue. One-way ANOVA and Tukey's post hoc test were used to analyze the data.
Results: Left ureteral obstruction caused a significant increase in the right kidney weight, malondialdehyde concentration, and TNF-α expression level (P=0.002) and a decrease in the activity levels of glutathione peroxidase (P=0.005) and superoxide dismutase (P=0.009) enzymes of ureteral obstruction group versus the control. Troxerutin was able to ameliorate these indicators in the ureteral obstruction+troxerutin group compared to the ureteral obstruction group (P<0.05). Blood urea nitrogen level mean did not show significant differenceacross the groups (P>0.05).
Conclusion: Left ureter obstruction induces inflammation and oxidative stress in the right kidney, and troxerutin consumption reduces inflammation and oxidative stress levels.
Key words: Ureteral obstruction, Kidney, Oxidative stress, Inflammation, Troxerutin, Rat
Funding: This study was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1397.079).
How to cite this article: Kaeidi A, Hassanshahi J. Levels of Oxidative Stress and Inflammation in the Right Kidney After Left Ureteral Obstruction in Male Rats Treated With Troxerutin: An Experimental Study. J Rafsanjan Univ Med Sci 2024; 22(12): 1335-46. [Farsi]
Type of Study: Research |
Subject:
Physiology
Received: 2023/08/31 | Accepted: 2024/02/4 | Published: 2024/02/4
Received: 2023/08/31 | Accepted: 2024/02/4 | Published: 2024/02/4
References
1. Reicherz A, Eltit F, Almutairi K, Mojtahedzadeh B, Herout R, Chew B, et al. Ureteral obstruction promotes ureteral inflammation and fibrosis. Eur Urol Focus 2023; 9(2): 371-80.
2. Merindol I, Vachon C, Juette T, Dunn M. Benign ureteral obstruction in cats: Outcome with medical management. JVIM 2023; 37(3): 1047-58.
3. Tabib C, Nethala D, Kozel Z, Okeke Z. Management and treatment options when facing malignant ureteral obstruction. International Journal of Urology 2020; 27(7): 591-8.
4. Hassanshahi J, Maleki M, Nematbakhsh M. Renal blood flow and vascular resistance responses to angiotensin II in irreversible and reversible unilateral ureteral obstruction rats; the role of angiotensin II type 1 and 2 receptors. Journal of Nephropathology 2018; 7(2).
5. Goorani S, Khan AH, Mishra A, El-Meanawy A, Imig JD. Kidney Injury by Unilateral Ureteral Obstruction in Mice Lacks Sex Differences. Kidney Blood Press Res 2024; 49(1): 69-80.
6. Ma X, Chang Y, Xiong Y, Wang Z, Wang X, Xu Q. Eplerenone Ameliorates Cell Pyroptosis in Contralateral Kidneys of Rats with Unilateral Ureteral Obstruction. Nephron 2019; 142(3): 233-42.
7. Al Zuhairi JJ, Kashi FJ, Rahimi-Moghaddam A, Yazdani M. Antioxidant, cytotoxic and antibacterial activity of Rosmarinus officinalis L. essential oil against bacteria isolated from urinary tract infection. Eur J Integr Med 2020; 38: 101192.
8. Rahmani M, Kaeidi A, Hassanshahi J. The effect of left unilateral ureteral obstruction on contralateral renal antioxidant system changes in male rats: with an emphasis on the protective role of Oleuropein. JJUMS 2021; 8(2): 613-21.
9. de Lima Cherubim DJ, Buzanello Martins CV, Oliveira Fariña L, da Silva de Lucca RA. Polyphenols as natural antioxidants in cosmetics applications. J Cosmet Dermatol 2020; 19(1): 33-7.
10. Kiani R, Arzani A, Mirmohammady Maibody SA. Polyphenols, flavonoids, and antioxidant activity involved in salt tolerance in wheat, Aegilops cylindrica and their amphidiploids. Front Plant Sci 2021; 12: 646221.
11. Vidhya R, Anuradha CV. Anti-inflammatory effects of troxerutin are mediated through elastase inhibition. Immunopharmacol Immunotoxicol 2020; 42(5): 423-35.
12. Bahrami A, Hassanpour S, Zendehdel M. Effect of troxerutin consumption during gestation period on reflexive motor behavior in mice offspring. Int J Dev Neurosci 2023; 83(3): 248-56.
13. Ahmadi Z, Mohammadinejad R, Roomiani S, Afshar EG, Ashrafizadeh M. Biological and therapeutic effects of troxerutin: molecular signaling pathways come into view. JoP 2021; 24(1): 1.
14. Hassanshahi J, Mirzahosseini A, Hajializadeh Z, Kaeidi A. Anticancer and cytotoxic effects of troxerutin on HeLa cell line: an in-vitro model of cervical cancer. Mol Biol Rep 2020; 47: 6135-42.
15. Dadpisheh S, Ahmadvand H, Jafaripour L, Nouryazdan N, Babaeenezhad E, Shati H, et al. Effect of troxerutin on oxidative stress induced by sciatic nerve ischemia-reperfusion injury. JKUMS 2020; 27(4): 338-47.
16. Malinska H, Hüttl M, Oliyarnyk O, Markova I, Poruba M, Racova Z, et al. Beneficial effects of troxerutin on metabolic disorders in non-obese model of metabolic syndrome. PLoS One 2019; 14(8): e0220377.
17. Zamanian M, Bazmandegan G, Sureda A, Sobarzo-Sanchez E, Yousefi-Manesh H, Shirooie S. The protective roles and molecular mechanisms of troxerutin for the treatment of chronic diseases: A mechanistic review. Current Neuropharmacology 2021; 19(1): 97-110.
18. Tantawy MN, Jiang R, Wang F, Takahashi K, Peterson TE, Zemel D, et al. Assessment of renal function in mice with unilateral ureteral obstruction using 99m Tc-MAG3 dynamic scintigraphy. BMC Nephrology 2012; 13(1): 1-11.
19. Kaeidi A, Taghipour Z, Allahtavakoli M, Fatemi I, Hakimizadeh E, Hassanshahi J. Ameliorating effect of troxerutin in unilateral ureteral obstruction induced renal oxidative stress, inflammation, and apoptosis in male rats. Naunyn-Schmiedeberg's Archives of Pharmacology 2020; 393: 879-88.
20. Najafizadeh A, Kaeidi A, Rahmani M, Hakimizadeh E, Hassanshahi J. The protective effect of carvacrol on acetaminophen-induced renal damage in male rats. Mol Biol Rep 2022; 49(3): 1763-71.
21. Hassanshahi J, Shibani F, Kaeidi A. The Oleuropein Effect on Neurotrophic Factor Expression Level in Hippocampal Tissue and Memory Defect Induced by Morphine in Male Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19(9): 969-78.
22. Kaeidi A, Sahamsizadeh A, Allahtavakoli M, Fatemi I, Rahmani M, Hakimizadeh E, et al. The effect of oleuropein on unilateral ureteral obstruction induced-kidney injury in rats: the role of oxidative stress, inflammation and apoptosis. Mol Biol Rep 2020; 47: 1371-9.
23. Watatani H, Maeshima Y, Hinamoto N, Yamasaki H, Ujike H, Tanabe K, et al. Vasohibin‐1 deficiency enhances renal fibrosis and inflammation after unilateral ureteral obstruction. Physiological Reports 2014; 2(6): e12054.
24. Adam B, Pentz R, Siegers C, Strubelt O, Tegtmeier M. Troxerutin protects the isolated perfused rat liver from a possible lipid peroxidation by coumarin. Phytomedicine 2005; 12(1-2): 52-61.
25. Elangovan P, Pari L. Ameliorating effects of troxerutin on nickel-induced oxidative stress in rats. Redox Report 2013; 18(6): 224-32.
26. Badalzadeh R, Layeghzadeh N, Alihemmati A, Mohammadi M. Beneficial effect of troxerutin on diabetes-induced vascular damages in rat aorta: histopathological alterations and antioxidation mechanism. Int J Endocrinol Metab 2015; 13(2).
27. Shan Q, Zhuang J, Zheng G, Zhang Z, Zhang Y, Lu J, et al. Troxerutin Reduces Kidney Damage against BDE-47-Induced Apoptosis via Inhibiting NOX2 Activity and Increasing Nrf2 Activity. Oxid Med Cell Longev 2017; 2017.
28. Misseri R, Meldrum DR, Dagher P, Hile K, Rink RC, Meldrum KK. Unilateral ureteral obstruction induces renal tubular cell production of tumor necrosis factor-α independent of inflammatory cell infiltration. The Journal of Urology 2004; 172(4): 1595-9.
29. Badalzadeh R, Baradaran B, Alihemmati A, Yousefi B, Abbaszadeh A. Troxerutin preconditioning and ischemic postconditioning modulate inflammatory response after myocardial ischemia/reperfusion injury in rat model. Inflammation 2017; 40: 136-43.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
