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Volume 6, Issue 3 (12-2007)
Abstract

Effect of Extraction Method on Antimicrobial Properties of Shoot Extract of Purple Coneflower (Echinacea Purpurea l.) Against Some Pathogenic Bacteria
Z. Izadi, A. Sorooshzadeh, S.a. Modarres Sanavi, M. Esna-Ashari, M. Aghaalikhani, P. Davoodi,
Volume 13, Issue 3 (10-2014)
Abstract

Background and Objective: In recent years, it is recommended to use natural materials like plant extracts and essences instead of chemical preservatives in food industry. The main objective of this study was to evaluate the antimicrobial activity of shoot extract of purple coneflower against some gram positive and gram negative bacteria. 

Materials and Methods: This Laboratory study was conducted in 2013. Two extraction methods (maceration and microwave-assisted) with three solvents (water, methanol 80% and acetone) were used to extract the phenolic compounds from purple coneflower shoot The micro-organisms investigated in this study were: Bacillus cereus, Listeria monocytogenes, Escherichia coli, Shigella dysenteriae and Pseudomonas aeruginosa. Experimental data were analyzed using ANOVA by the SPSS version 15 software and mean comparison were done using the Duncan's multiple range test.

Results: The highest total phenolic content was related to the methanol extract produced by microwave-assisted extraction (MAE), whereas in both extraction methods, the lowest amount of phenolic was obtained by using acetone extract. Comparing the extraction methods showed that MAE had higher extraction efficiency in all three tested solvents. Regarding to antimicrobial activity of purple coneflower shoot extracts, the minimum inhibitory concentration and minimum bactericidal concentration were observed in gram positive bacteria (Bacillus cereus and Listeria monocytogenes). Pseudomonas aeruginosa was the most resistant bacterium against the extracts.

Conclusion: The results showed that in most cases, the extracts obtained by MAE method had more antimicrobial activity in comparison to traditional method. In addition the microwave-assisted extract of this plant can be considered as a food preservative. However, more studies, such as examinations in food models are needed to unravel the antimicrobial effects of given plant.

Key words: Purple coneflower extract, Antimicrobial properties, Extraction method.

Funding: This research was funded by Hamadan University of Medical Sciences and Tarbiat Modares University.

Conflict interest: None declared.

Ethical approval: This article does not need permission from the Ethics Committee because the information in this article was derived from a non-animal research.

 

How to cite this article: Izadi Z, Sorooshzadeh A, Modarres Sanavi S.A.M, Esna-Ashari M, AghaAlikhani M, Davoodi P. Effect of Extraction Method on Antimicrobial Properties of Shoot Extract of Purple Coneflower (Echinacea Purpurea l.) Against Some Pathogenic Bacteria?. J Rafsanjan Univ Med Sci 2014 13(3): 267-80. [Farsi]


Prevalence of Clindamycin Inducible Resistance in Staphylococcus Aureus and Coagulase-Negative Staphylococci Using D-Test: A Short Report
Gh.r. Goudarzi, M. Azadpour,
Volume 14, Issue 1 (4-2015)
Abstract

Background and Objective: The D-test is performed by placing clindamycin and erythromycin impregnated disks at a standard distance on the agar plate then looking for the flattening of inhibition zone around the clindamycin disk. A positive D-test indicates clindamycin inducible resistance and also predicts treatment failure possibility of this antibiotic in clinic. Prevalence of staphylococcal inducible resistance to clindamycin through the D-test was the aim of this study.

Materials and Methods: In this descriptive-cross-sectional study, inducible resistance to clindamycin was examined on the 150 isolated Staphylococcus aureus (n=103) and coagulase-negative staphylococci (n=47) collected from nasal employees and clinical samples by using D-test. The statistical analysis was performed using chi-square test.

Results: Out of 103 isolated S. aureus, 22 (21.4%) were methicillin-resistant (MRSA). Among the tested samples, 3(2%), 1(0.6%) and 2(1.3%) had D, D+ and HD phenotypes, respectively. Fifty-four (36%) of isolations exhibited resistance to both clindamycin and erythromycin. Prevalence of resistance to clindamycin and erythromycin between isolated MRSA and methicillin-susceptible S. aureus (MSSA) was statistically significant (p<0.05).

Conclusion: Phenotypical resistance to erythromycin is not always attributable to clindamycin resistance. Therefore, staphylococcus strains resistant to erythromycin and susceptible to clindamycin should be subjected to D-test assay.

Key words: Inducible resistance, Clindamycin, Staphylococcus aureus, D-test

 

Funding: This research was funded by Lorestan University of Medical Sciences.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Lorestan University of Medical Sciences approved the study.

 

How to cite this article: Goudarzi Gh.R, Azadpour M. Prevalence of Clindamycin Inducible Resistance in Staphylococcus Aureus and Coagulase-Negative Staphylococci Using D-Test: A Short Report. J RafsanjanUniv Med Sci 2015 14(1): 77-82. [Farsi]


The Effect of Salvia shariffi and Salvia virgata Hydroalcoholic Extracts on Some Serum Biochemical Parameters in Male Hyperlipidemic Rats
L. Shahraki Mojahed, S.a. Davari, M.r. Hajinezhad,
Volume 16, Issue 5 (9-2017)
Abstract

Background and Objectives: Hyperlipidemia is a progressive worldwide metabolic disorder. The aim of the present study was evaluation of effects of Salvia shariffi and Salvia virgata hydroalcoholic extracts on some serum biochemical parameters in hyperlipidemic male rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were randomly divided into 4 equal groups including control, hyperlipidemic rats, and hyperlipidemic rats treated with S. virgata and S. shariffi hydroalcoholic extracts (200 mg/kg bw). High-fat diet was administered to rats for one month. Then, hyperlipidemia was confirmed by measurement of serum triglyceride and cholesterol levels and the extracts were orally treated to the groups for 21 days. Finally, serum cholesterol and triglyceride levels and hepatic enzymes were measured and the obtained data were analyzed using one-way ANOVA and Tukey's post hoc test.
Results: Comparing to the hyperlipidemic group, the serum levels of cholesterol, triglyceride, and low-density lipoprotein were significantly reduced in the treated groups but this reduction was brilliant in the group treated with S. shariffi (P<0.05). Also, both extracts were able to increase the high-density lipoprotein level but it was only significant in the group treated with S. shariffi (P<0.05). Moreover, serum levels of aspartate aminotransferase and alanine aminotransferase were significantly decreased in the treated groups comparing to the hyperlipidemic group (P<0.05 and P<0.01, respectively) but this reduction was not significant between the treated groups (P˃0.05).
Conclusion: Both extracts could improve lipid profile and liver enzymes in hyperlipidemic rats but this improvement was more pronounced in the S. shariffi treated group.
Key words: Salvia shariffi, Salvia virgata, Rat, Hyperlipidemia, Liver enzymes
 
Funding: This research was funded by University of Zabol.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of University of Zabol approved the study (UOZ.ECRA.2017.2316905).
How to cite this article: Shahraki Mojahed L, Davari SA, Hajinezhad MR. The Effect of Salvia shariffi and Salvia virgata Hydroalcoholic Extracts on some Serum Biochemical Parameters in Male Hyperlipidemic Rats. J Rafsanjan Univ Med Sci 2017; 16(5): 437-48. [Farsi]
 


The Effect of Calendula, Aloe and Caster on Cutaneous Wounds Healing Process in Mature Male Rat
S.a. Davari, M.r. Hajinezhad, K. Samadi, S. Eftekhari,
Volume 17, Issue 2 (5-2018)
Abstract
Background and Objectives: Cutaneous wounds are seriously painful and their antibiotic treatments are expensive with adverse effects. Nowadays medicinal herbs are widely used because of lower cost and side effects. Calendula officinalis, Aloe arborescens and Ricinus communis herbs have antimicrobial and healing effects. The aim of the present study was comparing the effect of these three herbal medicines on cutaneous wound healing process in male rat.
Materials & Methods: In this experimental study, 32 male adult rats were randomly divided into 4 equal groups of 8, including one control group (wound group and topical use of eucerine in the injured area) and three groups treated with Calendula officinalis, Aloe arborescens and Ricinus communis (topical use of 2cc extract 10%). Wounds with equal thickness were made on the rats’ back sides and their diameters were measured in the treatment period (21 days) and tissue samples were finally prepared. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Results: Diameter measures of the wounds on the 5th day showed that the average of the wounds’ diameters in the Calendula officinalis and Ricinus communis treated groups were significantly lower than the control group (p<0.05). At the end of the experiment, the best improvement on cutaneous wound healing  belonged to the group treated with Ricinus communis so that there was a significant difference between this group and the control group (p<0.05).
Conclusion: Ricinus communis medicinal herb has more improving effect on cutaneous wound healing in rat.
Key words: Calendula officinalis, Aloe arborescens, Ricinus communis, Wound healing
 
Funding: This research was funded by University of Zabol and Tehran University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of University of Zabol approved the study (UOZ.ECRA.2017.2316905).
 
How to cite this article: Davari SA, Hajinezhad MR, Samadi K, Eftekhari S. The Effect of Calendula, Aloe and Caster on Cutaneous Wounds Healing Process in Mature Male Rat. Univ Med Sci 2018; 17(2): 93-104. [Farsi]
 
Study of Hematological, Biochemical and Histopathological Changes Due to Long-Term Administration of Ketamine in Rat
M. Hashemnia, M. Javedani, Z. Nikoosafat, S. Abdoli Jamoor,
Volume 17, Issue 7 (11-2018)
Abstract
Background and Objectives: Nowadays, the use of ketamine has spread as a recreational drug. Therefore, this study was performed to evaluate the effects of long-term administration of ketamine on some hematological and biochemical parameters and tissue changes in rats.
Material and Methods: in this experimental study, forty male Sprague-Dawley rats were randomly allocated into two control (sham) (n=20) and experimental (n=20) groups. The animals in the sham group received saline solution and the experimental group received ketamine (15 mg/kg) as intraperitoneal injection. At the days of 0, 20, 40, and 60 post injection, five rats from each group were euthanized and anatomized. Some biochemical parameters of the serum were measured and the livers and kidneys tissues underwent microscopic examination. The ANOVA,Tukey post hoc test, and non-parametric Kruskal-Wallis test were used for data analysis.
Results: The results indicated that ketamine caused an increase in the number of White Blood Cells and liver enzymes levels, the creatinine, urea, cortisol, and glucose concentrations and a decrease in the total protein and globulin in serum of ketamine group as compared to the control group. In examining the liver, vacuolar degeneration, congestion and dilation of sinusoids, infiltration of mononuclear cells around the portal area, and parenchymal necrosis were observed, and tubular epithelial cell degeneration and necrosis, tubular and glomerular atrophy, and infiltration of mononuclear cells were observed in the kidney.
Conclusion: The results indicated that ketamine causes kidney and liver tissues damages in rats. However more comprehensive studies are needed to determine the mechanism of ketamine’s action in long-term use.
Key words: Ketamine, Hematological changes, biochemical changes, Histopathology, Rat
 
Funding: There was no fund for this study.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Razi University of Kermanshah approved the study (397-2-02).
 
How to cite this article: Hashemnia M, Javedani M, Nikoosafat Z, Abdoli Jamoor S. Study of Hematological, Biochemical and Histopathological Changes Due to Long-Term Administration of Ketamine in Rat[a1] . [12] J Rafsanjan Univ Med Sci 2018; 17 (7): 639-56. [Farsi]
 [a1]ذکر وابستگی سازمانی هر نویسنده به زبان انگلیسی ضروری است. هم­چنین در انتهای وابستگی سازمانی هر نویسنده به زبان انگلیسی، ذکر ORCID برای هر نویسنده ضروری است.
 [12]افیلییشن طبق چکیده فارسی ترجمه شد و همانطور که ذکر شده نویسنده محترم مسئول مشخص شده، در بخش فارسی و انگلیسی با یکدیگر هم­خوانی ندارند.
 

Association of rs6543115 Single Nucleotide Polymorphism in the IL1RL1 Gene Promoter with Increased Risk of Colon Cancer: A Descriptive Study
T. Dehbozorgi, Dr M. Moghanibashi, Dr P. Mohamadynejad,
Volume 20, Issue 4 (7-2021)
Abstract
Background and Objectives: One of the genes that has been proven to play a role in colorectal cancer is IL1RL1 (Interleukin 1 receptor like 1). This gene encodes a receptor for interleukin 33 that has been shown with its ligand, to be involved in colorectal cancer. Several single nucleotide polymorphisms are involved in regulating the expression of IL1RL1 gene, so that this study investigated the association between genotypes of rs6543115 polymorphism in the promoter of IL1RL1 gene and colon cancer.
Materials and Methods: In this descriptive study, genomic DNA was extracted from blood samples of 100 patients with colon cancer and 100 healthy individuals. The region containing the desired polymorphic site was amplified using appropriate primers and PCR technique, and then was sequenced, and all samples were genotyped. Finally, logistic regression analysis and chi-square test were used to assess the association between rs6543115 genotypes in IL1RL1 gene and colon cancer.
Results: The results showed that genotype GC (OR= 0.273, 95%CI: 0.090-0.827, p= 0.022) and CC (OR= 0.086, 95%CI: 0.032-0.232, p<0.001) as a risk factor in comparison to genotype GG, significantly decreased the susceptibility of colon cancer. Also, the results showed that the allele C, in comparison to allele G, significantly reduced the risk of colon cancer (OR= 0.119, 95%CI: 0.066-0.232, p< 0.001).
Conclusion: The rs6543115 polymorphism in the IL1RL1 gene appears to be associated with the risk of colon cancer.
Key words: Colon cancer, IL1RL1 gene, rs6543115 polymorphism
 
Funding: This study was funded by Islamic Azad University, Kazerun Branch.
Conflict of interest: None declared
Ethical approval: The Ethics Committee of the Islamic Azad University, Kazerun Branch, approved the study (IR.IAU.KAU.REC.1398.013).
 
How to cite this article: Dehbozorgi T, Moghanibashi M, Mohamadynejad P. Association of rs6543115 Single Nucleotide Polymorphism in the IL1RL1 Gene Promoter with Increased Risk of Colon Cancer: A Descriptive Study. J Rafsanjan Univ Med Sci 2021; 20 (4): 405-14. [Farsi]

Survey of Compliance with Ethical Considerations in Laboratory Animal Research Projects in Hormozgan University of Medical Sciences during 2018-2021: A Short Report
Saber Yazdani Damavandi, Zeinab Esmaeilzadeh, Nasrin Davari Dolatabadi,
Volume 22, Issue 3 (6-2023)
Abstract

Background and Objectives: The use of criteria and ethical frameworks is critical in all research projects.  Therefore, the purpose of this study was to determine the ethical considerations in laboratory animal research projects.
Materials and Methods: In this descriptive cross-sectional study, 40 research proposals related to laboratory animals in Hormozgan University of Medical Sciences were collected during the years 2018-2021 and then, their compliance with ethical considerations was examined using the monitoring form of laboratory animals. The results were reported as numbers and percentages.
Results: The results indicated that animal welfare was considered in 38 proposals (95%). In addition, animal safety and health considerations, non-surgical and non-invasive interventions, anesthesia and analgesia measures, and surgical and invasive interventions were mentioned in 39 proposals (97.5%).
Conclusion: It seems that more attention to ethical principles is needed in working with animals in research projects.
Key words: Laboratory animals, Animal model, Ethics, Animal welfare

Funding: This study was funded by Hormozgan Faculty of Para-medicine.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Hormozgan University of Medical Sciences approved the study (IR.HUMS.REC.1400.382).

How to cite this article: Yazdani Damavandi Saber, Esmaeilzade Zeinab, Davari Dolatabadi Nasrin. Survey of Compliance with Ethical Considerations in Laboratory Animal Research Projects in Hormozgan University of Medical Sciences During 2018-2021: A Short Report. J Rafsanjan Univ Med Sci 2023; 22 (3): 293-300. [Farsi]



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