Background and Objective: Recently, it is considered the role of hypoglycemic agents specially
medicinal plants derivatives in treatment of diabetes mellitus. Aloe Vera
contains phenolic compounds and flavonoids with antioxidant properties that reduces
the complications of the disease.The aim of study was to investigate the
effects of hydroalcoholic extract of Aloe Vera in serum levels of glucose and
insulin and key enzymes in metabolic pathways of glycolysis and gluconeogenesis
in streptozotocin-induced diabetic Wistar rats.
Materials and Methods: A total of 50 rats (initial weight of 220 ±10
g) were divided into five groups. Two months after treatment with Aloe Vera
extract, the rats were sacrificed and blood glucose and serum insulin levels
were measured. It was also isolated the liver then, extracted mRNA from it,
expression levels of Glucokinase and Phosphoenol pyruvate carboxy kinase
enzymes were measured by Real Time PCR.
Results: The results showed that the extract of Aloe Vera at doses of 100,
200 and 400 mg/kg increase insulin secretion (p<0.05), hypoglycemia
(p<0.001), increasing expression of Glucokinase enzyme (p<0.05) and reducing
expression of the Phosphoenol pyruvate
carboxy kinase enzyme (p<0.05) in diabetic rats.
Conclusion: Not only dose Aloe Vera hydroalcoholic
extract influence on insulin secretion, it also affects on key enzymes gene
expression of Glycolysis and Gluconeogenesis pathways. Hence, this extract can
be effective for improvement of diabetic complications.
Key words: Glycolysis and Gluconeogenesis pathways key enzymes, Streptozotocin, Aloe Vera, Rat
Funding: This research was
funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University
of Medical Sciences approved the study.
How to cite this article: Chahardoli M, Mahmoodi M, Hajizadeh MR, Khoramdel Azad H,
Khoshdel AR, Mirzae MR. Effect of Aloe Vera Hydroalcoholic Extract on Blood
Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis
and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats. J RafsanjanUniv Med Sci 2014 13(8):
668-82 [Farsi]
