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Showing 6 results for Rezazadeh Zarandi
Antagonistic Effects of Lactobacillus against Helicobacter pylori in a Gel Stabilized Glucose - Gradient System
H. Abdollahi, E. Rezazadeh Zarandi,
Volume 5, Issue 2 (6-2006)
Abstract

  Antagonistic Effects of Lactobacillus against Helicobacter pylori in a Gel Stabilized Glucose - Gradient System

 

  H. Abdollahi PhD [1] , E. Rezazadeh Zarandi MSc [2]

 

  Recived: 30/01/2006 Revised: 27/06/2006 Accepted: 16/07/2006

 

  Background and Objective: Helicobacter pylori is a gram-negative bacterium inhabits the gastric mucosa and may remains there for a long period. It causes gastritis and ulcer. Recently, antibiotics are widely used to treat the infections caused by Helicobacter pylori but other means of treatment such as Probiotic (using live organisms such as Lactobacilli) can be considered. Therefore in this study antagonistic effects of two species of Lactobacillus against Helicobacter pylori under the laboratory condition are investigated.

  Materials and Methods: In this study a gel stabilized system was used. This system consisted of two phases solid layer (10 ml peptone yeast extract salt solution plus 1.5% agar and 2% glucose) at the bottom and a semi layer (10 ml PYES plus 0.75% agar and 1.5 × 107 washed bacterial cell /ml) at the top in 30 ml screw test tubes. Lactobacilli and Helicobacter pylori were used as pure and mixed in the upper phase of the above system. After incubation at 37 ºC for different time intervals , samples were withdrawn from the semisolid layer using sterilized steel corkscrew and sectioned. The bacterial population, optical density, pH and glucose concentration of the medium were measured at the various depths.

  Results: All bacteria had a condensed band of growth in the special places of semisolid. Growth appearance (growth band) viable count, optical density, pH in the mixed culture of Lactobacillus plantarum and Helicobacter pylori were like the pure culture of Lactobacillus plantarum. However, mixed culture of Lactobacillus casei and Helicobacter pylori was similar to that of pure culture of Helicobacter pylori.

  Conclusion: Regarding the results of this study Lactobacillus plantarum inhibited the growth of Helicobacter pylori in the mixed culture. This may be due to possible production of organic acids like lactic acid and bacteriocins, but Lactobacillus casei could not inhibit the Helicobacter pylori’s growth. Therefore, Lactobacillus plantarum has antagonistic effect against Helicobacter pylori under in vitro condition and it can be a good choice for the probiotic purposes against Helicobacter pylori infections for in vivo investigations.

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  Key words: Helicobacter pylori, Lactobacillus, Probiotic, Gastric Ulcer, Duodenal Ulcer



  [1] - Associate Prof. Dept. of Microbiology, University of Medical Sciences, Kerman

  (Corresponding Author) Tel: ( 0341) 3221665, Fax: (0341) 3221671, E-Mail: hamid-abdollahi@yahoo.com

  [2] - Academic Member Dept. of Microbiology, University of Medical Sciences, Rafsanjan


Evaluation of Occult Hepatits B Virus Infection in Thalassemic Patients Infected by HCV in Kerman Province of Iran
M. Kazemi Arababadi, G. Hassanshahi, M. Rezaeian, E. Rezazadeh Zarandi, R. Vazirinejad,
Volume 8, Issue 4 (12-2009)
Abstract

  Evaluation of Occult Hepatits B Virus Infection in Thalassemic Patients Infected by HCV in Kerman Province of Iran

 

  M. Kazemi Arababadi [1] , Gh. Hassanshahi [2] , M. Rezaeian [3] , E. Rezazadeh Zarandi [4] , R. Vazirinejad [5]

 �

  Received: 03/01/09 Sent for Revision: 08/03/09 Received Revised Manuscript: 31/10/09 Accepted: 20/12/09

 �

  Background and Objectives: Thalassemic patients are at risk of blood transmitted infectious diseases such as hepatitis B and C, due to continued receiving of blood and its components . The aim of this study was to investigate the prevalence of occult Hepatits B Virus infection (OBI) and HBV markers in thalassemic patients with Hepatitis C infection.

  Materials and Methods : In this cross-sectional study, 60 thalassemic patients were examined for HCV infection by Reverse Transcription-PCR (RT-PCR) during Apr-Sep 2007 in the Kerman province of Iran. Hepatitis B virus infection was evaluated in the HCV positive thalassemic patients by PCR. Anti-HBc, anti-HBs and HBsAg were detected using ELISA.

  Results : Results indicated that although 27 (45%) of the cases, out of 60, were infected by HCV, but HBV-DNA was not detected in the HCV infected patients. The findings also showed that 9 (33%) out of 27 HCV-RNA positive patients were anti-HBc positive and 11 (40.7%) out of 27 patients were positive for anti-HBs.

  Conclusion: Results of this study indicated that the prevalence of OBI was very low in thalassemic patients (in contrast to HCV infection). However, this value is probably due to the inhibitory effects of HCV on host cell translation system which affects HBV protein translation.

  Key words: H epatitis B , H epatitis C, Hepatitis C Virus , Hepatitis B Virus , O ccult Hepatitis B Infection, Thalassemia

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  Funding: This research was funded by Rafsanjan University of Medical Sciences.

  Conflict of interest: None declared.

  Ethical approval: The Ethical Committee of Rafsanjan University of Medical Sciences approved the study.


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  [1] - Instructor , Dept. of Microbiology and Immunology, Faculty of Medicine, University of Medical Sciences, Rafsanjan, Iran

  (Corresponding Author) Tel: (0391)5234003, Fax: (0391) 5225209, E-mail: kazemi24@yahoo.com

  [2] - Assistant Prof., Dept. of Hematology, Faculty of Medicine, University of Medical Sciences, Rafsanjan, Iran

  [3] - Associate Prof., Dept. of Social Medicine, Faculty of Medicine , University of Medical Sciences, Rafsanjan, Iran

  [4] - Instructor, Dept. of Microbiology, Faculty of Medicine, University of Medical Sciences, Rafsanjan, Iran

  [5] - Associate Prof., Dept. of Social Medicine, Faculty of Medicine, University of Medical Sciences, Rafsanjan, Iran


Diagnosis of Bacterial Infections; Traditional and Molecular Methods: A Narrative Study
A. Darekordi, E. Rezazadeh Zarandi, S. Assar, O. Rezahosseini, Sh. Assar,
Volume 17, Issue 9 (12-2018)
Abstract
Background and Objectives: Traditionally, diagnosis of the agents of infectious diseases is based on conventional or phenotypic methods. Due to the lack of growth of some infectious agents, these methods are very difficult or impossible for diagnosis of some bacteria. The advent of molecular techniques has more or less influences on the detection of microbial infections. This article addresses the advantages and disadvantages of diagnostic molecular and conventional methods in detecting pathogenic microorganisms. Traditional diagnostic methods such as phenotypic, chemical and serologic characteristics are still used for detection of infectious diseases. In addition to diagnosing the bacterial agents, varient molecular methods may also detect virulence factors and antimicrobial resistance genes at a higher rate, sensitivity and accuracy. It seems that the advanced molecular methods can reduce diagnosis time, mortality rate and costs of laboratory diagnosis, and shorten the duration of hospitalization.
Key words: Diagnosis, Infectious diseases, Traditional, Molecular, Method
 
Funding: This study did not have any funds.
Ethical approval: None declared.
Conflict of interest: None declared.
 
 
How to cite this article: Darekordi A, Rezazadeh Zarandi E, Assar S, Rezahosseini R, Assar Sh. Diagnosis of Bacterial Infections; Traditional and Molecular Methods: A Narrative Study. J Rafsanjan Univ Med Sci 2018; 17 (9): 865-80. [Farsi]
 


Comparison of Antimicrobial Effects of Matrica Herbal Mouthwash and 0.2% Chlorhexidine on the Growth of Streptococcus Mutans, Actinomyces Viscosus, and Enterobacter Cloacae: A Short Report
S. Salari Sedigh, M. Sadeghi, M. Beikizadeh, E. Rezazadeh Zarandi, Sh. Assar,
Volume 18, Issue 3 (6-2019)
Abstract
Background and Objectives: Mouthwashes are commonly used to reduce the harmful effects of oral bacteria. The purpose of the present study was to compare the antibacterial effects of Matrica and Chlorhexidine 0.2% mouthwashes on common microorganisms in periodontal diseases.
Materials and Methods: This is a laboratory study in which disc diffusion method was used. The diameter of the inhibition zone caused by the antimicrobial effect of Matrica and Chlorhexidine 0.2% was measured in millimeter. Data were analyzed using independent t-test and one-way ANOVA.
Results: The highest mean diameter of the inhibition zone caused by Matrica was 14.85±0.42 and in case of Chlorhexidine 0.2%, 25.54±0.49. Chlorhexidine showed more inhibitory effect compared to Matrica (p˂ 0.001).
Conclusion: The results of this study showed that chlorhexidine compared to Matrica has a more antibacterial effect on bacteria that are effective in periodontal diseases. Matrica mouthwash has a more inhibitory effect on the Actinomyces viscosus compared with the other two bacteria.
Key words: Anti-infective agents, Chlorhexidine, Matrica, Streptococcus mutans, Actinomyces viscosus,  Enterobacter cloacae
 
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: None declared.
How to cite this article: Salari Sedigh S, Sadeghi M, Beikizadeh M, Rezazadeh Zarandi E, Assar Sh. Comparison of Antimicrobial Effects of Matrica Herbal Mouthwash and 0.2% Chlorhexidine on the Growth of Streptococcus Mutans, Actinomyces Viscosus, and Enterobacter Cloacae: A Short Report. J Rafsanjan Univ Med Sci 2019; 18 (3): 305-12. [Farsi]
 
The Frequency of Antibiotic Resistance of Clinical Isolates of Clostridioides difficile to Commonly Used Antibiotics in Hospitalized Patients of Educational Hospitals of Kerman City During 2018-2020
Mohammadsaeed Shojaei, Farokh Rokhbakhsh-Zamin, Ebrahim Rezazadeh Zarandi, Farhad Sarafzadeh, Sayed Mohammad Reza Khoshroo,
Volume 21, Issue 9 (12-2022)
Abstract
Received: 18/09/22 Sent for Revision: 23/10/22 Received Revised Manuscript: 13/12/22 Accepted: 14/12/22
Background and Objectives: Clostridioides difficile (C. difficile) is one of the main causes of antibiotic-associated diarrhea. It is important to find out the pattern of its antimicrobial susceptibility for reducing prevalence and also treating C. difficile infection (CDI). This study aims to investigate the antimicrobial resistance of C. difficile to commonly used antibiotics, especially vancomycin and metronidazole, as the most effective antibiotics against the bacterium.
Materials and Methods: In this descriptive study, 417 diarrheal stool samples were taken from hospitalized patients of educational hospitals of Kerman City from 2018 to 2020. The samples were cultured on cycloserine-cefoxitin fructose agar (CCFA), and suspected C. difficile colonies were isolated. Identification of the cdd-3 gene for definitive diagnosis of C. difficile was performed. Antibiotic resistance test was conducted by the disk-diffusion method using vancomycin, metronidazole, rifampin, amoxicillin-clavulanic acid, erythromycin, imipenem, ciprofloxacin, and clindamycin disks. The results were reported as numbers and percentages.
Results: A total of 68 (16.3%) isolates of C. difficile were taken from the samples. Most C. difficile strains were susceptible to vancomycin and metronidazole, while the highest rate of resistance was related to ciprofloxacin and clindamycin. The prevalence of multi-drug resistant (MDR) strains was 77.9%.
Conclusion: The results of this study showed that vancomycin is still the best antibiotic for treating CDI. Also, the frequency of the isolates resistant to CDI-inducing antibiotics (erythromycin, clindamycin, ciprofloxacin) and MDR isolates was high. Therefore, the spread of resistant strains of C. difficile can be prevented by appropriate antibiotic prescription.
Key words: Clostridioides difficile, Antibiotic resistance, Multi-drug resistant (MDR), Kerman

Funding: This study did not have any funds.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Kerman University of Medical Sciences approved this study (IR.KMU.REC.1398.082).
How to cite this article: Shojaei MohammadSaeed, Rokhbakhsh-Zamin Farokh, Rezazadeh Zarandi Ebrahim, Sarafzadeh Farhad, Khoshroo Sayed Mohammad Reza. The Frequency of Antibiotic Resistance of Clinical Isolates of Clostridioides difficile to Commonly Used Antibiotics in Hospitalized Patients of Educational Hospitals of Kerman City During 2018-2020. J Rafsanjan Univ Med Sci 2022; 21 (9): 955-70. [Farsi]
 
Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report
Abdolreza Babaee, Ebrahim Rezazadeh Zarandi, Ahmad Shabanizadeh, Zahra Taghipour, Samereh Dehghani-Soltani, Mahnaz Ramezani, Amirhesam Khodaei, Mohammad Mohsen Taghavi,
Volume 23, Issue 6 (10-2024)
Abstract 
Background and Objectives: The aim of this study was to investigate the effect of sheep lymph nodes and antibiotics on the bacterium that causes acne and the bacteria that usually exist with it.
Materials and Methods: In this laboratory study, 24 patients with acne (18 to 45 years old) were selected to isolate the bacteria. Also, 24 clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis were isolated from the wound samples of patients. After culturing the bacteria, their sensitivity to common antibiotics was evaluated. Data were reported as percentage.
Results: The results showed that Cutibacterium acnes and Staphylococcus aureus were 100% sensitive to vancomycin. Also, Cutibacterium acnes showed sensitivity in tetracycline and imipenem groups in 95% of cases and in cotrimoxazole and piperacillin/tazobactam groups in 90% of cases. In addition, the highest sensitivity of Staphylococcus epidermis to piperacillin/tazobactam (62.5%) and the highest drug resistance (91.67%) were related to azithromycin and erythromycin groups.
Conclusion: Due to the increase in drug resistance, it is suggested to use antibiotics with the least drug resistance such as vancomycin and imipenem in the treatment of acne.
Keywords: Lymph node, Antibiotic, Acne, Staphylococcus aureus, Cutibacterium acnes

Funding: This study was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical considerations: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1399.103).

Authors’ contributions:
- Conceptualization: Mohammad Mohsen Taghavi, Ebrahim Rezazadeh Zarandi
- Methodology: Mohammad Mohsen Taghavi, Ahmad Shabanizadeh
- Data collection: Mahnaz Ramezani, Amir Hesam Khodaei
- Formal analysis: Abdolreza Babaee, Samereh Dehghani-Soltani
- Supervision: Mohammad Mohsen Taghavi, Zahra Taghipour
- Project administration: Mohammad Mohsen Taghavi
- Writing - original draft: Abdolreza Babaee, Ebrahim Rezazadeh Zarandi
- Writing - review & editing: Mohammad Mohsen Taghavi, Abdolreza Babaee
 
Citation: Babaee A, Rezazadeh Zarandi E, Shabanizadeh E, Taghipour Z, Dehghani-Soltani S, Ramezani M, Khodaei AH, Taghavi MM. Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report. J Rafsanjan Univ Med Sci 2024; 23 (6): 568-75. [Farsi]

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