Journal of Rafsanjan University of Medical Sciences

  Background and Objectives : RUNX2 is the most specific transcription factor in osteoblastic differentiation of MSCs. In this research, RUNX2 expression was quantified in MSCs differentiated by osteogenic differentiation medium (ODM) and zoledronic acid (ZA).

  Materials and Methods: In this experimental study, hMSCs were treated by osteogenic differentiation medium and ZA. RNA extraction was carried out from both osteoblastic differentiated cells in the first, second and third weeks of differentiation, and also from undifferentiated MSCs. RUNX2 expression was quantified by quantitative Real Time-PCR.

  Results: Gene expression of RUNX2 in the first, second and third weeks of osteogenic differentiation by ODM compared to undifferentiated MSCs showed 1.76±0.09-fold, 3.54±0.25-fold and 3.40±0.17-fold increase in expression, respectively. Zoledronic acid increased the expression of RUNX2 2.91±0.13-fold, 3.25±0.3-fold and 3.36±0.23-fold at the same time points, respectively. Comparison of RUNX2 expression by ODM (1.76±0.09-fold) and ZA (2.91±0.13-fold) in the first week of differentiation showed statistical differences (P<0.05). Whereas, RUNX2 expression by both ODM and ZA in the second and third weeks of differentiation were approximately equal.

  Conclusion: RUNX2 expression was increased in osteoblastic differentiation by both ODM and ZA. However, it seems that ZA can cause more expression of RUNX2 in the first week of osteoblastic differentiation.

  Key words: Mesenchymal Stem Cells, Differentiation Medium, Zoledronic Acid, RUNX2 Transcription Factor

  Funding: This research was funded by Tarbiat Modares University.

  Conflict of interest: None declared.

  Ethical approved: The Ethics Committee of Tarbiat Modares University approved the study .

  How to cite this article : Farshdousti Hagh M , Noruzinia M, Mortazavi Y, Soleimani M, Kaviani S, Mahmoodinia Maymand M . Comparison of Quantitative Expression of Runx2 in Mesenchymal Stem Cells (Mscs) Differentiated by Osteoblastic Differentiation Medium and Zoledronic Acid . J Rafsanjan Univ Med Scie 2012 11(4): 377-90. [Farsi]

Background and Objective: Various diseases and injuries can lead to loss cartilage tissue. Cartilage tissue engineering based on the use of stem cells which has provided a promising opportunity to repair damaged tissue. Recently, adipose-derived mesenchymal stem cells (ADSCs) have captured considerable scientific and clinic interest because of their easy access, rapid expansion in vitro and ability to differentiate into diverse cell lines. The aim of this study was to introduce the pellet culture system as an in vitro model for cartilage engineering approaches.

Materials and Methods: This experimental study was performed in Stem Cell laboratory, The Academic Center for Education, Culture and Research, Qom in 2013. In this study, ADSCs were isolated and expanded from human adipose tissue. The cells were centrifuged and the chondrogenic differentiation was performed in pellet culture system. Viability potential and chondrogenic genes expression were evaluated by MTT assay and Real-time PCR analysis, respectively. In this study, data statistical analysis was carried out by independent T-test.

Results: In this study, our obtained results using MTT assay and Real-time PCR analysis demonstrated cell survival ability and proliferation and also the expression of chondrogenic-specific genes at pellet in comparison with control group.

Conclusion: It is suggested that this method can be used for induction of chondrogenic.

Key words: Adipose-derived mesenchymal stem cells, Pellet culture system, Differentiate into chondrocyte

Funding: This research was funded by The Academic Center for Education, Culture and Research, qom-Iran.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of The Academic Center for Education, Culture and Research, qom-Iran approved the study.

How to cite this article: Tabatabaei Qomi R, Sheykhhasan M, Kalhor N, Ghiasi M. Introduction of Pellet Culture System of Human Adipose-Derived Mesenchymal Stem Cells as an in vitro Model for Cartilage Engineering Approaches. J RafsanjanUniv Med Sci 2015 14(4): 269-82. [Farsi]