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Showing 3 results for Pentylenetetrazol
Effects of the Aqueous and Alcoholic Extracts of Anethum Graveolens L (dill) on Pentylenetetrazol-induced Seizures in Male Mice
M. Mohammadzadeh, A. Pajhhan, S.j. Mirnajafizadeh, M.h. Rakhshani,
Volume 11, Issue 1 (4-2012)
Abstract

  Effects of the Aqueous and Alcoholic Extracts of Anethum Graveolens L (dill) on Pentylenetetrazol-induced Seizures in Male Mice

  

  M. Mohammadzadeh [1] , A. Pajhhan1, S.J. Mirnajafizadeh [2] , M.H. Rakhshani [3]

  

  Received: 13/10/2010 Sent for Revision: 13/11/2010 Received Revised Manuscript: 05/04/2011 Accepted: 26/04/2011

  

  Background and Objectives : In this study, the effect of Anethum graveolens L. extract on pentylenetetrazol- (PTZ) induced seizures was investigated.

  Materials and Methods: In this experimental study, 110 male mice were included. Firstly, the maximum non-lethal doses for aqueous and alcoholic extract of Anethum graveolens L. were determined. Three doses of aqueous (250, 400, and 500 mg/kg) and four doses of alcoholic extracts (250, 1000, 2500, and 3500 mg/kg) were intraperitoneally injected 30 minutes before PTZ injection. All doses were lower than the non-lethal dose Seizure stage 1 latency (S1L), seizure stage 5 latency (S5L) and dead time latency (DTL) were recorded after PTZ injection in both control and treatment groups. One-way and two way ANOVA were applied for comparing the results of this study.

  Results: Aqueous extract of Anethum graveolens L . at 500 mg/kg increased S1L, S5L and DTL significantly (p<0.01, p<0.05 and p<0.01 respectively). Concentration of 400 mg/kg was able to markedly increase only S1L and DTL (p<0.05 and p<0.01 respectively) and Concentration or 250 mg/kg had no any effects.

  Injection of the alcoholic extract at concentrations of 1000, 2500 and 3500 mg increased S1L, S5L and DTL significantly. However, concentration of 250 mg/kg significantly increased only S1L and DTL (p<0.05 and p<0.01 respectively). There was no significant between aqueous and alcoholic extract except in DTL.

  Conclusion: The results indicated that the aqueous and alcoholic extractions of Anethum graveolens L. possibly have protective effects on nervous system and also decrease seizure induced by PTZ.

  Key words: Seizure, Epilepsy, Pentylenetetrazol, Anethum graveolens, Mice

  

  Funding: This research was funded by Sabzevar University of Medical Sciences.

  Conflict of interest: None declared.

  Ethical approval: The Ethics Committee of Sabzevar University of Medical Sciences approved the study.

  

  How to cite this article : Mohammadzadeh M, Pajhhan A, Mirnajafizadeh S.J, Rakhshani M.H. Effects of the Aqueous and Alcoholic Extracts of Anethum Graveolens L (dill) on Pentylenetetrazol-induced Seizures in Male Mice. J Rafsanjan Univ Med scie 2012 11(1): 47-56. [Farsi]

 


  

  [1] - Assistant Prof., Dept. of Physiology, Sabzevar University of Medical Sciences, Sabzevar, Iran

  (Corresponding Author) (0571) 4446070, Fax:(0571) 4445648, E-mail: mohamad1353@gmail.com

  [2] - Prof., Dept. of Physiology, Tarbiat Modarres University, Tehranl, Iran

  [3] - Academic Member, Dept. of Statistics, Sabzevar University of Medical Sciences, Sabzevar, Iran


Effects of Intracerebroventricular Administration of Salubrinal-A Protein Synthesis Inhibitor-on Epileptic Seizures Induced by Pentylenetetrazol in Male Rats
M. Izadi, M.e. Rezvani, M. Esmaeilidehaj,
Volume 13, Issue 3 (10-2014)
Abstract

Background and Objective: Salubrinal- a protein synthesis inhibitor- plays a crucial role in sleep and neuronal inhibition through the GABA activation. According to these evidences, the aim of the present study was to determine the effect of salubrinal on epileptic seizures induced by pentylenetetrazol (PTZ).

Materials and Methods: In this expremental study under stereotaxical surgery, three monopolar electrodes were fixed on the skull bone of animals and a cannula was implanted in the right cerebral ventricle according to Paxinos coordinates. After 7 days of recovery, each animal received intracerebroventricular (ICV) vehicle, diazepam (0.2 mg/rat) or salubrinal (25 or 50 µg/rat) 10 minutes before Pentylenetetrazol injection (subcutaneous, 60mg/kg). Electrophysiological and behavioral parameters of induced seizures were recorded during 60 minutes.

Results : Salubrinal (25 and 50 µg/rat) significantly retarded the onset of generalized tonic-clonic seizures when compared to vehicle treatment (p<0.05). Epileptic discharge duration and ictal discharge duration were significantly shortened in salubrinal treated rats (p<0.05). Additionally, the rats treated with salubrinal (25 and 50 µg/rat) had lower seizure stage scores, as compared to vehicle treated rats (p<0.05).

Conclusion : The findings of this study revealed that shortly after ICV. administration of salubrinal, epileptic seizures can be significantly decreased. Also, the findings suggest that protein synthesis is an essential process during development of epileptic seizures.

Key words : Seizure, Salubrinal, Protein synthesis inhibitor, Rat, Pentylenetetrazol

 

Funding: This research was funded by Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Conflict of interest: None declared.

Ethical approval: The Research Committee of Shahid Sadoughi University of Medical Sciences approved the

study.

 

How to cite this article: Izadi M, Rezvani ME, Esmailidehaj M. Effects of Intracerebroventricular Administration of Salubrinal-A Protein Synthesis Inhibitor-on Epileptic Seizures Induced by Pentylenetetrazol in Male Rats. J Rafsanjan Univ Med Sci 2014 13(3): 281-92. [Farsi]


The Effect of Maternal Prenatal Stress on Frontal Lobe Evolution and Evaluation of Seizure Threshold in NMRI Mice Offsprings
F. Hakimi, Gh. Kaka, M. Sadoughi,
Volume 16, Issue 9 (1-2018)
Abstract

Background and Objectives: Maternal prenatal stress while increasing the glucocorticoid level in the embryo, has detrimenal effects on the neural structure. This study examined  the effect of maternal stress on the change of the frontal lobe in mice during pregnancy and also the seizure threshold in their offsprings.
Materials and Methods: In this experimental study, 30 pregnant female mice were divided into two equal groups: the non-stress group and stress group. The stress group experienced one hour immobilization stress for 14 days. After child birth, offsprings were divided into three groups (n=4): The control group, mothers received no immobilization stress and their offsprings also received no PTZ;the Sham  group, mothers received no immobilization stress but their offsprings received PTZ; and the experimental group, mothers received immobilization stress and their offsprings also received PTZ. To study the frontal lobe of mouse embryo, the brain of mice offisprings were removed and fixed.The sections (5 micron) were prepared and stained byH&E technique. Histological studies were performed using the Histolab and Motic softwares.
Results: The results showed a significant increase in seizure threshold in the offsprings whose mothers were under immobilization stress compared with the offsprings whose mothers received no stress (p<0.001). A significant reduction in the thickness of layers was observed in the experimental group compared with the control and sham groups (p<0.05). The number of neural and glial cells and the number of blood vessels in the experimental group  significantly decreased compared with the control and sham groups (p<0.05).
Conclusion: Imobilization stress during pregnancy can cause an increase in the seizure threshold in mice offisprings and a disorder in the development and structure of  their frontal lobe.
Keywords: Frontal lobe, Seizure, Stress, Pentylenetetrazole, Histomorphometry.
 
Funding: None declared.
Conflict of interest: The authors have no conflicts of interest.
Ethical approval:Approval for this experimental study was obtained from the Institutional Review Board, and all experiments were carried out in accordance with the Guidelines of Animal Care and Use of the Ethics Committee of Baqiyatallah University of Medical Sciences
 
How to cite this article: Hakimi F , Kaka Gh, Sadoughi M. The Effect of Maternal Prenatal Stress on Frontal Lobe Evolution and Evaluation of Seizure Threshold in NMRI Mice Offsprings. J Rafsanjan Univ Med Sci 2017; 16(9): 845-56. [Farsi]
 


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