Showing 2 results for Selenium Nanoparticles
M. Shakibaie, M. Jafari, A. Ameri, H.r. Rahimi, H. Forootanfar,
Volume 17, Issue 7 (11-2018)
Abstract
Background and Objectives: Due to the unique physicochemical properties of selenium nanoparticles (Se NPs), identification of microbial strains capable to biosynthesize Se NPs has recently attracted attention. The current study aimed at introducing Se NPs producing actinomycete strain, characterizing Se NPs as well as evaluating its cytotoxic effect against breast cancer cell line (MCF-7).
Materials and Methods: In the present laboratory investigation, first, the Se NPs producing strain was isolated from soil samples. The selected isolate was then identified using morphological and biochemical examinations as well as 16S rDNA sequencing protocol. The UV-visible spectrum, particle-size distribution (PSD) pattern, Fourier-transform infrared (FTIR), and energy-dispersive X-ray (EDX) profiles of the nanostructures as well as transmission electron microscope (TEM) image of Se NPs were determined. In order to evaluate the cytotoxicity of the Se NPs, the MTT (Methylthiazolyldiphenyl-tetrazolium bromide) based colorimetric protocol was applied where the viability percent was firstly determined and then the related IC50 (Half inhibitory concentration) was calculated.
Results: The selected bacterial isolate was identified as Streptomyces lavendulae FSHJ9. TEM micrographs of the biogenic Se NPs exhibited spherical nanostructures with the size range of 28–123 nm. The FTIR pattern showed no functional group present on the surface of Se NPs. The obtained results of cytotoxicity revealed that IC50 of Se NPs (77.1±42.23 µg/mL) was more than IC50 of sodium selenite (3.0±41.53 µg/mL).
Conclusion: The results of the present study showed that Streptomyces lavendulae FSHJ9 was able to produce Se NPs. The produced biogenic Se NPs, after performing complementary studies, might be applied as supplement in human food and animal feeding.
Key words: Selenium nanoparticles, Biosynthesis, Streptomyces, Cytotoxicity, MCF-7 cell line
Funding: This study was funded by Kerman University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Kerman University of Medical Sciences approved the study (930160).
How to cite this article: Shakibaie M, Jafari M, Ameri A, Rahimi H.R, Forootanfar H. Biosynthesis and Physicochemical Characterization, and Cytotoxic Evaluation of Selenium Nanoparticles Produced by Streptomyces Lavendulae FSHJ9 Against MCF-7 Cell Line. J Rafsanjan Univ Med Sci 2018; 17 (7): 625-38. [Farsi]
A. Gholami, A. Asadi, A. Abdolmaleki, S. Zahri,
Volume 20, Issue 7 (10-2021)
Abstract
Background and Objectives: Selenium is a trace element and its nanoparticle has antimicrobial activity and due to their low toxicity and excellent biological performance, it has many biological applications in tissue engineering. The aim of this study was to investigate the effect of selenium nanoparticles on stem cell interaction on rat decellularized sciatic nerve scaffolds.
Materials and Methods: In this laboratory study, the effect of selenium nanoparticles on stem cell interaction on rat decellularized sciatic nerve scaffold, scaffolds were prepared using Sondell decellularizaton method and stored in PBS solution containing antibiotics. Biomechanical and histological evaluations of scaffolds were examined using the light microscope. In the next step, adipose stem cells were seeded on the scaffold and the viability of the cells on the scaffold in the presence of selenium nanoparticles were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Data were analyzed by one-way analysis of variance and Tukey's post hoc test.
Results: The tensile test showed that after decellularization, extracellular matrix components such as collagen, laminin, and elastin were retained in the scaffold. Also, DNA content was significantly reduced in the scaffold group (p<0.01). The results of MTT test showed that selenium nanoparticles had no toxicity on stem cells cultured on the scaffold.
Conclusion: The biocompatibility of scaffolds and the viability of stem cells treated with selenium nanoparticles were not significantly different from the control group. Therefore, it can be evaluated as a reinforcing factor to increase the efficiency of scaffolds for the use in regeneration of the nerve lesions.
Key words: Selenium nanoparticles, Stem cell, Decellularized scaffold, Regeneration, Tissue engineering
Funding: This study was funded by University of Mohaghegh Ardabili.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of University of Mohaghegh Ardabili approved the study (IR.UMA.REC.1397.195).
How to cite this article: Gholami A, Asadi A, Abdolmaleki A, Zahri S. Evaluating the Efficiency of Selenium Nanoparticles in the Production of Decellularized Neural Scaffold and the Ability to Preserve Stem Cells Cultured on Them: A Laboratory Study. J Rafsanjan Univ Med Sci 2021; 20 (7): 733-46. [Farsi]
