Showing 13 results for Staphylococcus Aureus
Sh. Assar, A. Jafarzadeh, M. Mohagheghi, R. Bahramabadi,
Volume 4, Issue 3 (6-2005)
Abstract
Antimicrobial Effects of Pinus Eldarica’s Gum and Its Alcoholic Extract on Some Bacteria of Skin Infections
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Sh. Assar MSc [1] , A. Jafarzadeh PhD [2] , M. Mohagheghi MD [3] , R. Bahramabadi BSc [4]
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Background and Objective: Bacterial resistance to antibiotics and synthetic compounds are increasing continously. The aim of this study was to evaluate the antibacterial effects of Pinus eldarica’s gum and its alcoholic extract on Staphylococcus aureus and Escherichia coli which cause some skin infections.
Materials and Methods: After preparing pinus eldarica gum, it was diluted in oleic acid to achieve the concentration of 50% and 75%. The concentration of 50% and 75% of gum’s extract also prepared by soaking the gum in alcoholic solution. Oleic acid and cotrimoxazole were used as control groups. Kirby - bauer method was used as an antibiogram test. Data were analysed by ANOVA variance and Tukey HSD test.
Results: In comparison of the effect of 50% and 75% of gum on E-coli, we observed that there was no remarkable differences between them, but these concentrations of gum were more effective than cotrimoxazole on the inhibition of growth of E-coli. In case of Staphylococcus aureus we observed the negative effect of mentioned concentrations of gum, gum’s extract and cotrimoxazole on its growth, but there was no considerable differences between the different concentration of gums, 50% of gum with 50% and 75% of gum’s extracts and 75% of gum with 50% of gum’s extract on Staphylococci growth.
Conclusion: Overall, the study showed that Pinus eldarica’s gum has strong inhibitory effect on some microbes. This effect can compete with cotrimoxazole on S. aureus and can be effective on cotrimoxazole resistant E-coli.
Key words: Pinus Eldarica, Antimicrobial Effect, Staphylococcus Aureus, E-coli
[1] - (Corresponding author ) Academic Member, Dept. of Microbiology, Rafsanjan, University of Medical Sciences, Rafsanjan
Tel: (0391)5234003, Fax:(0391)5225209, E-mail: assar_sh@yahoo.com
[2] - Assistant Professor, Dept. of Microbiology, Rafsanjan, University of Medical Sciences, Rafsanjan
[3] - General Physician, Rafsanjan, University of Medical Sciences, Rafsanjan
[4] Microbiology Technologist, Rafsanjan, University of Medical Sciences, Rafsanjan
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Volume 5, Issue 4 (12-2006)
Abstract
Background and Objective: Staphylococcus aureus is one of the most important causes of nosocomial infections. Because of increasing rate of antimicrobial resistance, treatment of staphylococcal infections has been made difficult. Therefore, continuous evaluation of Staphylococcus aureus antimicrobial resistance pattern is of great importance.
Materials and Methods: In this descriptive study, we evaluated 131 positive cultures of Staphylococcus aureus isolated from blood, abscess, urine and other body fluids in various wards of the pediatric hospital. The pattern of antibiotic resistance was determined by disk diffusion test. Demographic data and the history of antibiotic therapy analyzed with t-test and qui-square test using SPSS version11.5.
Results: Sensitivity of Staphylococcus aureus to various antibiotics was as follow: vancomycin (98.4%), ceftriaxon (61.5%), amikacin (58.3%) ceftizoxim (52.8%), erythromycin (50%), cephalexin (48.3%) and Amoxicillin (2.6%). Forty eight percent of all cases were due to nosocomial infections. The relation between nosocomial infections and the antibiotic resistance was statistically significant.
Conclusion: Increased rate of resistance to cephalexin, erythromicin and amikacin in this study may be due to the presence of methicillin resistant Staphylococcus aureus or the overuse of these antibiotics. Isolation of two cases of vancomycin resistant Staphylococcus aureus emphasizes on the appropriate use of this antibiotic. Evaluation of vancomycin resistance Staphylococcus aureus by the other standard methods is recommended.
Key words: Staphylococcus aureus, Antimicrobial Resistance, Antibiotic
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Volume 8, Issue 1 (6-2009)
Abstract
Background and Objectives: Staphylococcus aureus carriers are among the important causes of nosocomial infections. According to the studies, medical and health workers have always been considered as a source of nosocomial infections. The aim of this study is determination of the prevalence of the nasal carriers of staphylococcus aureus and antibiotic resistance in clinical wards' staffs in Ali- Ebne Abitaleb hospital, in Rafsanjan.
Material and Methods: This cross- sectional study was done in clinical wards staffs, of Ali- Ebne Abitaleb hospital. Two handred and twenty people between 22 and 58 years old were studied. Sampels were obtained by sterile swabs from deep tissue of anterior nares and then cultured. For identification of staphylococcus aureus, the major tests of catalase, coagolase and DNase were done. Disks of penicillin, cefixim, vancomycin, oxacillin, erythromycin, clindamycin, cefazolin, nitrofurantion, co- trimoxozole, ciprofloxacin, cephalothin doxycyclin and methicillin were used for antibiogram. Data were analyzed using SPSS. Chi- Square and T tests were used where appropriate.
Results: Among the 220 staffs who were studied, 44(20%) were nasal carriers of staphylococcus aureus. Resistance to antibiotics were as follows: penicillin (100%), cefixim(100%), methicillin (97.7%), oxacillin (77.3%) erythromycin (11.4%), doxycyclin (9.1%), cephalotin (6.9%), ciprofloxacin (6.8%), co- trimoxozole (4.5%), clindamycin (2.3%), cefazolin (2.3%) and nitrofurantoin and vancomycin(sensitive).
Conclusion: Considering the remarkable number of nasal carriers of staphylococcus aureus, and specially the high resistance to methicillin, oxacillin and penicillin among hospital staff, in order to prevent nosocomial infections we suggest that the carriers among the staff should be identified and treated properly.
Key words: Nasal Carriers, Staphylococcus Aureus, Hospital Staff, Drug Resistance
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.
A. Salari Sharif, M. Sattari, M. Moradi, R. Shahrokhabad,
Volume 11, Issue 2 (6-2012)
Abstract
Background and Objectives : Staphylococcus aureus is the positive gram coccus which is able to cause different kinds of infection in certain condition. The function of this bacteria is to provide the conditions for the invasion of it to the host with the secretion of different sorts of toxins such as entrotoxin. A and B entrotoxins have the most infections effect among these toxins. that’s why, this experiment for the has been conducted for the detection of entrotoxin genes A and B in clinical samples.
Materials and Method: This cross-sectional and descriptive study, which was conducted from September 2007 to August 2008. Two handred and forty clinical samples from wound, wound, blood, ear, nose, vomited secretions and urine were separation investigated (forty from each sample) and taken from the patients. then after the culture of the samples and conducting confirming biochemical tests, then they were assessed by Poly Chain Reaction technique.
Results: After the diagnosis of this bacteria and conducting objective test, from two hundred and forty samples examined throughout this study, fifty ones (20.83%) were infected by Staphylococcus aureus. Thirty seven cases (74%) of these infected samples were carrying both entrotoxin A and B genes. The frequencies of A and B genes were 11(22%) and 2 (4%), respectively.
Discussion: This study identified that 20.83% of the patients were carrying the pathogen as the source of infection. The detection of entrotoxin A and B genes, shows the most important role they have in bringing about superinfection.
Key words: Staphylococcus Aureus, Gene, Enterotoxins, PCR
Funding: This research was funded by Islamic Azad University Qom Branch.
Conflict of Interest: Not declared.
Ethical approval: The Ethics Committee of Islamic Azad University Qom Branch approved the study.
How to cite this article : Salari Sharif A, Sattari M, Moradi M, Shahrokhabad R . Detection of Staphylococcus aureus Entrotoxin Genes A & B in Clinical Samples of the Patients Referring to the Medical Centers of Kerman and Rafsanjan Cities by PCR Technique . J Rafsanjan Univ Med Scie 2012 11(2): 128-36. [Farsi]
R. Abbaslou, M. Kargar, A. Najafi,
Volume 13, Issue 12 (3-2015)
Abstract
Background
and Objective: Due
to low quantity of pathogens in foods, designing a broth medium to enrich
simultaneously several pathogens through a suitable time to a detectable level
is one of point of interest of many microbiologists. This study aimed to enrich
simultaneously three food-borne pathogens (Listeria monocytogenes,
Staphylococcus aureus and Enterohaemorrhagic Escherichia coli)
transferred by milk products.
Materials and Methods: This experimental
study was carried out in Kerman in 2013. First, a mixture of five enrichment
media, including TSB, TB, TSBYE, SEB and TSB (NaCl 1.5%) was provided. These
mediums were evaluated to enrich of L.monocytogenes, S.aureus and
Enterohaemorrhagic E. coli using culture method. Then sterilized and
pasteurized milk samples were artificially contaminated with these bacteria,
separately and in a mixture. The enrichment ability of these five broth media
was evaluated in three times (8, 18 and 24 hours).
Results: All five media were
able to enrich the pathogens in both single and triple contaminated tubes. The
bacteria in the contaminated milk were enriched through 8 hours, probably due
to use of the nutrients in the milk. However, growth of Listeria
monocytogenes and Staphylococcus aureus were inhibited in the
mentioned condition due to the inhibitory components of milk.
Conclusion: Based on results of
this study, incubation of the bacteria in SEB broth for 18 hours showed the
highest simultaneous enriching ability. Furthermore, it is the first report in
which Tryptose Broth was able to show a simultaneous enriching ability for
these pathogens.
Key words: Simultaneous, Listeria
monocytogenes, Staphylococcus aureus, Enterohaemorrhagic
Escherichia coli
Funding: This research was funded by
personal costs.
Conflict of interest: None declared.
Ethical
approval: The
Ethics Committee of Kerman University approved the study.
How
to cite this article: Abbaslou R, Kargar M,
Najafi A. Efficiency Assessment of Simultaneous Media to
Enrich Listeria Monocytogenes, Staphylococcus Aureus
and Enterohaemorrhagic Escherichia Coli.
J RafsanjanUniv Med Sci 2015 13(12): 1105-14.
[Farsi]
S. Alizadeh, K. Amini,
Volume 14, Issue 5 (7-2015)
Abstract
Background
and Objective: Staphylococcus aureus is a major pathogen of human in the world.
Methicillin-resistant Staphylococcus aureus is associated with
nosocomial infections. Recently, staphylococcal infections have been associated
with the community. The purpose of this study was to isolate and identify Staphylococcus
aureus virulence Panton-Valentine leukocidin (PVL) and methicillin resistance genes in clinical
samples using PCR techniques.
Materials
and Methods:In this experimental study after
collecting samples, it was performed biochemical and microbial tests and then antibiotic
susceptibility testing by disk diffusion method according to CLSI guidelines
with antibiotics of different groups. To identify the virulence and resistance
genes multiplex PCR test was used.
Results: The findings showed that the prevalence of methicillin-resistant
gene (mecA) in clinical samples are 50% and PVL 2.5%, respectively. Among the
antibiotics, resistance to azithromycin, linezolid,
ofloxacin and methicillin were 32.5%, 35%,
27.5% and 27.5%, respectively.
Conclusion :There is a serious threat for
public health because of the importance of Staphylococcus aureus as a
major pathogen in developing countries and due to the increasing use of
antibacterial agents. Multiplex PCR is a simple, sensitive, inexpensive,
relatively quick, and very unique test to identify multiple of genes,
simultaneously.
Keywords:
Staphylococcus aureus, mec A, Panton-Valentine
Leukocidin, Multiplex PCR
Funding:
This research
was funded by Sirjan Science and Research Branch, Islamic Azad University of Sirjan.
Conflict
of interest: None
declared.
Ethical
approval: None declared.
How to cite this article: Alizadeh
S, Amini K. Identification of
Virulence Gene Panton-Valentine Leukocidin (PVL) and Resistance to Methicillin (mecA) in Staphylococcus
Aureus Isolated from Clinical Specimens: A Short Report. J Rafsanjan Univ
Med Sci 2015 14(5): 427-34.
[Farsi]
Z. Kord, K. Amini, M. Parviz,
Volume 14, Issue 8 (11-2015)
Abstract
Background and Objective: Panton-Valentine leukocidin (PVL) toxin is a hemolytic exotoxin that increases the permeability of the cell membrane, thus causing lysis of leukocytes and tissue necrosis. Collagen-binding protein (Cna) adhesion Staphylococcus aureus is responsible for binding to collagen and the major virulence factor in arthritis and osteomyelitis. The purpose of this study was to investigate the presence of antibiotic resistance and virulence genes for PVL, Cna in Staphylococcus aureus strains isolated from clinical specimens by Multiplex PCR.
Materials and Methods: In this cross-sectional, descriptive study, 67 samples of Staphylococcus aureus were isolated. Based on CLSI antimicrobial susceptibility guidline, different antibiotics were tested. Multiplex PCR assay was performed.
Results: Finndings showed that the susceptibility to vancomycin and linezolid antibiotics was 97.1% and maximum resistance is to clindamycin was 23.9%. the frequency of Cna gene in clinical samples was 41.7% while PVL genes were not detected in the samples.
Conclusion: Because of the importance of methicillin resistance Staphylococcus aureus as the cause of infection among people, especially resistant hospital infections, due to increasing use of antibiotics, knowledge of the level of resistance to these agents is essential. Multiplex PCR is a simple, sensitive, inexpensive, relatively quick and very specific in addition, it has an ability to identify multiple genes simultaneously.
Key words: Staphylococcus aureus, Panton -Valentine leukocidin, Cna, Multiplex PCR
Funding: This research was funded by Saveh Science and Research Branch, Islamic Azad University, Saveh, Iran
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Saveh Science and Research Branch, Islamic Azad University approved the study.
How to cite this article: Kord Z, Amini K, Parviz M. Determining the Frequency of Panton-Valentine Leukocidin (PVL), and Collagen- Binding protein (can) in Staphylococcus Aureus Strains Isolated from Clinical Specimens and Antibiotic Resistance: A Short Report. J RafsanjanUniv Med Sci 2015 14(7): 703-10. [Farsi]
Gh . Shojaei, A. Akhavan Sepahy, R.i Rafiei Tabatabae, K. Tahvildari,
Volume 17, Issue 2 (5-2018)
Abstract
Background and Objectives: Expression of genes related to biofilm formation in
methicillin-resistant staphylococcus aureus (MRSA) can play an effective role in biofilm formation and pathogenicity. Due to the significance of antimicrobial activity of Artemisia species against biofilm producer genes, this study aimed to evaluate the effect of ethanolic extract of Artemisia oliveriana on the expression of icaA, icaD, and ebps genes in
MRSA.
Materials and Methods: This was a laboratory study. First, Artemisia oliveriana extract was prepared and then, the minimum inhibitory concentration (MIC) of growth for the extract of the plant against
s. aureus strains containing mecA was obtained by microdilution method . Then, at the lower concentrations, levels of expression in the icaA, icaD, and ebps genes in
MRSA were determined by Real-Time PCR, and the results were analyzed through repeated measures ANOVA.
Results: The MIC of ethanolic extract of A. oliveriana was 512 μg/ml. The reduction of the expression of the genes in the standard sample after 4, 8, and 16 hours was significant in the Artemisia oliveriana group (p<0.05).
Conclusion: The ethanolic extracts of Artemisia oliveriana had a significant effect on the expression of the genes involved in biofilm formation and this expression reduction was not seen in the housekeeping DNA gyrase-B as a reference gene.
Key words: Artemisia oliveriana, Biofilm, Real-Time PCR, Staphylococcus aureus, Methicillin
Funding: This study did not have any funds.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University, North Tehran Branch approved the study.
How to cite this article: Shojaei Gh, Akhavan Sepahy A, Rafiei Tabatabaei R, Tahvildari K. The Effects of Ethanolic Extract of Artemisia oliveriana on Expression of
icaA, icaD, and ebps Genes in
MRSA: A Short Report.
Univ Med Sci 2018; 17 (2): 169-76. [Farsi]
Z. Shakeran, Z. Shakeran, M. Nosrati,
Volume 18, Issue 2 (5-2019)
Abstract
Background and Objectives: Currently infections caused by Staphylococcus aureus have increased and raised antibiotic resistant isolates. Therefore, lots of studies have been done to introduce novel anti-S.aureus compounds especially natural originated constitutes. Hence, this study aimed to determine bioinformatics screening of novel inhibitors of antibiotic resistance-associated proteins in S.aureus from medicinal plants.
Material and Methods: Firstly, in this descriptive study, three-dimension structure of a set of phytochemicals from three medicinal plants including Ferulago angulat, Scrophularia striata, Laurus nobilis were retrieved from PubChem database. Then, three-dimension structure of four antibiotic resistance-associated proteins including β-lactamase, penicillin binding protein 2A (PBP2a), Vancomycin A (VanA) and D-alanyl-D-alanine dipeptidase (VanX) were obtained from Protein Data Bank. Finally, the ability of the selected phytocompounds for inhibition of the mentioned antibiotic resistance-associated proteins was investigated using molecular docking method by iGemdock 2.1 software.
Results: The results showed that most of the tested compounds had appropriate interaction to all the studied proteins especially to VanX. The results also revealed that Bis (2-ethylhexyl) phthalate and Palmitic acid had strongest interactions to all the tested proteins.
Conclusion: The results of this study showed that Bis (2-ethylhexyl) phthalate and Palmitic acid found in the surveyed medicinal plants are good candidates for more in vitro and in vivo studies about anti-bacterial activity against antibiotic resistant S. aureus.
Keywords: Staphylococcus aureus, Antibiotic resistance, Medicinal plants, Bioinformatics.
Funding: None declared.
Conflict of interest: None declared.
Ethical approval: None declared.
How to cite this article: Shakeran Z, Shakeran Z, Nosrati M. Bioinformatics Study of the Effects of Some Phytocompounds Found in Ferulago Angulat, Scrophularia Striata and Laurus Nobilis Medicinal Plants on Inhibition of the Proteins Causing Antibiotic Resistance in Staphylococcus Aureus: A Descriptive Study. J Rafsanjan Univ Med Sci 2019; 18 (2): 177-92. [Farsi]
F. Saffari, A> Radfar, M. H. Sobhanipoor, R. Ahmadrajabi,
Volume 18, Issue 3 (6-2019)
Abstract
Background and Objectives: Colonization of Staphylococcus aureus in the nose of healthcare workers (HCWs) can be considered as the main source of nosocomial infections. The aim of this study was to investigate the frequency of S. aureus nasal carriage among HCWs in Pastor and Aflatoonian hospitals in Bam, its antibiotic resistance patterns, and determine Staphylococcal protein A (spa) typing and staphylococcal cassette chromosome mec (SCCmec) typing of methicillin resistant S. aureus isolate.
Materials and Methods: In this cross-sectional study, a total of 258 nasal swabs were collected from HCWs during 2017 and were analyzed to detect methicillin sensitive and resistant S. aureus isolates. Resistance to different antibiotic agents was determined by disk diffusion. The presence of nuc, mecA, pvl genes, and spa and SCCmec types were determined by polymerase chain reaction technique (PCR). The data were analyzed using chi-square and Fisher's exact tests.
Results: Thirty seven subjects (14.3%) were nasal carrier of S. aureus and one of them (2.7%) was resistant to methicillin with Sccmec type IV and spa type t608. All of isolates were sensitive to vancomycin, linezolid and mupirocin. The pvl gene was found in 8 (22.2%) isolates. Except with regard to gender (p=0.036), occupation, age, hospital ward, years of healthcare service, previous antibiotic therapy during the last three months, hospitalization and blood group were not significantly associated with nasal carriage (p˃ 0.05).
Conclusion: Although according to the results, the frequency of nasal carriage of S. aureus isolates among HCWs at our hospitals compared to the other studies from Iran was low, regular S. aureus carriage screening as well as decolonization strategies is recommended.
Key words: Staphylococcus aureus, Drug resistance, Health personnel, Molecular typing, Bam
Funding: This research was funded by Research Council of Bam University of Medical Sciences with the grant Number of 97/96.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Bam University of Medical Sciences approved the study (IR. MUBAM. REC. 1397.003).
How to cite this article: Saffari F, Radfar A, Sobhanipoor MH, Ahmadrajabi R. Frequency of Nasal Carriage of Staphylococcus aureus and Associated Risk Factors and Its Antibiotic Susceptibility Pattern Among Healthcare Workers in Pastor and Aflatoonian Hospitals in Bam, Southeast Iran, in 2017: A Cross-Sectional Study. J Rafsanjan Univ Med Sci 2019; 18 (3): 209-26. [Farsi]
H. Ebrahimi, R. Mahmoudi, P. Ghajarbeygi, S. Shasavari, Sh. Mossavi, N Biglari,
Volume 18, Issue 7 (9-2019)
Abstract
Background and Objectives: The aim of this study was to determine the prevalence of Methicillin-Resistant Staphylococcus aureus in traditional and device-made ice cream in Qazvin.
Materials and Methods: In this cross- sectional study, 100 samples of traditional and device-made ice cream in Qazvin were randomly collected over a period of one year and in different seasons. The culture method was then confirmed by PCR (Polymerase Chain Reaction) technique.
Results: The inflammation rate of Staphylococcus aureus bacteria was 5% in most seasons in the traditional and device-made ice cream and the most inflammation was in summer, and 80% (4 cases) of the isolated bacteria were methicillin resistant.
Conclusion: The use of pasteurized ice cream and observance of health principles in producing traditional and device-made ice cream will reduce the risk of contamination and food poisoning.
Key words: Staphylococcus aureus, Traditional ice cream, Device-made ice cream, PCR, Antibiotic resistance, Qazvin
Funding: This research was funded by Qazvin University of Medical Sciences, Qazvin, Iran.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Qazvin University of Medical Sciences approved the study (Ethic Nubmer: IR.QUMS.REC.1396.352).
How to cite this article: Ebrahimi H, Mahmoudi R, Ghajarbeygi P, Shahsavari S, Mousavi Sh, Biglarikhoshmaram N. Study of Methicillin-Resistant Staphylococcus Aureus in Traditional and Device-Made Ice Cream in Qazvin, 2017-2018
: A Short Report.
J Rafsanjan Univ Med Sci 2019; 18 (7): 721-8. [Farsi]
Faezeh Amiri, Nadia Kazemipour,
Volume 22, Issue 3 (6-2023)
Abstract
Background and Objectives: Antibiotic resistance is known to be the main challenge against treatment of infectious diseases related to Staphylococcus aureus (S.aureus). Niosomal nanosystems are the new drug delivery systems which enhance antimicrobial potential activity of antibiotics. The aim of this study was to determine the effect of a niosomal drug delivery system containing gentamicin against S.aureus.
Materials and Methods: In this laboratory study, different niosomes including Span 60, Tween 60, and cholesterol in combination with gentamicin with different molar ratio were synthesized using the thin film hydration method. Synthesized niosomal nanosystems were identified by light microscope and dynamic laser light scattering (DLS) technique. Antibacterial activity of synthesized niosomes against S.aureus (PTCC: 1112) was evaluated by well diffusion and minimum inhibitory concentration (MIC) methods. Data was analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test.
Results: Niosomal nanosystems were identified in spherical vesicles and in 3 formulations with average particle sizes of 9.09±0.76, 27.59±15.20 and 9.10±0.30 µm, respectively. The results revealed that the formulation with 60:40 molar ratio had higher gentamycin encapsulation efficiency (90.82%) and also, 60:40 niosomal formulation with half the concentration of commercially available gentamicin solution had the same inhibition of growth on Staphylococcus aureus bacteria )p=0.658 (.
Conclusion: Controlled and continuous release of gentamicin from the niosomal nanosystem, along with enhanced drug penetration could reduce the growth of S.aureus. Accordingly, niosomal nanosystem has a good potential to be an effective drug delivery system.
Key words: Staphylococcus aureus, Gentamycin, Niosome
Funding: This study did not have any funds.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University, Kerman Branch, approved the study (IR.IAU.KERMAN.REC.1401.009).
How to cite this article: Amiri Faezeh, Kazemipour Nadia. The Effect of Niosomal Nanosystem Containing Gentamicin on the Staphylococcus Aureus: A Laboratory Study. J Rafsanjan Univ Med Sci 2023; 22 (3): 223-36. [Farsi]
Abdolreza Babaee, Ebrahim Rezazadeh Zarandi, Ahmad Shabanizadeh, Zahra Taghipour, Samereh Dehghani-Soltani, Mahnaz Ramezani, Amirhesam Khodaei, Mohammad Mohsen Taghavi,
Volume 23, Issue 6 (10-2024)
Abstract
Background and Objectives: The aim of this study was to investigate the effect of sheep lymph nodes and antibiotics on the bacterium that causes acne and the bacteria that usually exist with it.
Materials and Methods: In this laboratory study, 24 patients with acne (18 to 45 years old) were selected to isolate the bacteria. Also, 24 clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis were isolated from the wound samples of patients. After culturing the bacteria, their sensitivity to common antibiotics was evaluated. Data were reported as percentage.
Results: The results showed that Cutibacterium acnes and Staphylococcus aureus were 100% sensitive to vancomycin. Also, Cutibacterium acnes showed sensitivity in tetracycline and imipenem groups in 95% of cases and in cotrimoxazole and piperacillin/tazobactam groups in 90% of cases. In addition, the highest sensitivity of Staphylococcus epidermis to piperacillin/tazobactam (62.5%) and the highest drug resistance (91.67%) were related to azithromycin and erythromycin groups.
Conclusion: Due to the increase in drug resistance, it is suggested to use antibiotics with the least drug resistance such as vancomycin and imipenem in the treatment of acne.
Keywords: Lymph node, Antibiotic, Acne, Staphylococcus aureus, Cutibacterium acnes
Funding: This study was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical considerations: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1399.103).
Authors’ contributions:
- Conceptualization: Mohammad Mohsen Taghavi, Ebrahim Rezazadeh Zarandi
- Methodology: Mohammad Mohsen Taghavi, Ahmad Shabanizadeh
- Data collection: Mahnaz Ramezani, Amir Hesam Khodaei
- Formal analysis: Abdolreza Babaee, Samereh Dehghani-Soltani
- Supervision: Mohammad Mohsen Taghavi, Zahra Taghipour
- Project administration: Mohammad Mohsen Taghavi
- Writing - original draft: Abdolreza Babaee, Ebrahim Rezazadeh Zarandi
- Writing - review & editing: Mohammad Mohsen Taghavi, Abdolreza Babaee
Citation: Babaee A, Rezazadeh Zarandi E, Shabanizadeh E, Taghipour Z, Dehghani-Soltani S, Ramezani M, Khodaei AH, Taghavi MM. Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report. J Rafsanjan Univ Med Sci 2024; 23 (6): 568-75. [Farsi] |
