fa مهار رگ زایی با زهر زنبور عسل در مدل حلقه آئورت موش صحرایی زيست شناسي زيست شناسي پژوهشي Research <p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 7.1pt margin-left: 7.1pt margin-bottom: 0.0001pt text-align: justify line-height: 120% "><!--stripped--> gte vml 1]><v:roundrect id="_x0000_s1026" style='position:absolute left:0text-align:leftmargin-left:2.45ptmargin-top:1.4ptwidth:449.5pt height:326.25ptz-index:-1' arcsize="1475f"> <w:wrap anchorx="page"/> </v:roundrect><!--stripped--><span style="position: absolute z-index: -1 left: 0px margin-left: 2px margin-top: 1px width: 602px height: 437px "><img alt="AWT IMAGE" width="602" height="437" src="file:///C:/DOCUME~1/ADMINI~1/LOCALS~1/Temp/msohtmlclip1/01/clip_image001.gif" v:shapes="_x0000_s1026" ></span><b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' color: black ">چکیده<o:p /></span></b></p> <p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 7.1pt margin-left: 7.1pt margin-bottom: 0.0001pt text-align: justify line-height: 120% "><b><span lang="AR-SA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' color: black ">زمینه و هدف:</span></b><span lang="AR-SA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' color: black "> </span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">رگ‌زایی( تشکیل رگ‌های خونی جدید از رگ‌های قبلی)، یک فرآیند ضروری در تکوین محسوب می‌شود. زهر زنبور عسل سال‌هاست که در درمان بیماری‌های مختلف کاربرد دارد.در پژوهش حاضر اثر زهر زنبور عسل بر رگ‌زایی در مدل حلقه آئورت موش صحرایی نژاد ویستار بررسی شده است.<o:p /></span></p> <p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 7.1pt margin-left: 7.1pt margin-bottom: 0.0001pt text-align: justify line-height: 120% "><b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">مواد و روش‌ها: </span></b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">در این مطالعه تجربی- آزمایشگاهی<b> </b>آئورت موش صحرایی به قطعات یک میلی‌متری برش و در ماتریکس کلاژن کشت داده شد. پس از ظاهر شدن نخستین جوانه‌ها نمونه‌ها به گروه‌های شاهد و تجربی (تیمار با غلظت‌های 70،10،20،30،40،50،60 میکروگرم در میلی‌لیتر) تقسیم‌بندی شدند. رگ‌زایی با میکروسکوپ معکوس بررسی و عکس‌برداری شد. تعداد و طول انشعابات رگی توسط نرم‌افزار </span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">Image J</span><span dir="RTL"></span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span> تعیین و داده‌های کمی توسط نرم‌افزار آماری </span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">SPSS</span><span dir="RTL"></span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span> و آزمون‌های آماری </span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">ANOVA</span><span dir="RTL"></span><span style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span> <span lang="FA">و </span></span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">TUKEY</span><span dir="RTL"></span><span style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span> <span lang="FA">در سطح (05/0</span></span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">p<</span><span dir="RTL"></span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span>) تحلیل گردید.<o:p /></span></p> <p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 7.1pt margin-left: 7.1pt margin-bottom: 0.0001pt text-align: justify line-height: 120% "><b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">یافته‌ها: </span></b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">میانگین طول و تعداد عروق خونی در گروه‌های تجربی تیمار با غلظت‌های10،20،30 میکروگرم در میلی‌لیتر نسبت به گروه شاهد تغییرات معنی‌دار نشان نداد (05/0</span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">p></span><span dir="RTL"></span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span>) همچنین در گروه‌های تجربی تیمار با غلظت‌های50،60،70،40 میکروگرم در میلی‌لیتر کاهش معنی‌دار مشاهده شد (05/0></span><span dir="LTR" style="line-height: 120% font-family: 'Times New Roman', serif ">p</span><span dir="RTL"></span><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "><span dir="RTL"></span>).<o:p /></span></p> <p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 7.1pt margin-left: 7.1pt margin-bottom: 0.0001pt text-align: justify line-height: 120% "><b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' ">نتیجه‌گیری:</span></b><span lang="FA" style="font-size: 12pt line-height: 120% font-family: 'B Nazanin' "> غلظت‌های مناسب زهر زنبور عسل اثر مهاری روی رگ‌زایی دارد، لذا می‌تواند در مطالعات مربوط به سرطان و حالات پاتولوژیک وابسته به رگ‌زایی مورد توجه خاص قرار گیرد.<o:p /></span></p> <b><span lang="FA" dir="RTL" style="font-size: 12pt font-family: 'B Nazanin' ">واژه‌های کلیدی: </span></b><span lang="FA" dir="RTL" style="font-size: 12pt font-family: 'B Nazanin' ">رگ‌زایی، زهر، آئورت، سرطان</span><span lang="FA" dir="RTL" style="font-size: 1pt font-family: 'B Nazanin' color: black ">،</span><span lang="FA" dir="RTL" style="font-size: 12pt font-family: 'B Nazanin' color: black ">، زنبور عسل</span> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif color: black ">Background and Objective:</span></b><span style="font-family: 'Times New Roman', serif color: black "> </span><span class="hps"><span style="font-family: 'Times New Roman', serif ">Angiogenesis</span></span><span style="font-family: 'Times New Roman', serif ">- formation <span class="hps">of new blood vessels</span> from<span class="hps"> former ones- is an essential process in development</span>. <span class="hps">Bee venom </span>has been used to cure diverse ills for many years. In <span class="hps">the present research the</span> </span><span style="font-family: 'Times New Roman', serif ">inhibit</span><span class="hps"><span style="font-family: 'Times New Roman', serif ">ory effect</span></span><span style="font-family: 'Times New Roman', serif "> of </span><span style="font-family: 'Times New Roman', serif ">bee venom </span><span style="font-family: 'Times New Roman', serif ">on </span><span style="font-family: 'Times New Roman', serif ">angiogenesis</span><span style="font-family: 'Times New Roman', serif "> has been investigated</span><span style="font-family: 'Times New Roman', serif "> in a wistar rat</span><span style="font-family: 'Times New Roman', serif "> </span><span style="font-family: 'Times New Roman', serif ">aortic model<b>.<o:p /></b></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Materials and Methods:</span></b><span style="font-family: 'Times New Roman', serif "> </span><span class="hps"><span style="font-family: 'Times New Roman', serif ">Rat</span></span><span style="font-family: 'Times New Roman', serif ">'s <span class="hps">aorta</span> was cut into <span class="hps">1</span> <span class="hps">mm<span dir="RTL"></span><span dir="RTL"><span dir="RTL"></span> </span>pieces and cultured in</span> <span class="hps">collagen matrix.<span dir="RTL"></span><span dir="RTL"><span dir="RTL"></span> </span></span>A<span class="hps">fter </span>observation of the first <span class="hps">sprouting</span> of <span class="hps">angiogenesis, the samples were divided into control and experimental groups. Experimental groups were treated with </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">bee venom</span></span><span class="hps"><span style="font-family: 'Times New Roman', serif "> in concentration 10, 20, 30, 40, 50, 60, 70 µg/ml. </span></span><span style="font-family: 'Times New Roman', serif ">Angiogenesis was investigated and photographed by invert microscope. Numbers and lengths of vessels were measured by Image J software. Data were analyzed by SPSS software and ANOVA & TUKEY in significant level (p<0.05).<span lang="AR-SA" dir="RTL"><o:p /></span></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Results: </span></b><span style="font-family: 'Times New Roman', serif ">Lengths average and numbers of<span> </span>blood vessels in <span class="hps">experimental</span><span class="shorttext"> </span><span class="hps">groups at concentration of 10, 20, 30µg/</span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">ml</span></span><span class="hps"><span style="font-family: 'Times New Roman', serif "> in comparison to</span></span><span style="font-family: 'Times New Roman', serif "> control group showed any significant changes (p>0.05)<span dir="RTL"></span><span lang="AR-SA" dir="RTL"><span dir="RTL"></span>.</span><span dir="LTR"></span><span lang="AR-SA"><span dir="LTR"></span> </span><span class="hps">length average and numbers of</span> <span class="hps">blood vessels</span> <span class="hps">in</span> <span class="hps">experimental groups</span> <span class="hps">with 40, 50, 60, 70 µg/ml concentration showed</span> <span class="hps">a significant decrease</span><span class="atn"> in comparison to control group <span dir="RTL"></span><span lang="AR-SA" dir="RTL"><span dir="RTL"></span>)</span>p<0.05).<o:p /></span></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Conclusion: </span></b><span class="hps"><span style="font-family: 'Times New Roman', serif ">The proper</span></span><span style="font-family: 'Times New Roman', serif "> <span class="hps">concentrations of</span> <span class="hps">BV inhibits</span> <span class="hps">angiogenesis</span></span><span style="font-family: 'Times New Roman', serif ">.</span><span class="hps"><span style="font-family: 'Times New Roman', serif "> So</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> it</span></span><span class="hps"><span style="font-family: 'Times New Roman', serif "> can</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">be considered</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">in studies of</span></span><a name="_GoBack"></a><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">cancer and</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">pathological conditions</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">associated with</span></span><span class="longtext"><span style="font-family: 'Times New Roman', serif "> </span></span><span class="hps"><span style="font-family: 'Times New Roman', serif ">angiogenesis.</span></span><span style="font-family: 'Times New Roman', serif "><o:p /></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><span class="hps"><b><span style="font-family: 'Times New Roman', serif ">Key words</span></b></span><b><span style="font-family: 'Times New Roman', serif ">:</span></b><span style="font-family: 'Times New Roman', serif "> <span class="hps">Angiogenesis</span>, <span class="hps">Venom</span>, Aorta, Cancer, Bee<span lang="AR-SA" dir="RTL"><o:p /></span></span></p> <p class="MsoNormal" style="text-align: left line-height: 150% direction: ltr unicode-bidi: embed "><span lang="FA" dir="RTL" style="font-family: 'Times New Roman', serif "><!--stripped--> <!--stripped--></span></p> <p class="MsoNormal" style="text-align: left line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Funding:</span></b><span style="font-family: 'Times New Roman', serif "> This research was funded by Islamic Azad University of Mashhad.<b><o:p /></b></span></p> <p class="MsoNormal" style="text-align: left line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Conflict of Interest:</span></b><span style="font-family: 'Times New Roman', serif "> None declared.<b><o:p /></b></span></p> <p class="MsoNormal" style="text-align: left line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Ethical approval:</span></b><span style="font-family: 'Times New Roman', serif "> The Ethics Committee of Islamic Azad University, Mashhad Branch, approved the study.<span lang="AR-SA" dir="RTL"><o:p /></span></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif color: black "><!--stripped--> <!--stripped--></span></b></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif color: black ">How to cite this article:</span></b><b><span style="font-size: 12pt line-height: 150% font-family: 'Times New Roman', serif color: black "> </span></b><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif ">Baharara J</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif ">, Hosseini N, Ramezani T. Angiogenesis Inhibition by Bee Venom in Rat Aortic Model.</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black "> <i>J RafsanjanUniv Med Sci </i></span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black ">2014 13(4): 361-70</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black ">. [Farsi]<o:p /></span></p> <p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><span style="font-family: 'Times New Roman', serif color: black "><!--stripped--> <!--stripped--></span></p> رگ‌زایی, زهر, آئورت, سرطان,, زنبور عسل Angiogenesis, Venom, Aorta, Cancer, Bee 361 370 http://journal.rums.ac.ir/browse.php?a_code=A-10-1666-1&slc_lang=fa&sid=1 J. Baharara جواد بهارارا baharara@yahoo.com 6900319475328460012960 6900319475328460012960 Yes Mashhad Branch, Islamic Azad University دانشگاه آزاد اسلامی مشهد N. Hosseini نسرین حسینی 6900319475328460012961 6900319475328460012961 No Mashhad Branch, Islamic Azad University دانشگاه آزاد اسلامی مشهد T. Ramezani طیبه رمضانی 6900319475328460012962 6900319475328460012962 No kharazmi university دانشگاه خوارزمی