Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.61186/jrums
fa
jalali
1393
11
1
gregorian
2015
2
1
13
10
online
1
fulltext
fa
کاربرد میکرو ذرات چیتوزان – تکنسیوم-99 در تصویربرداری ریه موش: یک گزارش کوتاه
The Application of 99mTc-Chitosan Microparticles in Lung Imaging of Mice: A Short Report
فيزيك پزشكي
Physics
پژوهشي
Research
<div align="justify" style="direction: rtl "><p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><!--stripped--> gte vml 1]><v:roundrect
id="_x0000_s1026" style='position:absoluteleft:0text-align:left
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</v:roundrect><!--stripped--><span style="position: relative z-index: -1 "><span style="position: absolute left: 6px top: -1px width: 602px height: 338px "><img alt="AWT IMAGE" width="602" height="338" src="file:///C:/DOCUME~1/ADMINI~1/LOCALS~1/Temp/msohtmlclip1/01/clip_image001.gif" v:shapes="_x0000_s1026" ></span></span><b><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' color: black ">چکیده</span></b><b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' color: black "><o:p /></span></b></p>
<p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' color: black ">زمینه و هدف:</span></b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' color: black "> </span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">اسکن پرفیوژن ریه، در پزشکی هستهای به کمک </span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">آلبومین<b> </b>ماکروآگرگیته<b>-</b></span><sup><span dir="LTR" style="font-size: 12pt line-height: 125% font-family: 'Times New Roman', serif ">m</span></sup><span dir="LTR" style="font-size: 12pt line-height: 125% font-family: 'Times New Roman', serif ">Tc</span><span dir="RTL"></span><sup><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "><span dir="RTL"></span>99 </span></sup><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">که ذراتی نشاندار است انجام میشود. مشکلات و سختیهایی حین
ساخت این ذرات وجود دارد</span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> که</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">
برای رفع این معضل، از میکروذرات چیتوزان به عنوان جایگزین استفاده شد و بعد از
خالص سازی با تکنسیوم نشاندار گردید.<o:p /></span></p>
<p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><b><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">مواد و روشها</span></b><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">:</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">
تستهای </span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">کنترل کیفی اعم از تعیین اندازه ذرات، </span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">درصد
نشاندارسازی</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">، پایداری در بافر</span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> سالین و</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">
تصویربرداری با سیستم پزشکی هستهای در بدن موش انجام شد.<o:p /></span></p>
<p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">یافتهها:</span></b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> اندازه ذرات 90-110 میکرون، درصد نشاندارسازی </span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">3±94%، پایداری بالا در بافر سالین و
تجمع بسیار خوب در ریههای موش مشاهده شد.<o:p /></span></p>
<p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">نتیجهگیری:</span></b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> نتایج نشان داد که میکروذرات
چیتوزان-تکنسیوم99</span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> احتمالا میتواند</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> یک
کاندیدای امیدوارکننده برای کاربرد تشخیصی در اسکن پرفیوژن ریه بکار گرفته شود که
نیاز به مطالعات بیشتری دارد.</span><span dir="LTR" style="font-size: 12pt line-height: 125% font-family: 'Times New Roman', serif "><o:p /></span></p>
<p class="MsoNormal" dir="RTL" style="margin-top: 0cm margin-right: 2.85pt margin-left: 10.5pt margin-bottom: 0.0001pt text-align: justify line-height: 125% "><b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">واژههای کلیدی:</span></b><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> چیتوزان، اسکن پرفیوژن ریه</span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">،</span><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "> آلبومین<b> </b>ماکروآگرگیته<b>-</b></span><sup><span dir="LTR" style="font-size: 12pt line-height: 125% font-family: 'Times New Roman', serif ">m</span></sup><span dir="LTR" style="font-size: 12pt line-height: 125% font-family: 'Times New Roman', serif ">Tc</span><span dir="RTL"></span><sup><span lang="AR-SA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' "><span dir="RTL"></span>99</span></sup><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' ">، تکنسیوم</span><span lang="FA" style="font-size: 12pt line-height: 125% font-family: 'B Nazanin' color: black "><o:p /></span></p></div>
<p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif color: black ">Background and </span></b><b><span style="font-family: 'Times New Roman', serif ">Objective:</span></b><span style="font-family: 'Times New Roman', serif "> In nuclear medicine, lung perfusion
scintigraphy is performed by radiolabeled particles,<a href="http://www.google.com/url?url=http://en.wikipedia.org/wiki/Technetium_(99mTc)_albumin_aggregated&rct=j&frm=1&q=&esrc=s&sa=U&ei=ZxLGU8joD-bW0QWbkICYDg&ved=0CBcQFjAA&usg=AFQjCNH3qcIS9UZXk5_GPa2Xa5ZlJJ6CRA" target="_blank"><sup> </sup>99<sup> </sup>mTc-albumin <span class="st1"><span style="color: windowtext text-decoration: none ">macroaggregated.
Synthesizing of the particles has some difficulties. In order to resolve this
problem, chitosan microparticles were used as an alternative choice.</span></span><span style="color: windowtext text-decoration: none "> </span></a><o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Materials
and Methods:</span></b><span style="font-family: 'Times New Roman', serif "> The
qualitative control tests including: the determining particles size, the
labeling efficiency and stability in saline buffer and imaging studies in the
mice were performed.<o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Results:</span></b><span style="font-family: 'Times New Roman', serif "> The size of particles was 90-110
micron. The labeling efficiency was 94±3%, the good stability in saline buffer
and high accumulation of activity in mice lung were seen.<o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Conclusion:</span></b><span style="font-family: 'Times New Roman', serif "> The results showed that t</span><span style="font-family: 'Times New Roman', serif ">he </span><span style="font-family: 'Times New Roman', serif ">99mTc-chitosan microparticles may
be considered as a promising radiopharmaceutical of lung perfusion scintigraphy
that needs more investigations.<b><o:p /></b></span></p>
<p class="MsoNormal" style="text-align: justify direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif ">Key
words: </span></b><span style="font-family: 'Times New Roman', serif ">Chitosan,</span><span style="font-family: 'Times New Roman', serif "> Lung perfusion scintigraphy,</span> <span style="font-family: 'Times New Roman', serif ">99mTc-albumin macroaggregated, 99mTc</span><b><span style="font-family: 'Times New Roman', serif "><o:p /></span></b></p>
<p class="MsoNormal" style="text-align: justify text-indent: 14.2pt line-height: 180% direction: ltr unicode-bidi: embed "><span lang="AR-SA" dir="RTL" style="font-size: 6pt line-height: 180% font-family: 'B Nazanin' "><!--stripped--> <!--stripped--></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 180% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">Funding:</span></b><span dir="RTL"></span><span dir="RTL" style="font-family: 'Times New Roman', serif "><span dir="RTL"></span> </span><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">This research
was not funded by any university or organization.</span><span style="font-family: 'Times New Roman', serif "><o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 180% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">Conflict of Interest:</span></b><span dir="RTL"></span><span dir="RTL" style="font-family: 'Times New Roman', serif "><span dir="RTL"></span> </span><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">Not declared</span><span style="font-family: 'Times New Roman', serif ">.<o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 180% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">Ethical approval:</span></b><span dir="RTL"></span><span dir="RTL" style="font-family: 'Times New Roman', serif "><span dir="RTL"></span> </span><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">This research
was performed based on the Ethics Committee of</span><span dir="RTL"></span><span dir="RTL" style="font-family: 'Times New Roman', serif "><span dir="RTL"></span> </span><span style="font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-origin: initial background-clip: initial background-color: white background-position: initial initial background-repeat: initial initial ">Nuclear science
and Technology institute, Helsinki ethic.</span><span style="font-family: 'Times New Roman', serif "><o:p /></span></p>
<p class="MsoNormal" style="text-align: justify line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-size: 6pt line-height: 150% font-family: 'Times New Roman', serif color: black "><!--stripped--> <!--stripped--></span></b></p>
<p class="MsoNormal" style="text-align: left line-height: 150% direction: ltr unicode-bidi: embed "><b><span style="font-family: 'Times New Roman', serif color: black ">How to cite this article:</span></b><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif ">
Rasaneh S,</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif "> Johari-Daha F</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif ">. The Application of 99mTc-Chitosan
Microparticles in Lung Imaging of Mice: A Short Report</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif ">. </span><i><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black ">J RafsanjanUniv Med Sci </span></i><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black ">2015 13(10): 1013-18.</span><span style="font-size: 9pt line-height: 150% font-family: 'Times New Roman', serif color: black "> [Farsi]<o:p /></span></p>
<!--EndFragment--><br><p></p>
چیتوزان, اسکن پرفیوژن ریه, آلبومین ماکروآگرگیته-mTc99, تکنسیوم
Chitosan, Lung perfusion scintigraphy, 99mTc-albumin macroaggregated, 99mTc
1013
1018
http://journal.rums.ac.ir/browse.php?a_code=A-10-1867-1&slc_lang=fa&sid=1
S.
Rasaneh
سمیرا
رسانه
samira_rasaneh@hotmail.com
6900319475328460014604
6900319475328460014604
Yes
Nuclear Science and Technology Research Institute
پژوهشگاه علوم و فنون هسته ای
F.
Johari-Daha
فریبا
جوهری دها
6900319475328460014605
6900319475328460014605
No