fa مقایسه اثر دهانشویه بابونه و کلرهگزیدین 2/0 درصد بر روی التیام زخم‌های دهانی در موش صحرایی نر: یک مطالعه تجربی فيزيولوژي Physiology پژوهشي Research <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چکیده</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زمینه و هدف</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">:</span></span></strong> <span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">از آنجایی که وجود زخم دهانی باعث اختلال در عملکرد‌های طبیعی دهان فرد می‌شود، بنابراین یافتن راه‌هایی برای تسریع روند بهبود زخم، ضروری به نظر می‌رسد. بابونه (</span></span></span><em><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">Matricaria chamomilla</span></span></em><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">) گیاه دارویی است که دارای اثرات مفیدی در ترمیم انواع زخم‌ها می‌باشد. این مطالعه به منظور تعیین و مقایسه اثر دهانشویه عصاره بابونه با کلرهگزیدین 2/0 درصد بر روند بهبود زخم‌های برشی در موش صحرایی نر انجام شد.</span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">مواد و روش‌ها:</span></span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> در این مطالعه تجربی، تعداد 24 موش صحرایی نر، انتخاب و تحت بیهوشی عمومی‌قرار گرفتند. سپس زخم برشی در ناحیه مرکزی زبان، به عمق یک میلی‌متر و محدود به مخاط ایجاد شد. حیوانات به صورت تصادفی به دو گروه کنترل (دهانشویه کلرهگزیدین) و هدف (دهانشویه عصاره بابونه) تقسیم ‌شدند. هر گروه به صورت تصادفی به سه زیرگروه تقسیم و حیوانات در روزهای سوم، هفتم و دهم کشته ‌شدند. سپس از ناحیه زبان نمونه تهیه و توسط پاتولوژیست از نظر وسعت بافت گرانولاسیون، آنژیوژنز، میانگین تعداد فیبروبلاست‌ها، نوتروفیل‌ها، لنفوسیت‌ها و ماکروفاژها بررسی شدند و نتایج ثبت گردید. داده‌ها با آزمون من-ویتنی تجزیه و تحلیل شدند.</span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">یافته‌ها: </span></span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">هر دو دهانشویه جهت تسریع روند ترمیم به یک میزان عمل کردند و به جز درصد ماکروفاژ‌ها در روز سوم (028/0=</span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">P</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">) دیگر متغیر‌های مورد بررسی، تفاوت معنی‌داری را نشان ندادند (05/0<</span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">P</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">).</span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">نتیجه‌گیری: </span></span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">با توجه به اثرات مشابه دهانشویه‌های عصاره بابونه و کلرهگزیدین (به عنوان درمان استاندارد) در بهبود زخم، و هم‌چنین اثرات جانبی کم‌تر دهانشویه‌های گیاهی، می‌توان دهانشویه بابونه را به جای کلرهگزیدین جهت تسریع روند ترمیم زخم برشی پیشنهاد کرد.</span></span></span><br> <strong><span dir="RTL" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">واژه‌های کلیدی:</span></span></span></strong><span dir="RTL" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> ترمیم زخم، بابونه، کلرهگزیدین، زخم دهانی</span></span></span> <strong>Background and Objectives:</strong> Since oral ulcers impair normal oral function of the individual, finding ways to accelerate the process of wound healing is necessary. <em>Chamomilla recutita</em> (<a name="_Hlk491424752">CR</a>) is an herbal plant that has beneficial effects on healing wounds. This study was conducted to compare the effect of CR mouthwash with 0.2% chlorhexidine on linear oral ulcer healing in rats.<span dir="RTL"></span><br> <strong>Materials and Methods:</strong> For this experimental study, 24 male rats were selected and general anesthesia was done. Then the linear ulcers were made in the central region of the tongue, at a depth of 1 mm and limited to the mucosa. The animals were randomly divided into two groups: control (chlorhexidine mouthwash) and target (CR mouthwash). Each group was randomly divided into three subgroups, and the animals were sacrificed on days 3, 7 and 10. Subjects were then sampled from the lingual region and examined by the pathologist in terms of the extent of granulation tissue, angiogenesis, mean number of fibroblasts, neutrophils, lymphocytes and macrophages, and the results were recorded. Data were analyzed by Mann-Whitney test.<br> <strong>Results: </strong>Both mouthwashes acted to the same extent to accelerate the repair process. And except for the percentage of macrophages on the third day (p= 0.028), other variables did not show any significant difference.<br> <strong>Conclusion:</strong> Due to the similar effects of CR and Chlorhexidine 2% on oral wound healing and less side effects of the herbal mouthwashes, CR mouthwash<strong>Background and Objectives:</strong> Since oral ulcers impair normal oral function of the individual, finding ways to accelerate the process of wound healing is necessary. <em>Chamomilla recutita</em> (<a name="_Hlk491424752">CR</a>) is an herbal plant that has beneficial effects on healing wounds. This study was conducted to compare the effect of CR mouthwash with 0.2% chlorhexidine on linear oral ulcer healing in rats.<span dir="RTL"></span><br> <strong>Materials and Methods:</strong> For this experimental study, 24 male rats were selected and general anesthesia was done. Then the linear ulcers were made in the central region of the tongue, at a depth of 1 mm and limited to the mucosa. The animals were randomly divided into two groups: control (chlorhexidine mouthwash) and target (CR mouthwash). Each group was randomly divided into three subgroups, and the animals were sacrificed on days 3, 7 and 10. Subjects were then sampled from the lingual region and examined by the pathologist in terms of the extent of granulation tissue, angiogenesis, mean number of fibroblasts, neutrophils, lymphocytes and macrophages, and the results were recorded. Data were analyzed by Mann-Whitney test.<br> <strong>Results: </strong>Both mouthwashes acted to the same extent to accelerate the repair process. And except for the percentage of macrophages on the third day (p= 0.028), other variables did not show any significant difference.<br> <strong>Conclusion:</strong> Due to the similar effects of CR and Chlorhexidine 2% on oral wound healing and less side effects of the herbal mouthwashes, CR mouthwash can be used instead of chlorhexidine 2% to accelerate the oral linear wound healing.<br> <strong>Key words:</strong> Wound healing, <em>Chamomilla recutita</em>, Chlorhexidine, Oral wound<br> &nbsp;<br> <strong>Funding:</strong> This research was funded by Rafsanjan University of Medical Sciences.<br> <strong>Conflict of interest:</strong> None declared.<br> <strong>Ethical approval:</strong> The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.<br> &nbsp;<br> <strong>How to cite this article: </strong>Salari Sedigh S, Sadeghi M, Khademolhosseini M, Fatemi I, Rafiei A. Comparison of the Effect of <em>Chamomilla Recutita</em> and Chlorhexidine 0.2% on Oral Wound Healing in Male Rats: An Experimental Study. <em>J Rafsanjan</em> <em>Univ Med Sci</em> 2019; 18 (4): 391-400 [Farsi]<br> &nbsp;<br> can be used instead of chlorhexidine 2% to accelerate the oral linear wound healing.<br> <strong>Key words:</strong> Wound healing, <em>Chamomilla recutita</em>, Chlorhexidine, Oral wound<br> &nbsp;<br> <strong>How to cite this article: </strong>Salari Sedigh S, Sadeghi M, Khademolhosseini M, Fatemi I, Rafiei A. Comparison of the Effect of <em>Chamomilla Recutita</em> and Chlorhexidine 0.2% on Oral Wound Healing in Male Rats: An Experimental Study. <em>J Rafsanjan</em> <em>Univ Med Sci</em> 2019; 18 (4): 391-400 [Farsi]<br> &nbsp;<br> &nbsp; ترمیم زخم, بابونه, کلرهگزیدین, زخم دهانی Wound healing, Chamomilla recutita, Chlorhexidine, Oral wound 391 400 http://journal.rums.ac.ir/browse.php?a_code=A-10-2525-2&slc_lang=fa&sid=1 S. Salari Sedigh سمیه سالاری‌صدیق ssalarisedigh@gmail.com 6900319475328460034243 6900319475328460034243 Yes دانشگاه علوم پزشکی، رفسنجان، M. Sadeghi مصطفی صادقی 6900319475328460034244 6900319475328460034244 No دانشگاه علوم پزشکی، رفسنجان، M. Khademolhosseini مرتضی خادم‌الحسینی 6900319475328460034245 6900319475328460034245 No دانشگاه علوم پزشکی، رفسنجان، I. Fatemi ایمان فاطمی 6900319475328460034246 6900319475328460034246 No دانشگاه علوم پزشکی، رفسنجان، A. Rafiei عاطفه رفیعی ssalarisedigh@gmail.com 6900319475328460034247 6900319475328460034247 No دانشگاه علوم پزشکی، رفسنجان،