fa اثر تمرین تناوبی شدید بر بیان ژن‌ AKT1 و mTORc1 بطن چپ در موش‌های صحرایی دیابتی نوع 2: یک مطالعه تجربی فيزيولوژي Physiology پژوهشي Research <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چکیده</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زمینه و هدف:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از آن‌جایی که </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تمرین تناوبی شدید </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(</span></span><span dir="LTR" style="background:#FEFBF0;"><span style="font-family:times new roman,serif;">High intensity interval training</span></span><span dir="LTR" style="background:#FEFBF0;"><span style="font-family:times new roman,serif;">; </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">HIIT</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در هایپرتروفی فیزیولوژیک قلبی و در کاهش </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عوارض ناشی از کاردیو میوپاتی دیابتی مؤثر است، لذا ‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هدف این پژوهش</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تعیین تأثیر 12 هفته تمرین </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">HIIT</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر بیان ژن&shy;های</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"> AKT1 (<a href="https://en.wikipedia.org/wiki/Protein_Kinase_B" title="Protein Kinase B"><span style="text-decoration-line:none;"><span style="color:windowtext;">Protein Kinase B</span></span></a>) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Mammalian target of rapamycin) mTORc1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) در بطن چپ موش</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های صحرایی دیابتی نوع 2 بود. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مواد و روش‌ها:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دراین </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه تجربی، 21</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> سر موش نر صحرایی10 هفته‌ای با وزن20&plusmn;220 گرم، به‌صورت تصادفی در سه گروه دیابتی- تناوبی شدید، کنترل دیابتی و کنترل سالم قرار گرفتند.گروه دیابتی- تناوبی شدید، به مدت 12 هفته به تعداد 5 جلسه 30 دقیقه‌ای در هفته در قالب دویدن روی تردمیل با تکرارهای یک دقیقه‌ای و استراحت فعال 2 دقیقه&shy;ای بین هر تکرار شرکت داده شدند و 48 ساعت پس از آخرین جلسه تمرینی تشریح شدند</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">.</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> جهت تفسیر بیان نسبی ژن</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">&nbsp;Akt1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">mTORc1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، از آزمون </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">t</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مستقل در سطح معنی‌داری 05/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">P&le;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> استفاده شد.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته‌ها: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقایسه گروه‌‌ها</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> کاهش معنی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دار </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیان ژن&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Akt1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (007/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">(P=</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">mTORc1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> (022/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">(P=</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> درگروه کنترل دیابتی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، نسبت به گروه سالم را نشان داد. هم‌چنین تمرین منجر به افزایش معنی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دار بیان </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Akt1</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(003/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">(P =</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به میزان 72 درصد و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">&nbsp;mTORc1</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نسبت به گروه کنترل دیابتی گردید </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(001/0</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">(P=</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتیجه‌گیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به نظر می‌رسد که یک دوره </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">HIIT</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، با افزایش بیان ژن&shy;های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Akt1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">mTORc1</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> منجر به بهبود عملکرد قلبی می</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شود و احتمالاً می‌تواند از علائم بالینی ناشی از دیابت پیش‌گیری کند.</span></span><br> <span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"><strong>واژه‌ها</strong></span></span></span><strong><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ی کلیدی</span></span></span></strong><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">:</span></span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دیابت، تمرین تناوبی شدید، بیماری قلبی، </span></span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موش صحرایی،</span></span></span> <span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Akt1</span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mTORc1</span></span> <p style="margin: 0cm 0cm 0pt; text-align: justify;"><strong>Background and Objectives:</strong> High intensity interval trainings (HIIT) are effective in the physiological cardiac hypertrophy and in reducing the complications of diabetic cardiomyopathy. Therefore, the purpose of this study was to determine the effects of 12 weeks high intensity interval training on the expression of AKT1(<a href="https://en.wikipedia.org/wiki/Protein_Kinase_B" title="Protein Kinase B">Protein Kinase B</a>) and mTORc1<span dir="RTL">)</span>mammalian target of rapamycin<span dir="RTL">(</span> genes in the left ventricle of type 2 diabetic rats.<br> <strong>Materials and Methods:</strong> In this experimental study, 21 male Wistar rats weighting 220&plusmn;20 gr were randomly divided into three groups including diabetic-HIIT, diabetic control, and healthy control group. The diabetic HIIT group have been participated in 5 sessions (30 minutes per week) in the form of running on a treadmill with repetitions of 1 minute and 2 minutes active rest between each repetition for 12 weeks. All rats were sacrificed 48 hours after the last training session. To compare relative expression of AKT1 and mTORc1 genes, independent t-test was used at a significant level of p&le;0.05.<br> <strong>Results: </strong>Comparing the groups showed a significant decrease in the expression of Akt1 (p=0.007<span dir="RTL">(</span> and&nbsp; mTORc1(p=0.022) genes in the diabetic control group compared to the healthy group. The training also significantly increased the expression of Akt1 (p=0.003) (up to 72%) and mTORc1 genes (p=0.001) compared to the diabetic control group.<span dir="RTL"></span><br> <strong>Conclusion</strong><strong>:</strong> It seems that HIIT increases the gene expression of Akt1 and mTORc1 which can improve cardiac function and may probably prevent clinical &nbsp;symptom of heart disease resulted from diabetes.<br> <strong>K</strong><strong>ey words</strong><strong>:</strong> Diabetes, High intensity interval training, Heart disease, Rat, Akt1, mTORc1.<span dir="RTL"></span><br> &nbsp;<br> <strong>Funding</strong>: This study did not have any funds.<br> <strong>Conflict of interest</strong>: None declared.<br> <strong>Ethical approval:</strong> The Ethics Committee of Islamic Azad University, Tehran South Branch approved the study (IR.IAU.TEHRAN.REC.51/410/16).<br> &nbsp;<br> <strong>How to cite this article: </strong>Mirsepasi M, Baneifar AB, Azarbayjani MA, Arshadi S. The Effects of High Intensity Interval Training on Gene Expression of AKT1 and mTORc1 in the Left Ventricle of Type 2 Diabetic Rats: An Experimental Study. <em>J Rafsanjan</em> <em>Univ Med Sci</em> 2019; 17 (12): 1119-30. [Farsi]<br> &nbsp;</p> دیابت, تمرین تناوبی شدید, بیماری قلبی, موش صحرایی, Akt1, mTORc1 Diabetes, High intensity interval training, Heart disease, Rat, Akt1, mTORc1 1119 1130 http://journal.rums.ac.ir/browse.php?a_code=A-10-3737-1&slc_lang=fa&sid=1 M. Mirsepasi منا میرسپاسی mona.mirsepasi@gmail.com 6900319475328460032506 6900319475328460032506 No Islamic Azad University دانشگاه آزاد اسلامی A. A. Baneifar عبدالعلی بنایی فر Abdolalibaneifar@yahoo.com 6900319475328460032507 6900319475328460032507 Yes Islamic Azad University دانشگاه آزاد اسلامی M. A. Azarbayjani محمد علی آذربایجانی Ali.Aliazarbayjani@gmail.com 6900319475328460032508 6900319475328460032508 No Islamic Azad University دانشگاه آزاد اسلامی S. Arshadi سجاد ارشدی Arshadi.Sajad@yahoo.com 6900319475328460032509 6900319475328460032509 No Islamic Azad University دانشگاه آزاد اسلامی