fa مطالعه اثرضد مالاریایی عصاره هیدروالکلی گیاه زنجبیل (Zingiber Officinale) در موش‌های آلوده به پلاسمودیوم برگئی: یک مطالعه تجربی انگل شناسي Parasitology پژوهشي Research <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چکیده</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زمینه و هدف</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">:</span></span></strong> <span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">با توجه به مقاومت انگل پلاسمودیوم عامل بیماری مالاریا نسبت به داروهای ضد مالاریایی، یافتن دارویی جایگزین برای درمان بیماران امری ضروری به&shy;نظر می&shy;آید. لذا مطالعه حاضر با هدف </span></span></span><a name="_Hlk528627064"><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">مطالعه اثر</span></span></span></a> <span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">ضد مالاریایی عصاره هیدروالکلی گیاه زنجبیل (</span></span></span><em><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">Zingiber Officinale</span></span></em><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">) در موش&shy;های آلوده به پلاسمودیوم برگئی</span></span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> انجام شد.</span></span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"></span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">مواد و روش</span></span></span></strong><strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">‌</span></span></span></strong><strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">ها:</span></span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> در مطالعه تجربی حاضر، 40 سر موش به پلاسمودیوم برگئی آلوده شدند و طی چهار روز متوالی با غلظت&shy;های 50، 100، 150، 200 و250 میلی&shy;گرم بر میلی&shy;لیتر عصاره هیدروالکلی گیاه زنجبیل مورد درمان قرار گرفتند. تست سمیت بر روی کبد و کلیه موش&shy;ها انجام شد. در آخر داده&shy;ها از لحاظ آماری با آزمون&shy;های </span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">t</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> زوجی و آنالیز واریانس یک&shy;طرفه تجزیه و تحلیل شدند.</span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">یافته</span></span></span></strong><strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">‌</span></span></span></strong><strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">ها:</span></span></span></strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> عصاره هیدروالکلی گیاه زنجبیل در دوز درمانی 250 میلی&shy;گرم اثر مهاری 62 درصدی بر رشد انگل در موش سوری داشت که از نظر آماری اختلاف آن با گروه کنترل معنی&shy;دار بود (05/0></span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">P</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">). بیش&shy;ترین میزان بقاء در موش&shy;ها مربوط به غلظت 250 میلی&shy;گرم با میانگین 2</span></span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:cambria,serif;"><span style="font-size:12pt;">&plusmn;</span></span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> 24 روز بود که اختلاف آن نسبت به سایر گروه&shy;های درمانی معنی&shy;دار بود (05/0></span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">P</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">). مقایسه کبد و کلیه موش&shy;ها با گروه کنترل منفی نشان داد عصاره فوق فاقد سمیت برای این ارگان&shy;ها می&shy;باشد (071/0</span></span></span><span dir="LTR" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:times new roman,serif;">P=</span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">).</span></span></span><br> <strong><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">نتیجه&shy;گیری:</span></span></span></strong> <a name="_Hlk529058867"><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">با توجه به تأثیر 62 درصدی گیاه زنجبیل در درمان</span></span></span></a><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;"> موش&shy;های آلوده به پلاسمودیوم برگئی</span></span></span><span style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">، تحقیقات بیشتر در زمینه استفاده از این گیاه در درمان انگل مالاریا با افزایش دوز درمانی و یا تهیه عصاره&shy;های مختلف کلروفرمی و آبی آن پیشنهاد می&shy;شود.</span></span></span><br> <strong><span dir="RTL" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">واژه&shy;های کلیدی:</span></span></span></strong> <span dir="RTL" style="background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-attachment:initial;background-origin:initial;background-clip:initial;"><span style="font-family:b nazanin;"><span style="font-size:12pt;">پلاسمودیوم برگئی، گیاه زنجبیل، عصاره هیدروالکلی، موش سوری</span></span></span> <strong>Background and Objectives:</strong> Considering the resistance of the Plasmodium parasite (the causative agent of Malaria) to antimalarial drugs, it is essential to find an alternative medicine for treating patients. Therefore, the present study aimed to investigate the antimalarial effect of Zingiber Officinale hydroalcoholic extract on mice infected with Plasmodium berghei.<br> <strong>Materials and Methods:</strong> In this experimental study, a number of 40 mice were infected with Plasmodium berghei and treated with different concentrations of the hydro-alcoholic extract of ginger plant (50, 100, 150, 200, 250 mg/ml) for four consecutive days. Toxicity testing was performed on liver and kidneys (hepatic and renal tissues) of mice. Finally, Data were statistically analyzed using the paired t-test and one-way analysis of variance (ANOVA).<br> <strong>Results: </strong>The results of this study showed that the hydro-alcoholic extract of ginger at a therapeutic dose of 250 mg/kg, had a suppressive effect (62%) on the growth of parasite in the mice, which was significantly different from the control group (p<0.05). The highest survival rate in the mice was at a concentration of 250 mg/kg with an average of 24&plusmn; 2 days, which was significantly different from the other treatment groups (p<0.05). Comparing liver and kidney of mice with the negative control group showed that the extract had no toxicity for these organs (p=0.071).<br> <strong>Conclusion</strong>: Considering the treatment effect (62%) of Ginger plant on the mice infected with Plasmodium berghei, further researches on the use of this plant in the treatment of malaria parasite with increasing the therapeutic dosage of the drug or preparation of various chloroformic and water extracts of the plant is recommended.<br> <strong>Key words</strong>: Plasmodium berghei, Ginger plant, Hydroalcoholic extract, Mice.<br> &nbsp;<br> <strong>Funding: </strong>This research was funded by the Iran University of Medical Sciences, Tehran, Iran.<br> <strong>Conflict of interest</strong>: None declared<br> <strong>Ethical approval:</strong> The Ethics Committee of Iran University of Medical Sciences approved the study (IR.IUMS.FMD.REC.1397.219).<br> &nbsp;<br> <strong>How to cite this article:</strong> Elmi T, Hajialiani F, Asadi M R, Orujzadeh F, Kalantari Hesari A, Rahimi Esboei B, Gholami Sh. A Study on the Effect of Zingiber Officinale Hydroalcoholic Extract on <em>Plasmodium berghei</em> in Infected Mice: An Experimental Study. <em>J Rafsanjan Univ Med Sci</em> 2019; 18 (4): 353-64. [Farsi]<br> &nbsp;<br> &nbsp; پلاسمودیوم برگئی, گیاه زنجبیل, عصاره هیدروالکلی, موش سوری Plasmodium berghei, Ginger plant, Hydroalcoholic extract, Mice 353 364 http://journal.rums.ac.ir/browse.php?a_code=A-10-1880-3&slc_lang=fa&sid=1 T. Elmi طاهر علمی elmi1364@yahoo.com 6900319475328460034114 6900319475328460034114 No Iran University of Medical Sciences دانشکاه علوم پزشکی ایران F. Hajialiani فاطمه حاجی علیانی fateme.aliani82@gmail.com 6900319475328460034115 6900319475328460034115 No Iran University of Medical Sciences دانشکاه علوم پزشکی ایران M. R. Asadi محمدرضا اسدی fariba_1388@yahoo.com 6900319475328460034116 6900319475328460034116 No Iran University of Medical Sciences دانشکاه علوم پزشکی ایران F. Orujzadeh فریبا اورج زاده elmit1364@gmail.com 6900319475328460034117 6900319475328460034117 No Tehran University of Medical Sciences دانشگاه تهران A. Kalantari Hesari علی کلانتری حصاری kalantari.ali7@gmail.com 6900319475328460034118 6900319475328460034118 No Tehran University of Medical Sciences دانشگاه تهران B. Rahimi Esboei بهمن رحیمی اسبوئی sgholami200@gmail.com 6900319475328460034119 6900319475328460034119 No Mazandaran University of Medical Sciences دانشگاه علوم پزشکی مازندران Sh. Gholami شیرزاد غلامی 6900319475328460034120 6900319475328460034120 Yes دانشگاه علوم پزشکی مازندران